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Licensed Unlicensed Requires Authentication Published by De Gruyter March 19, 2010

Microsatellite polymorphisms in intron 2 of the toll-like receptor 2 gene and their association with susceptibility to pulmonary tuberculosis in Han Chinese

Yun Xue, Lei Jin, A-Zhong Li, Hua-Jun Wang, Meng Li, Yu-Xiang Zhang, Yu Wang and Ji-Cheng Li

Abstract

Background: Toll-like receptor 2 (TLR2) is essential for the immune response to tuberculosis (TB). The goal of the present study was to investigate whether the guanine-thymine (GT) repeat microsatellite polymorphism in intron 2 of the TLR2 gene might be correlated with susceptibility to TB in Han Chinese.

Methods: The number of (GT)n repeats was determined by gene scanning from 244 patients with TB and 233 control subjects. The expression of TLR2 on CD14+ peripheral blood mononuclear cells was determined using flow cytometry.

Results: No association in allelic polymorphism between control subjects and patients with TB was found. However, the S/M genotype of the microsatellite polymorphism was more frequent in TB patients than in healthy controls (p=0.01). The S/L genotype was more popular in controls than in patients with TB (p=0.007). TLR2 expression was higher in subjects with the S/L genotype than in those with the S/M genotype (p<0.05).

Conclusions: Our data suggest that the S/M genotype of the microsatellite (GT)n polymorphisms in intron 2 of the TLR2 gene may increase susceptibility to TB in Chinese, and the S/L genotype may act as a negative risk factor.

Clin Chem Lab Med 2010;48:785–9.


Corresponding author: Ji-Cheng Li, Institute of Cell Biology, Zhejiang University School of Medicine, 388, Yuhangtang Road, Hangzhou 310058, P.R. China Phone: +86 571-88208088, Fax: +86 571-88208094,

Received: 2009-10-1
Accepted: 2010-1-15
Published Online: 2010-03-19
Published in Print: 2010-06-01

©2010 by Walter de Gruyter Berlin New York