Accessible Requires Authentication Published by De Gruyter May 22, 2008

Rapid screening by real-time 16S rDNA PCR for bacterial contamination of blood products

Hendrik W. Reesink, Tamimount Mohammadi, Rubyn N.I. Pietersz and Paul H.M. Savelkoul

Abstract

Although blood component transfusion is currently regarded as safe, adverse events may occur in recipients of these products. Among those, blood borne viral, bacterial and parasitic infections are best known. For detection of bacterial contamination in platelet concentrates various methods are available or under investigation. One of these methods, real-time polymerase chain reaction (PCR) with particular focus on real-time 16S rDNA detection, will be discussed in this review.

Clin Chem Lab Med 2008;46:954–62.


Corresponding author: H.W. Reesink, Sanquin Blood Bank North-West Region and Sanquin Diagnostic Services, P.O. Box 9137, 1006 Amsterdam, The Netherlands, ,

Published Online: 2008-05-22
Published in Print: 2008-07-01

©2008 by Walter de Gruyter Berlin New York