Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

On-line study of lignin behaviour in dilute alkaline solution by the SEC-UV method

  • Tatjana Bikova , Arnis Treimanis , Galina Rossinska and Galina Telysheva
From the journal Holzforschung


The dynamic method for monitoring ionisation of the lignin functional groups in a 0.1 N NaOH solution at ambient temperature by means of the SEC technique with multiwave UV detection was performed. An increase in absorbance at 210, 278, 304, 320 and 350 nm was examined to reveal the ionisation dynamics in respect to functional groups’ acidity; that is, carboxyl groups, carbonyl- and double-bond conjugated phenol hydroxyls. A distinctive response of absorbance at 210 and 278 nm on the ionisation of carbonyl- and double-bond-conjugated phenols was found. Simultaneous characterisation of lignin by molecular weight (MW) and chemical heterogeneity was performed. A non-uniform distribution of reactive sites over the MW distribution for commercial kraft lignin (Curan) was found employing the family of chemical composition distribution curves. The redistribution of extended chromophore structures over time along the lignin’s MW distribution causes a misrepresentation of the UV chromatogram pattern and influences the accuracy of MW values.


Corresponding author. 27 Dzerbenes Str., Riga, LV-1006, Latvia. Fax: +371-7553063, E-mail:


Aliev, R.G., Burov, A.V., Rusakov, A.E. (1990) Study of the molecular mass characteristics of lignin isolated in the process of organosolv treatment of birch wood. Khimiya drevesiny (Wood Chemistry)3:45–49.Search in Google Scholar

Bikova, T., Treimanis, A. (2002a) Ability of HP SEC method in monitoring of kraft delignification process. Proc. Control Systems 2002, Stockholm, Sweden. pp. 249–252.Search in Google Scholar

Bikova, T., Treimanis, A. (2002b) Multi-wave UV/VIS detection in SEC analysis of lignin and lignin-carbohydrate complex structure. Proc. 7th EWLP, 26–29 August, Turku/Abo, Finland. pp. 209–212.Search in Google Scholar

Bikova, T., Eremeeva, T., Klevinska, V., Treimanis, A. (2000a) An approach to the study of residual lignin in kraft pulp. Proc. 6th EWLP, Bordeaux, France. pp. 227–230.Search in Google Scholar

Bikova, T., Klevinska, V., Treimanis, A. (2000b) Monitoring of lignin and hemicelluloses in spent cooking liquor. Holzforschung54:66–70.10.1515/HF.2000.010Search in Google Scholar

Bikova, T., Klevinska, V., Eremeeva, T., Treimanis, A. (2001) Study on alkali-accessible chromophores from unbleached kraft pulp. Holzforschung55:554–558.10.1515/HF.2001.089Search in Google Scholar

Blazej, A., Šuty, L. Vegetable phenol components. Mir, Moscow, 1977.Search in Google Scholar

Bogolitsyn, K.G., Khabarov, Yu. (1985) UV-spectroscopy of lignin. Review. Khimiya drevesiny (Wood Chemistry)6:3–29.Search in Google Scholar

Bogolitsyn, K.G., Lindberg, J. (1986) The estimation of the OH-acidity of the model compounds of lignin structural link of the derivative UV-spectroscopy. Khimiya drevesiny (Wood Chemistry)4:56–60.Search in Google Scholar

Bogolitsyn, K.G., Reznikov, V.M. Chemistry of sulphite methods of wood delignification. Ecologiya, Moscow, 1994.Search in Google Scholar

Chum, H., Johnson, L., Tucker, M., Himmel, H. (1987) Some aspects of lignin characterisation by high performance size exclusion chromatography using styrene divinylbenzene copolymer gels. Holzforschung41:97–108.10.1515/hfsg.1987.41.2.97Search in Google Scholar

Furman, G.S., Lonsky, W.F.W. (1988) Charge-transfer complexes in kraft lignin. J. Wood Chem. Technol.8:165–208.10.1080/02773818808070678Search in Google Scholar

Gärtner, A., Gellerstedt, G., Tamminen, T. (1999) Determination of phenolic hydroxyl groups in residual lignin using a modified UV-method. Nordic Pulp Paper Res. J.14:163–170.10.3183/npprj-1999-14-02-p163-170Search in Google Scholar

Gellerstedt, G., Lindfors, L. (1984) Structural changes in lignin during kraft cooking. 4. Phenolic hydroxyl groups in wood and kraft pulps. Svensk Papperstidn.15:R115–R118.Search in Google Scholar

Gierer, G., Imsgard, F. (1977) The reactions of lignins with oxygen and hydrogen peroxide in alkaline media. Svensk Papperstidn.80:510–518.Search in Google Scholar

Hortling, B., Kokkonen, P. (2001) Preparative GPC as a tool in characterization of lignocellulosics materials. Proc. Postsymp., 11th Int. Symp. Wood and Pulping Chem., 18–19 June 2001, Grenoble, France. pp. 99–102.Search in Google Scholar

Jakobsons, J., Novikovs, A., Erinš, P. (1992) High temperature steam autohydrolysis of wood components. 3. Gel permeation chromatography of autohydrolysis lignin and pseudolignin. Wood Chemistry6:65–69.Search in Google Scholar

Lin, S.Y. (1982) Derivative ultraviolet spectroscopy of lignin and lignin model compounds – A new analytical technique. Svensk Papperstidn.85:R162–R171.Search in Google Scholar

Mlynar, J., Kolar, J. (1988) Gel chromatography of lignin and lignin model compounds on the Separon HEMA 1000 BIO column. Papir a celuloza43(3):V22–V24.Search in Google Scholar

Nefedov, P.P., Rusakov, A.E., Ivanov, M.A., Scherbakova, A.D., Lazareva, M.A., Zakharov, V.I. (1981) Polyelectrolyte effects during exclusion chromatography of lignin, lignin-carbohydrate complex and polysaccharides. Khimija drevesiny (Wood Chemistry)4:66–72.Search in Google Scholar

Norgen, M., Lindström, B. (2000) Physico-chemical characterization of a fractionated kraft lignin. Holzforschung54:528–534.10.1515/HF.2000.089Search in Google Scholar

Pasco, F., Suckling, I.D. (1998) Chromophore changes during oxygen delignification of a radiata pine kraft pulp. Appita J.52:138–136.Search in Google Scholar

Pellinen, J., Salkinoja-Salonen, M. (1985) Aqueous size exclusion chromatography of industrial lignins. J. Chromatogr.322:129–138.10.1016/S0021-9673(01)97665-4Search in Google Scholar

Sarkanen, S., Teller, D.C., Stevens, C.R., McCarty, J.L. (1984) Lignin. 20. Associative interactions between kraft lignin components. Macromol.17:2588–2597.Search in Google Scholar

Tamminen, T., Sipilä, J. (2001) Determination of phenolic hydroxyl groups by UV-spectroscopy. Proc. Post-symp. 11th Int. Symp. Wood and Pulping Chem., 18–19 June 2001, Grenoble, France. pp. 27–30.Search in Google Scholar

Wong, K.K.Y., de Jong, E. (1996) Size-exclusion chromatography of lignin- and carbohydrate-containing samples using alkaline eluents. J. Chromatogr. A 737:193–203.Search in Google Scholar

Zakis, G.F. Functional analysis of lignins and their derivatives. TAPPI PRESS, Atlanta GA, 1994.Search in Google Scholar

Published Online: 2005-06-01
Published in Print: 2004-08-01

© Walter de Gruyter

Downloaded on 23.9.2023 from
Scroll to top button