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Licensed Unlicensed Requires Authentication Published by De Gruyter August 29, 2011

Rapid and simple IgG specific test for the exclusion of heparin induced thrombocytopenia (HIT)

Hans-Jürgen Kolde, Ralf Dostatni and Susanne Mauracher

Abstract

Background: The exclusion of heparin induced thrombocytopenia (HIT) is required for selecting the most appropriate anticoagulation therapy in affected patients. It requires the combination of clinical data with the detection of antibodies directed against platelet factor 4 (PF4) in complex with polyanions (PA) such as heparin.

Methods: We developed a lateral flow immunoassay (LFIA) for PF4/PA complex specific IgG antibodies based on gold nanoparticles. Unlike most other assays, the initial immune reaction takes place in the liquid phase. The sensitivity of the assay has been adjusted with clinical samples aiming in the reliable detection of sera which are positive in a functional platelet activation assay.

Results: Sera from 60 patients with suspected HIT were investigated. LFIA identified correctly all samples (n=20) which were positive in a functional assay (HIPA) and an IgG specific ELISA. It correlated with ELISA, but false positive results were less frequent (7 samples were negative with LFIA and HIPA but positive with ELISA).

Conclusions: The LFIA may be a suitable tool for the rapid exclusion of HIT within 10 min.


Corresponding author: Hans-Jürgen Kolde, PhD, Consulting Diagnostics, Gartenstr. 4, 85521 Ottobrunn, Germany Phone: +49 89 6098292, Fax: +49 89 6097580

Received: 2011-7-27
Accepted: 2011-8-1
Published Online: 2011-8-29
Published in Print: 2011-12-1

©2011 by Walter de Gruyter Berlin Boston

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