Accessible Unlicensed Requires Authentication Published by De Gruyter August 11, 2011

Identification and functional characterization of a sesquiterpene synthase gene from Eleutherococcus trifoliatus

Chi-Hsiang Wen, Yen-Hsueh Tseng and Fang-Hua Chu
From the journal

Abstract

In the present study, one sesquiterpene synthase gene in Eleutherococcustrifoliatus was identified and characterized. Full-length cDNA was obtained from stems. It contained an open reading frame of 1671 bp (EtCop) with a predicted molecular mass of 64.5 kDa. The amino acid sequence of EtCop contained the common terpene synthase family motifs RR(x)8W, RxR and DDxxD. The recombinant protein from Escherichia coli was incubated with farnesyl diphosphate in order to identify the function of EtCop. The product of EtCop could be identified as an α-copaene by means of gas chromatography-mass spectrometry analysis and comparison with an authentic standard.


Corresponding author. School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan Phone: +886-2-33665261 Fax: +886-2-23654520

Received: 2011-4-11
Accepted: 2011-7-7
Published Online: 2011-08-11
Published in Print: 2012-02-01

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