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Licensed Unlicensed Requires Authentication Published by De Gruyter March 25, 2015

Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces

  • Silvana Carnevale EMAIL logo , María Laura Pantano , Laura Kamenetzky , Jorge Bruno Malandrini , Claudia Cecilia Soria and Jorge Néstor Velásquez
From the journal Acta Parasitologica


Fasciolosis is an important parasitic zoonosis considered the most important helminth infection of ruminants in tropical countries. The aim of this study was to develop a PCR assay for the sensitive and specific detection of F. hepatica in formalin preserved sheep faeces. A 405-bp fragment of the cytochrome c oxidase subunit 1 gene of F. hepatica was amplified from stool samples of infected sheep. The PCR assay showed a detection limit of 20 pg of F. hepatica DNA. No cross-reactions were observed with samples containing coccidian oocysts or gastrointestinal nematodes eggs. Our PCR technique showed to be effective for specific detection of F. hepatica infections in sheep.


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Received: 2014-5-28
Revised: 2014-10-26
Accepted: 2014-11-28
Published Online: 2015-3-25
Published in Print: 2015-6-1

© 2015 W. Stefański Institute of Parasitology, PAS

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