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Licensed Unlicensed Requires Authentication Published by De Gruyter December 30, 2014

Serological screening of selected microsporidia in HPV-positive women

Jozef Adam, Alexandra Valenčáková, Monika Halánová, Oľga Danišová, Katarína Gorbarová and Lýdia Čisláková
From the journal Acta Parasitologica


Microsporidiosis is considered to be emerging opportunistic infection in immunocompromised individuals worldwide. The aim of this study was to identify the specific serum antibodies to intestinal microsporidia Encephalitozoon cuniculi and Encephalitozoon intestinalis in women with Human Papillomavirus HPV and without HPV by the indirect immunofluorescence (IFA). From total number of 669 examined women, 225 were HPV positive and 444 women HPV negative. Overall the study comprised of 10.8% women with positive result for presence of E. cuniculi antibodies. In group 1 (HPV-positive women) it was more than 28% and in group 2 (HPV-negative women) it was less than 2% (p<0.001). E. intestinalis infection was found in total of 4.48% women, in group 1 it was present in less than 6% and in group 2 in less than 4% of women.


Dascomb K., Frazer T., Clark R.A., Kissinger P., Didier E., 2000. Microsporidiosis and HIV. Journal of Acquired Immune Deficienty Syndromes, 24, 290-292Search in Google Scholar

Didier P., Shadduck J. A. 1998. Modulated immune responsiveness associated with experimental Encephalitozoon cuniculi infection in BALB/c mice. Laboraory Animal Science, 38, 680-684Search in Google Scholar

Franka R., Švrček Š., Suli J., Beníšek Z., Maďar M., Hipíková V., Levkutová M., Závadová J., Ondrejková A., Ondrejka R., Levkut M., Révajová V. 2006. Influence of Encephalitozoon cuniculi experimental infection on the antirabies immunity. Bulletin of the Veterinary Institute in Pulawy. 50, 309-313 Search in Google Scholar

Gissman L. 1997. Immunologic responses to human papillomavirus infection. Obstetrics Gynecology, 90, 7-11 Search in Google Scholar

Chalupský J., Vávra J., Bedrník P. 1973. Detection of antibodies to Encephalitozoon cuniculi in rabbits by the indirect immunofluorescent antibody test. Folia Parasitologica, 20, 281-284Search in Google Scholar

Levkutová M., Revajová V., Levkut M. 1998. Subpopulations of lymphocytes in cattle naturally infected with papillomavirus. Acta Veterinaria Hungarica, 46, 13-18Search in Google Scholar

Mohanty K.C., Roy R.V. 1984. Thymus derived lymphocyte (T-cells) in patients with genital warts. Br. Journal of Veneral Disease, 60, 186-188Search in Google Scholar

Moretto M., Durrel B., Khan A.I., Casicotti L. 2000. Lack of CD4+ T cells does not affect induction of CD8+ T cell immunity against Encephalitozoon cuniculi infection, Infection & Immunity, 68, 6223-6232Search in Google Scholar

Moretto M., Weiss L.M., Khan I.A. 2004. Induction of a rapid and strong antigen-specific intraepithelial lymphocyte response during oral Encephalitozoon cuniculi infection, Journal of Immunology, 172, 4402-4409Search in Google Scholar

Orenstein J.M. 2003. Diagnostic pathology of microsporidiosis. Ultrastructural Pathology, 27, 141-149Search in Google Scholar

Shearer G.M., Berstein C.D., Tung S.K., Via C.S., Redfield R., Salahuddin S.Z., Gallo R.C. 1986. A model for selective loss of major histocompatibility complex self restricted T cell immune response during the development of acquired immune deficiency syndrome (AIDS). Journal of Immunology, 137, 2514-2521Search in Google Scholar

Valencakova A., Balent P., Malcekova B., Lesnik F., Halanova M., Hipikova V. 2002. Comparison of mammalian microsporidia species replication in various cell lines. Biologia, 57, 773-776Search in Google Scholar

Valencakova A., Revajova V., Bálent P., Lešník F., Levkut M. 2003. Immunosuppressive effect of Encephalitozoon cuniculi. Bulletin of the Veterinary Institute in Pulawy. 47, 113-120Search in Google Scholar

Received: 2014-6-2
Revised: 2014-7-15
Accepted: 2014-9-2
Published Online: 2014-12-30
Published in Print: 2014-3-1

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