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Licensed Unlicensed Requires Authentication Published by De Gruyter April 1, 2008

Cell lines that express membrane-associated DNA for anti-DNA antibody detection

Elio Tonutti, Nadia Blasone, Daniela Visentini, Monica Poletto, Danilo Villalta, Renato Tozzoli and Nicola Bizzaro

Abstract

Background: In this study, we evaluated the analytical reliability and clinical sensitivity of two tests (indirect immunofluorescence and ELISA) for anti-DNA antibodies which use cell membrane DNA (cmDNA) as antigen (Wil2 NS lymphoblastoid B cell line).

Methods: We tested 97 sera of patients with systemic lupus erythematosus (SLE) and 140 control sera from healthy subjects or from patients with other systemic autoimmune diseases or infectious diseases. The results obtained with the two anti-cmDNA kits were compared with those obtained with three other methods: indirect immunofluorescence on Crithidia luciliae, ELISA anti-double-stranded DNA (anti-dsDNA) and ELISA anti-nucleosome.

Results: The diagnostic sensitivity was 85% for immunofluorescence cmDNA and 90% for ELISA cmDNA, i.e., much higher than that of Crithidia (51%) and ELISA dsDNA (71%) methods, and the same as that of the ELISA nucleosome test (85%). The specificity of the cmDNA tests proved lower (92% for immunofluorescence and 87% for ELISA) than that found by anti-dsDNA Crithidia (99%), ELISA (99%) and anti-nucleosome tests (100%).

Conclusions: The results of our study indicate that cmDNA tests may be used as screening tests in patients with suspected SLE. The anti-nucleosome test offers the best diagnostic accuracy overall and can play an important role in the serological diagnosis of SLE.

Clin Chem Lab Med 2008;46:458–62.


Corresponding author: Dr. Elio Tonutti, MD, Laboratorio di Immunopatologia e Allergologia, Azienda Ospedaliero-Universitaria S. Maria della Misericordia, P.le S. Maria della Misericordia, 33100 Udine, Italy Phone: +39-0432-552403, Fax: +39-0432-552402,

Received: 2007-9-28
Accepted: 2007-12-17
Published Online: 2008-04-01
Published in Print: 2008-04-01

©2008 by Walter de Gruyter Berlin New York