Accessible Requires Authentication Published by De Gruyter March 17, 2009

Does progesterone inhibit bacteria-stimulated interleukin-8 production by lower genital tract epithelial cells?

Morgan R. Peltier, Yana Berlin, Siew C. Tee and John C. Smulian
From the journal


Objective: Progesterone (P4) has been clinically shown to prevent the recurrence of preterm birth. The mechanism(s) of action is unclear, but may involve modulation of the immunologic inflammatory response of the lower genital tract. We evaluated the effects of P4 on interleukin-8 (IL-8) production by vaginal and cervical epithelial cells stimulated with bacterial species that are commonly associated with preterm birth.

Methods: Vaginal and endocervical epithelial cells were incubated with up to 10,000 ng/mL P4 overnight and stimulated with heat-killed Escherichia coli,Gardnerella vaginalis, or Ureaplasma urealyticum. Concentrations of IL-8 in conditioned medium were quantified by ELISA and viability of the cell cultures was measured by the reduction of a tetrazolium salt.

Results:E. coli, G. vaginalis and U. urealyticum-stimulated IL-8 production for both cell lines. P4 inhibited basal and bacteria-stimulated IL-8 production for vaginal epithelial cells but enhanced IL-8 production by endocervical cells. P4 reduced the number of viable cells for both cell lines.

Conclusions: P4 inhibits IL-8 production by vaginal epithelial cells stimulated with pathogens associated with preterm birth, possibly by reducing the number of viable cells or by inhibiting their proliferation. Although P4 also reduces proliferation of endocervical cells it also increases their production of IL-8.

Corresponding author: Morgan R. Peltier, PhD Perinatal Research Laboratory Applied Bench Core Winthrop University Hospital Mineola, NY 11501 USA Tel.: +1 (516) 663 2035 Fax: +1 (516) 663-8871

Received: 2008-9-30
Revised: 2008-11-24
Accepted: 2008-11-27
Published Online: 2009-03-17
Published Online: 2009-03-17
Published in Print: 2009-07-01

©2009 by Walter de Gruyter Berlin New York