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Licensed Unlicensed Requires Authentication Published by De Gruyter March 27, 2008

Topochemical investigation on tension wood fibres of Acer spp., Fagus sylvatica L. and Quercus robur L.

Christian Lehringer, Notburga Gierlinger and Gerald Koch
From the journal

Abstract

Topochemical detection of lignin and phenolic compounds was carried out at subcellular level in tension wood fibres of maple (Acer spp.), beech (Fagus sylvatica) and oak (Quercus robur) by means of cellular UV-microspectrophotometry (UMSP) and confocal Raman microscopy. UMSP field scans at 278 nm revealed the presence of aromatic compounds in the gelatinous layer (G-layer) in tension wood of all three species. Characterisation of the detected substances by point measurements from 240 nm to 400 nm revealed a plausible relationship to by-products from the lignin biosynthesis pathway. Raman spectra, recorded from different regions within the G-layer, showed a high affinity to spectra of lignins. The degree of aromatic compounds in the G-layer of oak tension wood fibres was approximately three-fold higher than that in maple and beech. Moreover, the oak G-layer showed an accumulation of aromatic compounds towards the cell lumen up to 50% higher than that detected in the secondary cell wall. UMSP and Raman microscopy are considered valuable complementary methods for topochemical investigation on a subcellular level.


Corresponding author. Institute for Wood Biology and Wood Protection, Federal Research Centre for Forestry and Forest Products, Leuschnerstrasse 91, 21031 Hamburg, Germany

Received: 2007-8-7
Accepted: 2007-10-17
Published Online: 2008-03-27
Published Online: 2008-12-10
Published in Print: 2008-05-01

©2008 by Walter de Gruyter Berlin New York