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Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

A New Liquid Homogeneous Assay for HDL Cholesterol Determination Evaluated in Seven Laboratories in Europe and the United States

  • M. Nauck , M. S. Graziani , J. Jarausch , D. Bruton , C. Cobbaert , T. G. Cole , F. Colella , F. Lefevre , P. Gillery , B. Haas , T. Law , M. König , M. Macke , W. März , C. Meier , W. Riesen , M. van Vliet , H. Wieland and N. Rifai
From the journal


We evaluated a new liquid homogeneous assay for the direct measurement of high density lipoprotein cholesterol (HDL-C Plus) in seven laboratories. The assay includes two reagents which can be readily used in most available clinical chemistry analyzers.

The total CVs of the new method were below 4.6% and the bias in relation to the designated comparison method was below 3.9%. The total error ranged between 4 to 7%. HDL-C values determined by this method were in good agreement with those obtained by the old homogeneous assay using lyophilized reagents, and other homogeneous and precipitation assays (0.944 < r < 0.996). The assay was linear up to at least 3.89 mmol/l HDL-C.

Hemoglobin did not interfere, whereas in icteric samples slight deviations were observed. Lipemia up to 11.3 to 22.6 mmol/l triglycerides did not interfere with this homogeneous HDL-C assay. In samples of patients with paraproteinemia, discrepant results were seen. This liquid homogeneous HDL-C assay was easy to handle and produced similar results in all laboratories participating in this study. This method will enable clinical laboratories to reliably measure HDL-C for risk assessment of coronary heart disease.

Published Online: 2005-06-01
Published in Print: 1999-11-18

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

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