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Licensed Unlicensed Requires Authentication Published by De Gruyter March 25, 2015

The lack of effectiveness of hyperbaric oxygenation as a treatment for Leishmania major in a mouse model

Ayelet Livneh, Ilan Youngster, Yossef El-On, Matitiahu Berkovitch and Ibrahim Abu-Kishk
From the journal Acta Parasitologica

Abstract

We aimed to study the effectiveness of hyperbaric oxygen therapy (HOT) (100% oxygen at 2 ATA for 70 minutes each session for 20 consecutive days) on BALB/c male mice infected with Leishmania major. Fifty-one mice were assigned to six groups. Group 1 was treated with HOT from 1 day after the inoculation. In Groups 2-5, treatment began when the cutaneous lesions appeared. Group 2 received HOT only, Group 3 received topical therapy with Leshcutan only, Groups 4 and 5 received a combination of HOT and Leshcutan for 5 and 10 days respectively, and Group 6 did not receive any treatment (control group). When comparing the control group with Group 1, treatment with HOT in Group 1 did not significantly affect the time of the appearance of the lesions. In contrast, mice treated with Leshcutan demonstrated a significant difference in lesion size and spleen dimensions as compared to the rest of the mice (p<0.001). The results show that HOT treatment has no positive effect on the course of Leishmaniasis in a BALB/c mice model infected with Leishmania major. Further studies are needed with a mouse model closer to humans and with different HOT protocols.

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Received: 2014-5-25
Revised: 2014-11-20
Accepted: 2014-12-1
Published Online: 2015-3-25
Published in Print: 2015-6-1

© 2015 W. Stefański Institute of Parasitology, PAS