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Accessible Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Rapid and Reliable Genotyping of the C181T Polymorphism in the Bradykinin B2 Receptor Gene

Johanna Weiss, Christiane Hesse and Walter Emil Haefeli
From the journal


Bradykinin is not only a mediator of pain and inflammation but also a potent vasodilator and is likely to contribute to the cardioprotective effects of angiotensin-converting enzyme inhibitors. The B2 receptor (B2-R) mediating most of the inflammatory and cardiovascular effects of bradykinin represents a candidate gene likely involved in the complex genetic causes of common chronic disorders such as hypertension. The C181T polymorphism of the B2-R gene influences the potency of bradykinin and there is evidence of a possible role of this polymorphism in the development of hypertension and end-stage renal disease. We developed and validated a new, rapid, and reliable method for the detection of the C181T polymorphism based on the hybridization probes format on the LightCycler™, which is superior to the conventional PCR-RFLP method commonly used to detect this polymorphism. It allows the solid evaluation of large patient populations with the best time.

Published Online: 2005-06-01
Published in Print: 2003-03-24

Copyright (c) 2003 by Walter de Gruyter GmbH & Co. KG