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Licensed Unlicensed Requires Authentication Published by De Gruyter July 5, 2005

Spectrophotometric determination of urinary iodine by the Sandell-Kolthoff reaction subsequent to dry alkaline ashing. Results from the Czech Republic in the period 1994–2002

Radovan Bílek, Jiří Bednář and Václav Zamrazil

Abstract

The Czech Republic is an iodine-deficient area. Insufficient iodine intake was reduced by enriching cooking salt with iodine in the range 20–34mgI/kg. An important indicator for tracking changes in iodine nutrition over time is accurate information about urinary iodine concentrations in the population. In this paper we describe and characterize our method used for the determination of iodine in biological material, which is based on alkaline ashing of urine specimens preceding Sandell-Kolthoff reaction using brucine as a colorimetric marker. The losses of radioiodine added during sample preparation have not exceeded 0.001%. The detection limit is 2.6μgI/L and the limit of quantification is 11.7μgI/L, with intra-assay precision of 4% and inter-assay precision of 4.9%. During the period 1994–2002, the urinary iodine concentration was determined in 29,612 samples in the Institute of Endocrinology. The mean basal urinary iodine concentrations±SD were 115±69μgI/L. Of the samples, 0.7% were in severe (<20μgI/L), 9.6% in moderate (20–49μgI/L), 40.1% in mild (50–99μgI/L), 35.6% in adequate (100–200μgI/L), and 14.0% in more than adequate (>200μgI/L) subsets of iodine nutrition. A statistically significant (p<0.00001) difference was found between mean male (127μgI/L) and female (112μgI/L) urinary iodine, and an inversely proportional trend also exists in the age-related data.


Corresponding author: Radovan Bílek, Ing, PhD, Institute of Endocrinology, Národní 8, 116 94 Prague 1, Czech Republic Phone: +42-2-24905251, Fax: +42-2-24905325,

References

1 Delange F. The disorders induced by iodine deficiency. Thyroid 1994; 4: 107–28. 10.1089/thy.1994.4.107Search in Google Scholar PubMed

2 Hetzel BS. The iodine deficiency disorders. In: Delange F, Dunn JT, Glioner D, editors. Iodine deficiency in Europe. NATO ASI Series A: Life Sciences, vol. 241. New York: Plenum Press, 1993:25. Search in Google Scholar

3 Maberly GF. Iodine deficiency disorders: contemporary scientific issues. J Nutr 1994; 124(Suppl 8): 1473–8. 10.1093/jn/124.suppl_8.1473SSearch in Google Scholar PubMed

4 Delange F. Iodine deficiency in Europe and its consequences: an update. Eur J Nucl Med Mol Imaging 2002; 29(Suppl 2): 404–16. 10.1007/s00259-002-0812-7Search in Google Scholar PubMed

5 Vitti P, Rago T, Aghini-Lombardi F, Pinchera A. Iodine deficiency disorders in Europe. Public Health Nutr 2001; 4: 529–35. 10.1079/PHN2001138Search in Google Scholar PubMed

6 Hetzel BS. Eliminating iodine deficiency disorders – the role of the International Council in the global partnership. Bull World Health Organ 2002; 80: 410–3. Search in Google Scholar

7 Silink K, Reinsenauer R, Chaloupsky J. The problem of mapping endemic goiter in Czechoslovakia. Rev Czech Med 1959; 5: 73–88. Search in Google Scholar

8 Conference “Standardization of ultrasound and urinary iodine determination for assessing iodine status”: report of a technical consultation. IDD Newsl 2000; 16: 19–23. Search in Google Scholar

9 Bednář J, Rohling S, Vohnout S. Contribution to determining protein iodine in the blood serum. Cesk Farm 1964; 13: 203–9. Search in Google Scholar

10 May W, Wu D, Eastman C, Bourdoux P, Maberly G. Evaluation of automated urinary iodine methods: problems of interfering substances identified. Clin Chem 1990; 36: 865–9. 10.1093/clinchem/36.6.865Search in Google Scholar

11 Ford HC, Johnson LA. Ascorbic acid interferes with an automated urinary iodide determination based on the ceric-arsenious acid reaction. Clin Chem 1991; 37: 759. 10.1093/clinchem/37.5.759Search in Google Scholar

12 Pino S, Fang SL, Braverman LE. Ammonium persulfate: a new and safe method for measuring urinary iodine by ammonium persulfate oxidation. Exp Clin Endocrinol Diabetes 1998; 106(Suppl 3): 22–7. 10.1055/s-0029-1212041Search in Google Scholar PubMed

13 Dunn JT, Crutchfield HE, Gutekunst R, Dunn AD. Methods for measuring iodine in urine. International Council for Control of Iodine Deficiency Disorders. ICCIDD/UNICEF/WHO: The Netherlands, 1993:58–62. Search in Google Scholar

14 Pino S, Fang SL, Braverman LE. Ammonium persulfate: a safe alternative oxidizing reagent for measuring urinary iodine. Clin Chem 1996; 42: 239–43. 10.1093/clinchem/42.2.239Search in Google Scholar

15 Fallouch S, Lejeune PJ, Barbaria J, Carayon P, Mallet B. Urinary iodine analysis: an alternative method for digestion of urine samples. Clin Chem 2004; 50: 780–2. 10.1373/clinchem.2003.029066Search in Google Scholar PubMed

16 Dunn JT, Crutchfield HE, Gutekunst R, Dunn AD. Two simple methods for measuring iodine in urine. Thyroid 1993; 3: 119–23. 10.1089/thy.1993.3.119Search in Google Scholar PubMed

17 Tomiyasu T, Nonaka M, Uchikado M, Anazawa K, Sakamoto H. Kinetic determination of total iodine in urine and foodstuffs using a mixed acid as a pretreatment agent. Anal Sci 2004; 20: 391–3. 10.2116/analsci.20.391Search in Google Scholar

18 Tsuda K, Namba H, Nomura T, Yokoyama N, Yamashita S, Izumi M, et al. Automated measurement of urinary iodine with use of ultraviolet irradiation. Clin Chem 1995; 41: 581–5. 10.1093/clinchem/41.4.581Search in Google Scholar

19 Sandell EB, Kolthoff IM. Micro determination of iodine by catalytic method. Mikrochim Acta 1937; 1: 9–25. 10.1007/BF01476194Search in Google Scholar

20 Allain P, Mauras Y, Douge C, Jaunault L, Delaporte T, Beaugrand C. Determination of iodine and bromine in plasma and urine by inductively coupled plasma mass spectrometry. Analyst 1990; 115: 813–5. 10.1039/AN9901500813Search in Google Scholar

21 Haldimann M, Zimmerli B, Als C, Gerber H. Direct determination of urinary iodine by inductively coupled plasma mass spectrometry using isotope dilution with iodine-129. Clin Chem 1998; 44: 817–24. 10.1093/clinchem/44.4.817Search in Google Scholar

22 Dermelj M, Slejkovec Z, Byrne AR, Stegnar P, Hojker S, Porenta M, et al. Rapid radiochemical neutron activation analysis for iodine in urine by different separation techniques. Analyst 1992; 1: 443–6. 10.1039/AN9921700443Search in Google Scholar

23 Andersson S, Forsman U. Determination of total iodine in biological material by alkaline ashing and column-switching ion-pair liquid chromatography. J Chromatogr B 1997; 692: 53–9. 10.1016/S0378-4347(96)00483-5Search in Google Scholar

24 Odink J, Bogaards JJ, Sandman H, Egger RJ, Arkesteyn GA, de Jong P. Excretion of iodide in 24-h urine as determined by ion-pair reversed-phase liquid chromatography with electrochemical detection. J Chromatogr 1988; 431: 309–16. 10.1016/S0378-4347(00)83099-6Search in Google Scholar

25 Li HB, Chen F, Xu XR. Determination of iodide in seawater and urine by size exclusion chromatography with iodine-starch complex. J Chromatogr A 2001; 918: 335–9. 10.1016/S0021-9673(01)00776-2Search in Google Scholar

26 Yabu Y, Miyai K, Endo Y, Hata N, Iijima Y, Hayashizaki S, et al. Urinary iodide excretion measured with an iodide-selective ion electrode: studies on normal subjects of varying ages and patients with thyroid diseases. Endocrinol Jpn 1988; 35: 391–8. 10.1507/endocrj1954.35.391Search in Google Scholar PubMed

27 Pantuckova P, Krivankova L. Fast and simple method for determination of iodide in human urine, serum, sea water, and cooking salt by capillary zone electrophoresis. Electrophoresis 2004; 25: 1102–10. 10.1002/elps.200305805Search in Google Scholar PubMed

28 Yaping Z, Dongxing Y, Jixiang C, Tianshiu L, Huiqin C. Spectrophotometric determination of urinary iodine by flow-injection analysis with on-line catalytic digestion. Clin Chem 1996; 42: 2021–7. 10.1093/clinchem/42.12.2021Search in Google Scholar

29 Bilabina I, Brazier M, Bour H, Doh A, Desmet G. Evaluation of iodide deficiency in Togo using an optimized potentiometric method for iodide estimation in urine. Ann Biol Clin (Paris) 1994; 52: 261–4. Search in Google Scholar

30 Ohashi T, Yamaki M, Pandav CS, Karmarkar MG, Irie M. Simple microplate method for determination of urinary iodine. Clin Chem 2000; 46: 529–36. 10.1093/clinchem/46.4.529Search in Google Scholar

31 Wuethrich C, Jaeggi-Groisman SE, Gerber H. Comparison of two methods for the detection of urinary iodine used in epidemiological studies. Clin Chem Lab Med 2000; 38: 1027–31. 10.1515/CCLM.2000.152Search in Google Scholar PubMed

32 Rendl J, Bier D, Groh T, Reiners C. Rapid urinary iodide test. J Clin Endocrinol Metab 1998; 83: 1007–12. 10.1210/jcem.83.3.4633Search in Google Scholar PubMed

33 Dunn JT, Myers HE, Dunn AD. Simple methods for assessing urinary iodine, including preliminary description of a new rapid technique (“Fast B”). Exp Clin Endocrinol Diabetes 1997; 106(Suppl 3): 10–2. 10.1055/s-0029-1212037Search in Google Scholar PubMed

34 Gnat D, Dunn AD, Chaker S, Delange F, Vertongen F, Dunn JT. Fast colorimetric method for measuring urinary iodine. Clin Chem 2003; 49: 186–8. 10.1373/49.1.186Search in Google Scholar PubMed

35 Plantin-Carrenard E, Cattan F, Aurengo A, Dumerat B, Foglietti MJ, Beaudeux JL. Analytical aspects of the semiquantitative determination of urinary iodine using ferroin: interest for a rapid screening of iodine deficiency or excess. Ann Biol Clin (Paris) 2004; 62: 456–61. Search in Google Scholar

36 Hintze J. NCSS and PASS, Number Cruncher Statistical System. http://www.ncss.com (accessed 2001). Search in Google Scholar

37 Delange F, Podoba J, Vertongen F, Ott W. Thyromobil project: standardized iodine deficiency evaluation in Europe. IDD Newsl 1995; 11: 33–4. Search in Google Scholar

38 Delange F, de Benoist B, Burgi H. Determining median urinary iodine concentration that indicates adequate iodine intake at population level. Bull World Health Organ 2002; 80: 633–6. Search in Google Scholar

39 Dahl L, Opsahl JA, Meltzer HM, Julshamn K. Iodine concentration in Norwegian milk and dairy products. Br J Nutr 2003; 90: 679–85. 10.1079/BJN2003921Search in Google Scholar

40 Als C, Haldimann M, Burgi E, Donati F, Gerber H, Zimmerli B. Swiss pilot study of individual seasonal fluctuations of urinary iodine concentration over two years: is age-dependency linked to the major source of dietary iodine? Eur J Clin Nutr 2003; 57: 636–46. 10.1038/sj.ejcn.1601590Search in Google Scholar PubMed

41 WHO. Assessment of iodine deficiency disorders and monitoring their elimination, 2nd ed. Publication WHO/NHD/01.1, 2001. Search in Google Scholar

42 Zamrazil V, Bílek R, Čerovska J, Delange F. The elimination of iodine deficiency in the Czech Republic: the steps towards success. Thyroid 2004; 14: 49–56. 10.1089/105072504322783849Search in Google Scholar PubMed

Received: 2004-10-13
Accepted: 2005-4-1
Published Online: 2005-7-5
Published in Print: 2005-6-1

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