Accessible Unlicensed Requires Authentication Published by De Gruyter October 30, 2010

First certified reference material for cystatin C in human serum ERM-DA471/IFCC

Anders Grubb, Søren Blirup-Jensen, Veronica Lindström, Camilla Schmidt, Harald Althaus, Ingrid Zegers and on behalf of the IFCC Working Group on Standardisation of Cystatin C (WG-SCC)

Abstract

The IFCC Working Group for the Standardisation of Cystatin C (WG-SCC), in collaboration with the Institute for Reference Materials and Measurements (IRMM), announces the availability of the new certified reference material ERM-DA471/IFCC. The material was characterised using a pure protein primary reference preparation (PRP) as calibrant. The PRP was prepared from recombinant cystatin C, and its concentration measured using dry mass determination. The characterisation of ERM-DA471/IFCC was performed by particle enhanced immuno-nephelometry, particle enhanced immuno-turbidimetry, and enzyme amplified single radial immuno-diffusion. The certified cystatin C mass concentration in ERM-DA471/IFCC, if reconstituted according to the specified procedure, is 5.48 mg/L, the expanded uncertainty (k=2) being 0.15 mg/L.

Clin Chem Lab Med 2010;48:1619–21.


Corresponding author: Ingrid Zegers, European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, Geel 2440, Belgium Phone: +32-14-571920, Fax: +32-14-571548,

Published Online: 2010-10-30
Published in Print: 2010-11-01

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