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Licensed Unlicensed Requires Authentication Published by De Gruyter September 21, 2011

Sensitive immunoassays for the autoantibodies reacting against citrullinated carboxy-terminal telopeptides of type I and type II collagens in patients with rheumatoid arthritis

Marja-Kaisa Koivula , Jarmo Ramberg , Sari Åman , Anna Karjalainen , Markku Hakala and Juha Risteli


We developed sensitive assay methods for autoantibodies recognizing the citrullinated synthetic peptides derived from type I and type II collagens in patients with rheumatoid arthritis (RA). These peptides were tested with the chemiluminescence method (Nichols Advantage® System). In 44 RA patients out of 120, the sera showed increased binding of citrullinated synthetic C-telopeptide derived from the α1 chain of type I collagen (p=0.003 compared to controls). For a corresponding C-telopeptide pair from the α1 chain of type II collagen, 35 patient sera bound the citrullinated peptide more strongly than the arginine peptide, but the difference compared to the controls was not significant (p=0.074). Correlation between the two carboxy-telopeptides was r=0.473 (p<0.001). The anti-CCP assay (antibodies against citrullinated filaggrin sequence-derived peptides) was positive in 59% of our RA patients. There was no relationship between the anti-CCP results and the antibodies against collagen C-telopeptides, but both are increased in RA patients. We demonstrated autoantibodies in RA patients that bound citrullinated C-telopeptides derived from type I and type II collagen antigens. The peptide sequences detected (-YYXA and -YMXA) were different from that based on the cyclic filaggrin antigen (-STXG-, where X represents citrulline).

Corresponding author: Juha Risteli, Department of Clinical Chemistry, University of Oulu, PL 5000, 90014 Oulu yliopisto, Finland Fax +358-5-3154451,


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Received: 2005-6-16
Accepted: 2005-9-1
Published Online: 2011-9-21
Published in Print: 2005-12-1

©2005 by Walter de Gruyter Berlin New York

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