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Licensed Unlicensed Requires Authentication Published by De Gruyter February 27, 2008

The apolipoprotein A5 gene –1131T→C polymorphism affects vitamin E plasma concentrations in type 2 diabetic patients

Josefa Girona, Montse Guardiola, Anna Cabré, Josep M. Manzanares, Mercedes Heras, Josep Ribalta and Lluís Masana

Abstract

Background: Variations of the apolipoprotein A5 (APOA5) gene are strongly associated with hypertriglyceridemia. Vitamin E is transported in triglyceride (TG)-rich lipoproteins and therefore could also be modulated by apoAV. Patients with type 2 diabetes have a tendency towards high TG values and increased oxidative stress.

Methods: We examined the impact of genetic APOA5 variation (1131TC) on vitamin E and oxidative status in 169 non-smoker type 2 diabetic patients. Plasma samples were analyzed for lipids, lipoproteins, vitamin E, oxidized low-density lipoprotein (oxLDL), lipoperoxides, autoantibodies against oxLDL and diene formation of LDL.

Results: Vitamin E concentrations were higher in TC carriers compared with TT carriers (45.48±8.20  μmol/L vs. 40.32±10.47 μmol/L; p=0.02). The prevalence of the TC genotype was 2.6-fold higher among individuals with high vitamin E concentrations (p=0.02). The APOA5 polymorphism did not determine any differences in oxidative status. Fasting TG concentration was a significant 21% higher in carriers of the TC genotype (p=0.04) due to higher TG concentrations in very-low-density lipoprotein (VLDL) and high-density lipoprotein.

Conclusions: The APOA5–1131TC polymorphism is associated with both higher vitamin E concentrations and higher VLDL-TGs in diabetic patients.

Clin Chem Lab Med 2008;46:453–7.


Corresponding author: Lluís Masana, MD, PhD, Unitat de Recerca en Lípids i Arteriosclerosi, Facultat de Medicina i Ciències de la Salut, C. Sant Llorenç, 21, 43201 Reus, Spain Phone: +34-977759366, Fax: +34-977759322,

Received: 2007-9-20
Accepted: 2008-1-2
Published Online: 2008-02-27
Published in Print: 2008-04-01

©2008 by Walter de Gruyter Berlin New York