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Licensed Unlicensed Requires Authentication Published by De Gruyter September 21, 2011

Effect of prolonged physical exercise on urinary proANP1-30 and proANP31-67

  • Enrico Cappellin , Elio F. De Palo , Rosalba Gatti , Giuseppe Soldà , Wolfgang Woloszczuk and Paolo Spinella


Dynamic exercise strongly affects atrial natriuretic peptides (ANP), in particular the mature bioactive αANP and the proANP fragments, namely proANP1–98, proANP1–30 and proANP31–67. The proANPs influence kidney functions and their plasma levels increase after physical exercise. We measured urinary proANP1–30 and proANP31–67 levels before and at the end of physical exercise in 28 well-trained male cyclists. For the first time, the proANP1–30 and proANP31–67 urinary levels in athletes before and at the end of a prolonged agonistic bicycle race were measured. Urinary creatinine and total proteins were also measured. The urinary proANP31–67, creatinine and total protein levels were significantly higher at the end of exercise than before. In contrast, proANP1–30/protein and proANP31–67/protein ratios decreased after exercise. Even proANP1–30/creatinine and proANP31–67/creatinine ratios were lower after exercise. A significant correlation between proANP1–30 and proANP31–67 urinary levels at the end of exercise was found. The proANP31–67/creatinine ratio before and after exercise also showed a significant correlation. The variation of urinary proANP fragments confirmed their possible role in physical exercise. In particular, it could be interpreted as a response of the body or kidney to renal impairment occurring during exercise.

Corresponding author: Prof. Elio Franco De Palo, CD, Section of Clinical Biochemistry, Department of Medical Diagnostic Sciences and Spec. Ther., University of Padua, c/o ex Istituto di Semeiotica Medica, Via Ospedale 105, 35128 Padova, Italy. Phone: (+39)-049-8213016, Fax: (+39)-049-8272485, E-mail:


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Received: 2004-4-13
Accepted: 2004-6-15
Published Online: 2011-9-21
Published in Print: 2004-9-1

© Walter de Gruyter

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