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Accessible Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

UV-microscopic analysis of acetylated spruce and birch cell walls

Christian Hansmann, Manfred Schwanninger, Barbara Stefke, Barbara Hinterstoisser and Wolfgang Gindl
From the journal

Abstract

Spruce and birch earlywood was acetylated to different weight percent gains using three different acetylation procedures. The absorbance spectra of secondary cell wall and compound cell corner middle lamella were determined by means of UV microscopy. Analysis of the spectra showed that the characteristic lignin absorbance peak in the UV spectrum of wood around 280 nm shifted to shorter wavelengths in acetylated samples. A distinct relationship between achieved weight percent gains after acetylation and observed spectral shifts could be established revealing a certain potential to measure acetylation on a cellular level by means of UV microscopy.

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Corresponding author. Institut für Holzforschung, Universität für Bodenkultur, Gregor Mendel Straße 33, A-1180 Wien, Austria. Fax: +43-1-476544295, E-mail:

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Published Online: 2005-06-01
Published in Print: 2004-08-01

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