Accessible Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Biochemical Factors Influencing Measurement of Cardiac Troponin I in Serum

A. Katrukha, A. Bereznikova, V. Filatov and T. Esakova
From the journal

Abstract

Troponin I (cTnI), a sensitive and reliable marker of damaged cardiac tissue, is now widely used in clinics. But the existence of different cTnI assays with a wide variety of cut-off values and discrepancies between the results of measurements of one and the same sample by different assays is puzzling for clinicians. The most urgent issue at the moment is the development of the international standard, which can be used for the calibration of different assays, thus decreasing between assay biases. But another important item, which should be considered by manufacturers, is the standardisation of the epitopes of the antibodies used for the assay development. The importance of such standardisation originates from the complicated biochemical nature of cTnI. Here we briefly try to analyse the main factors that can influence antigen recognition by different antibodies and formulate principles of antibody selection for assay development.

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Published Online: 2005-06-01
Published in Print: 1999-11-18

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG