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Licensed Unlicensed Requires Authentication Published by De Gruyter March 25, 2015

Preliminary analysis of Plasmodium vivax genotypes isolated in southeastern Turkey

Aysu Değirmenci Döşkaya, Mert Döşkaya, Ayşe Caner, Kadri Gül, Şebnem Nergiz, Hüseyin Can and Yüksel Gürüz
From the journal Acta Parasitologica

Abstract

Plasmodium vivax is the most common cause of malaria worldwide as well as southeastern Turkey. After the implementation of a successful national elimination program that the local malaria cases were not reported in 2011, malaria returned to county of Savur located in southeastern Turkey in summer of 2012. The present study aimed to determine the prevalent P. vivax genotypes isolated from southeastern Turkey. Genetic polymorphism in P. vivax CSP gene was analyzed by PCR-RFLP to assess the ratio of VK210 and VK247 types. Blood samples were obtained from 15 patients who lived in southeastern between 2005-2006. According to the results, VK210 type was detected in 10 samples (66.6%), VK247 type was observed in three samples (20%). Remaining two samples showed mixed infection (13.3%). The results of the present study first time showed the ratio of P. vivax genotypes in southeastern Turkey before the elimination in 2011. The results of the present study will be enable researchers to compare the new isolates with the previously detected ones and design new treatment and/elimination strategies.

References

Arnot D.E., Barnwell J.W., Tam J.P., Nussenzweig V., Nussenzweig R.S., Enea V. 1985. Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope. Science, 230, 815-818 Search in Google Scholar

de Souza-Neiras W.C., de Melo L.M., Machado R.L.. 2007. The genetic diversity of Plasmodium vivax-a review. Memórias do Instituto Oswaldo Cruz, 102, 245-254Search in Google Scholar

Dias S., Wickramarachchi T., Sahabandu I., Escalante A.A., Udagama P.V. 2013. Population genetic structure of the Plasmodium vivax circumsporozoite protein (Pvcsp) in Sri Lanka. Gene, 518, 381-387Search in Google Scholar

Hernández-Martínez M.Á., Escalante A.A., Arévalo-Herrera M., Herrera S. 2011. Antigenic diversity of the Plasmodium vivax circumsporozoite protein in parasite isolates of Western Colombia. The American Journal of Tropical Medicine and Hygiene, 84, 51-57.Search in Google Scholar

Imwong M., Pukrittayakamee S., Grüner A.C., Rénia L., Letourneur F., Looareesuwan S., White N.J., Snounou G. 2005. Practical PCR genotyping protocols for Plasmodium vivax using Pvcs and Pvmsp1. Malaria Journal, 4, 20 Search in Google Scholar

Inspection and evaluation report of malaria outbreak occurred in Savur, Mardin, 2012. Turkish Medical Association publications.Search in Google Scholar

Kain K.C., Brown A.E., Webster H.K., Wirtz R.A., Keystone J.S., Rodriguez M.H., Kinahan J., Rowland M., Lanar D.E. 1992. Circumsporozoite genotyping of global isolates of Plasmodium vivax from dried blood specimens. Journal of Clinical Microbiology, 30, 1863-1866Search in Google Scholar

Leclerc M.C., Menegon M., Cligny A., Noyer J.L., Mammadov S., Aliyev N., Gasimov E., Majori G., Severini C. 2004. Genetic diversity of Plasmodium vivax isolates from Azerbaijan. Malaria Journal, 3, 40Search in Google Scholar

Lopez A.C., Ortiz A., Coello J., Sosa-Ochoa W., Torres R.E., Banegas E.I., Jovel I., Fontecha G.A. 2012. Genetic diversity of Plasmodium vivax and Plasmodium falciparum in Honduras. Malaria Journal, 11, 391Search in Google Scholar

Machado R.L., Póvoa M.M. 2000. Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment. Transactions of the Royal Society of Tropical Medicine and Hygiene, 94, 377-381Search in Google Scholar

Malaria Surveillance Directorate statistics about Malaria, Ministry of Health, Republic of Turkey, 2014. http://www.saglik.gov.tr/TR/belge/1-3416/sitma-savas-daire-baskanliginin-sitma-ileilgili-istati-.html. Accessed November 18, 2014Search in Google Scholar

Rosenberg R., Wirtz R.A., Lanar D.E., Sattabongkot J., Hall T., Waters A.P., Prasittisuk C. 1989. Circumsporozoite protein heterogeneity in the human malaria parasite Plasmodium vivax. Science, 245, 973-976 Search in Google Scholar

World Health Organization, 2012. Plasmodium vivax Control & Elimination: Development of Global Strategy and Investment Case, Malaria Policy Advisory Committee Meeting, http://www.who.int/malaria/mpac/sep2012/pvivax_development_global_strategy_and_investment_sep2012.pdf. Accessed November 18, 2014Search in Google Scholar

World Health Organization, 2014. World Malaria Report 2012 http://www.who.int/malaria/publications/world_malaria_report_2012/en/index.html. Accessed November 18, 2014Search in Google Scholar

Zakeri S., Abouie Mehrizi A., Djadid N.D., Snounou G. 2006. Circumsporozoite protein gene diversity among temperate and tropical Plasmodium vivax isolates from Iran. Tropical Medicine & International Health, 11, 729-737Search in Google Scholar

Zakeri S., Raeisi A., Afsharpad M., Kakar Q., Ghasemi F., Atta H., Zamani G., Memon M.S., Salehi M., Djadid N.D. 2010b. Molecular characterization of Plasmodium vivax clinical isolates in Pakistan and Iran using pvmsp-1, pvmsp-3alpha and pvcsp genes as molecular markers. Parasitology International, 59, 15-21Search in Google Scholar

Zakeri S., Safi N., Afsharpad M., Butt W., Ghasemi F., Mehrizi A.A., Atta H., Zamani G., Djadid N.D. 2010a. Genetic structure of Plasmodium vivax isolates from two malaria endemic areas in Afghanistan. Acta Tropica, 113, 12-19 Search in Google Scholar

Received: 2014-3-17
Revised: 2014-11-18
Accepted: 2014-12-5
Published Online: 2015-3-25
Published in Print: 2015-6-1

© 2015 W. Stefański Institute of Parasitology, PAS