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Application of scanning UV microspectrophotometry for the topochemical detection of lignin within individual cell walls of brown-rotted Scots pine (Pinus sylvestris L.) sapwood

Ilze Irbe, Guna Noldt, Gerald Koch, Ingeborga Andersone and Bruno Andersons
From the journal


Brown rotted Scots pine (Pinus sylvestris) sapwood was studied using scanning UV microspectrophotometry. Wood blocks were exposed to the fungus Coniophora puteana (Schum.: Fr.) Karst. (BAM Ebw.15) for 6, 8, 10, 30, and 50 days. No wood weight loss was detected in the initial decay periods. On the other hand, point analyses of lignin distribution in wood cells revealed higher absorbance after 6–10 days of decay, which we interpret as an increase in the absorption coefficient of lignin due to its oxidative modification by the fungus. Uneven wood degradation occurred in the later periods (30 and 50 days), with both significantly decayed and visually sound cells observed. The decayed cells showed a higher absorbance at 280 nm, although the apparently sound cells were also degraded to a lower extent. Degradation of lignin-rich compounds in middle lamellae and cell corners was not observed during fungal attack.


Corresponding author. Laboratory of Wood Biodegradation and Protection, Latvian State Institute of Wood Chemistry, Dzerbenes iela 27, LV-1006, Riga, Latvia


Bauch, J., Seehann, G., Fitzer, H. (1976) Microspectrophotometrical investigations on lignin of decayed wood. Mater. Organism.3:141–152.Search in Google Scholar

Cohen, R., Jensen, K.A. Jr., Houtman, C.J., Hammel, K.E. (2002) Significant levels of extracellular reactive oxygen species produced by brown rot basidiomycetes on cellulose. FEBS Lett.531:483–488.Search in Google Scholar

Cohen, R., Suzuki, M.L., Hammel, K.E. (2005) Processive endoglucanase active in crystalline cellulose hydrolysis by the brown rot basidiomycete Gloeophyllum trabeum. Appl. Environ. Microbiol.71:2412–2417.Search in Google Scholar

Eriksson, K.E., Blanchette, R.A., Ander, P. Microbial and Enzymatic Degradation of Wood and Wood Components. Springer-Verlag, Berlin, 1990.Search in Google Scholar

European Standard (2000) EN 113. Wood preservatives. Test method for determining the protective effectiveness against wood-destroying basidiomycetes.Search in Google Scholar

Fengel, D., Wegener, G. Wood – Chemistry, Ultrastructure, Reactions. Walter de Gruyter, Berlin, 1984.Search in Google Scholar

Flournoy, D.S., Kirk, T.K., Highley, T.L. (1991) Wood decay by brown-rot fungi: Changes in pore structure and cell wall volume. Holzforschung45:383–388.Search in Google Scholar

Fukuzawa, K., Imagawa, H. (1981) Quantitative analysis of lignin using an UV microscopic image analyser. Variation within one growth element. Wood Sci. Technol.15:45–55.Search in Google Scholar

Fukazawa, K. (1992) Ultraviolet microscopy. In: Methods in Lignin Chemistry. Eds. Lin, S.Y., Dence, C.W. Springer-Verlag, Berlin. pp. 110–121.Search in Google Scholar

Goodell, B., Nicholas, D.D., Schultz, T.P. Wood Deterioration and Preservation. American Chemical Society, Washington, DC, 2003.Search in Google Scholar

Green, F., Highley, T.L. (1997) Brown-rot wood decay – Insights gained from a low-decay isolate of Postia placenta. Trends Plant Pathol.1:1–17.Search in Google Scholar

Highley, T.L. (1988) Cellulolytic activity of brown-rot and white-rot fungi on solid media. Holzforschung42:211–216.Search in Google Scholar

Highley, T.L., Dashek, W.V. (1998) Biotechnology in the study of brown- and white rot decay. In: Forest Products, Biotechnology. Eds. Bruce, A., Palfreyman, J.W. T.J. International Ltd., UK. pp. 15–36.Search in Google Scholar

Irbe, I., Andersons, B., Chirkova, J., Kallavus, U., Andersone, I., Faix, O. (2006) On the changes of pinewood (Pinus sylvestris L.) chemical composition and ultrastructure during the attack by brown-rot fungi Postia placenta and Coniophora puteana.Int. Biodeter. Biodegrad.57:99–106.Search in Google Scholar

Jensen, K.A. Jr., Houtman, C.J., Ryan, Z.C., Hammel, K.E. (2001) Pathways for extracellular Fenton chemistry in the brown rot basidiomycete Gloeophyllum trabeum. Appl. Environ. Microbiol.67:2705–2711.Search in Google Scholar

Jensen, W.A. Botanical Histochemistry: Principles and Practice. W.H. Freeman, San Francisco, 1962.Search in Google Scholar

Jin, L., Schultz, T.P., Nicholas, D.D. (1990) Structural characterisation of brown-rotted lignin. Holzforschung44:133–138.Search in Google Scholar

Kirk, T.K. (1975) Effects of a brown-rot fungus, Lenzites trabea, on lignin on spruce wood. Holzforschung29:99–107.Search in Google Scholar

Kleist, G., Schmitt, U. (2001) Characterisation of a soft rot-like decay pattern caused by Coniophora puteana (Schum.) Karst. in Sapelli wood (Entandrophragma cylindricum Sprague). Holzforschung55:573–578.Search in Google Scholar

Kleist, G., Seehann, G. (1997) Colonization patterns and topochemical aspects of sap streak caused by Stereum sanguinolentum in Norway spruce. Eur. J. For. Pathol.27:351–361.Search in Google Scholar

Koch, G., Grünwald, C. (2004) Application of UV microspectrophotometry for the topochemical detection of lignin and phenolic extractives in wood fibre cell walls. In: Wood Fibre Cell Walls: Methods to Study their Formation, Structure and Properties. COST Action E20. pp. 119–130.Search in Google Scholar

Koch, G., Kleist, G. (2001) Application of scanning UV microspectrophotometry to localise lignins and phenolic extractives in plant cell walls. Holzforschung55:563–567.Search in Google Scholar

Kramer, C., Kreisel, G., Fahr, K., Kaessbohrer, J., Schlosser, D. (2004) Degradation of 2-fluorphenol by the brown-rot fungus Gloeophyllum striatum: Evidence for the involvement of extracellular Fenton chemistry. Appl. Microbiol. Biotechnol.64:387–395.Search in Google Scholar

Lee, K.H., Wi, S.G., Singh, A.P., Kim, Y.S. (2004) Micromorphological characteristics of decayed wood and laccase produced by the brown-rot fungus Coniophora puteana. J. Wood Sci.50:281–284.Search in Google Scholar

Martinez, A., Speranza, M., Ruiz-Duenas, F.J., Ferreira, P., Camarero, S., et al. (2005) Biodegradation of lignocellulosics: Microbial, chemical, and enzymatic aspects of the fungal attack of lignin. Int. Microbiol.8:195–204.Search in Google Scholar

Qian, Y., Goodell, B., Felix, C.C. (2002) The effect of low molecular weight chelators on iron chelation and free radical generation as studied by ESR measurements. Chemosphere48:21–28.Search in Google Scholar

Sarkanen, K.V., Hergert, H.L. (1971) Classification and distribution. In: Lignins, Occurrence, Formation, Structure and Reactions. Eds. Sarkanen, K.V., Ludwig, C.H. Wiley Interscience, New York. pp. 43–49.Search in Google Scholar

Schmidt, O., Schmitt, U., Moreth, U., Potsch, T. (1997) Wood decay by the white-rotting basidiomycete Physisporinus vitreus from a cooling tower. Holzforschung51:193–200.Search in Google Scholar

Spurr, A.R. (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res.26:31–43.Search in Google Scholar

Teeri, T.T. (1997) Crystalline cellulose degradation: new insight into the function of cellobiohydrolases. Trends Biotechnol.15:160–167.Search in Google Scholar

Tornberg, K., Olsson, S. (2002) Detection of hydroxyl radicals produced by wood-decomposing fungi. FEMS Microbiol. Ecol.40:13–20.Search in Google Scholar

Yang, W., Liu, J., Wang, W., Zhang, Y., Gao, P. (2004) Function of low molecular peptide generated by cellulolytic fungi for the degradation of native cellulose. Biotechnol. Lett.26:1799–1802.Search in Google Scholar

Varela, E., Tien, M. (2003) Effect of pH and oxalate on hydroquinone-derived hydroxyl radical formation during brown rot wood degradation. Appl. Environ. Microbiol.69:6025–6031.Search in Google Scholar

Published Online: 2006-12-04
Published in Print: 2006-11-01

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