Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Determination of H+/K+-ATPase Activity in Human Gastric Biopsy Specimens

István Kocsis, Barna Vásárhelyi, Zsolt Tulassay, Teréz Szabó, Ágota Vér and Tivadar Tulassay
From the journal

Abstract

The aim of our work was to develop a method to determine the the H+/K+-ATPase activity of human gastric biopsy samples.

Our method is based on the phosphatase activity and the K+-induceable property of the enzyme. K+-induceable pNPPase activity was determined from homogenated corpus and antrum biopsy samples. H+/K+-ase activity was calculated as the difference between the corpus and antrum K+-induceable pNPPase activities.

Quality control measurements were done during 20 successive days from pooled homogenates. The total, between-day and between-run, within-day and within-run coefficients of variations were between 10 and 16%. The healthy mean and reference range of K+-induceable pNPPase activity in the corpus was 95.8 (95% CI: 83.4–108.2 mU/mg protein); in the antrum it was 28.3 (21.6–35.0) mU/mg protein. The calculated H+/K+-ATPase activity was 67.2 (56.9–77.5) mU/mg protein. The measured activities were independent of the age and gender.

Summarizing our results we have concluded, that our novel method might be a potential tool to gather data about the functional acid producing capability of human gastric mucosa.

:
Published Online: 2005-06-01
Published in Print: 2000-08-21

Copyright © 2000 by Walter de Gruyter GmbH & Co. KG

Scroll Up Arrow