Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Precision and Accuracy of a HPLC Method for Measurement of Fecal Porphyrin Concentrations

  • Floris M. J. Zuijderhoudt , Johannes S. Kamphuis , Willem E. Kluitenberg and Jenneke Dorresteijn-de Bok


We studied precision and accuracy of a HPLC method for determination of porphyrins in feces. A commercial standard solution appeared to contain less coproporphyrin (15%) than stated by the manufacturer. The between-batch coefficients of variation were often below 15% and were higher than the within-batch coefficients. The precision of porphyrin measurements was not influenced by the type of porphyria. Recoveries of added coproporphyrin and protoporphyrin were 90% and 108%; coefficients of variation were 6% and 19%, respectively.

Published Online: 2005-06-01
Published in Print: 2002-10-29

Copyright © 2002 by Walter de Gruyter GmbH & Co. KG

Downloaded on 2.12.2023 from
Scroll to top button