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Licensed Unlicensed Requires Authentication Published by De Gruyter September 21, 2011

Comparison of the TaqMan and LightCycler systems in pharmacogenetic testing: evaluation of CYP2C9*2/*3 polymorphisms

Mario Toriello, Pasquale Meccariello, Cristina Mazzaccara, Rosanna Di Fiore, Carmela Esposito and Lucia Sacchetti


Background: Pharmacogenetic testing for drug-metabolizing enzymes is not yet widely used in clinical practice.

Methods: In an attempt to facilitate the application of this procedure, we have compared two real-time PCR-based methods, the TaqMan® and the LightCycler™ for the pharmacogenetic evaluation of CYP2C9*2/*3 polymorphisms.

Results and Conclusion: Both procedures are suitable for pharmacogenetic studies. The TaqMan procedure was less expensive in terms of cost per sample, but the TaqMan apparatus is more expensive than the LightCycler apparatus.

Corresponding author: Lucia Sacchetti, Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, via S. Pansini 5, 80131 Naples, Italy Phone: +39-081-7463532, Fax: +39-081-7462404,


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Received: 2005-7-27
Accepted: 2005-12-1
Published Online: 2011-9-21
Published in Print: 2006-3-1

©2006 by Walter de Gruyter Berlin New York