Accessible Unlicensed Requires Authentication Published by De Gruyter September 21, 2011

Comparison of TEST 1 with SRS 100 and ICSH reference method for the measurement of the length of sedimentation reaction in blood

Sebahat Ozdem, Halide S. Akbas, Levent Donmez and Meral Gultekin

Abstract

Background: We evaluated the measurement of length of sedimentation reaction in blood (LSRB) by TEST 1 and compared the results with those for the Westergren and Sed Rate Screener 100 (SRS 100) methods.

Methods: LSRB was measured in 113 paired blood samples.

Results: TEST 1 correlated significantly with the Westergren (r=0.94) and SRS 100 (r=0.90) methods with low bias (−0.29 and −1.92mm/h, respectively) and limits of agreement (−14.5 to 13.9, and −23.4 to 19.6mm/h, respectively). Hematocrit (Htc) correlated negatively with LSRB in TEST 1 (r=−0.54) and SRS 100 (r=−0.53) only in samples with high Htc (≥35%). The bias and limits of agreement between TEST 1 and Westergren in samples with low (−1.46 and −22.3 to 19.3mm/h) and high (0.43 and −7.29 to 8.14mm/h) Htc were comparable to those between SRS 100 and Westergren (1.83 and −27.2 to 30.9mm/h for low, 0.71 and −7.27 to 8.70mm/h for high Htc samples). Total protein and fibrinogen correlated similarly with LSRB in both TEST 1 (r=0.23 and 0.48, respectively) and SRS 100 (r=0.30 and 0.51, respectively).

Conclusions: The findings suggested that TEST 1 is a reliable, precise and accurate system for measurement of LSRB in clinical laboratories with high workload.


Corresponding author: Sebahat Ozdem, Akdeniz University, Medical Faculty, Central Laboratory, Clinical Biochemistry Unit, Arapsuyu 07070, Antalya, Turkey Phone: +90-242-2274343 ext 33252, Fax: +90-242-2272535,

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Received: 2005-11-30
Accepted: 2006-1-4
Published Online: 2011-9-21
Published in Print: 2006-4-1

©2006 by Walter de Gruyter Berlin New York