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Licensed Unlicensed Requires Authentication Published by De Gruyter July 7, 2008

Use of the TLX ultracentrifuge for the isolation of different density lipoproteins and effects of freeze/thawing of human plasma before ultracentrifugation

  • Valentine Charlton-Menys , Jelena Chobotova and Paul N. Durrington


Background: Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery.

Methods: We isolated a range of lipoproteins using a preparative ultracentrifuge and the TLX micro-ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand.

Results: In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL. Freeze/thawing had no effect on closely correlated results for small-dense LDL apoB (r=0.85; p<0.0001) or high-density lipoprotein (r=0.93; p<0.0001).

Conclusions: The TLX micro-ultracentrifuge is a reliable alternative to the preparative ultracentrifuge and freeze/thawing has only a small effect on small-dense LDL apoB or high-density lipoprotein cholesterol.

Clin Chem Lab Med 2008;46:1285–8.

Corresponding author: Dr. Valentine Charlton-Menys, PhD, Cardiovascular Research Group, School of Clinical and Laboratory Sciences, Core Technology Facility (3rd Floor), University of Manchester, 46 Grafton Street, Manchester M13 9NT, UK

Received: 2008-3-17
Accepted: 2008-5-8
Published Online: 2008-07-7
Published in Print: 2008-09-01

©2008 by Walter de Gruyter Berlin New York

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