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A new Cosmocercoides species (Nematoda: Cosmocercidae), C. tonkinensis n. sp., in the scale-bellied tree lizard (Acanthosaura lepidogaster) from Vietnam

Binh Thi Tran, Hiroshi Sato and Pham Van Luc
From the journal Acta Parasitologica


A new cosmocercid nematode species, Cosmocercoides tonkinensis n. sp., is described from the scale-bellied tree lizard (Acanthosaura lepidogaster) in the northern and central parts of Vietnam. The new species is characterized by medium-sized male worms (4.2-5.1 mm in length and 0.34-0.37 mm in width) relative to known members of the genus, with lateral alae, two sharply pointed spicules of equal length (0.22-0.26 mm in length), a gubernaculum (0.113-0.122 mm in length), 16 or 17 pairs of caudal rosettes, and the presence of somatic papillae. Female worms are slightly larger than male worms (5.3-5.5 mm in length and 0.32-0.42 mm in width), with the vulva situated at 3/5 from the anterior end, and elliptical embryonated eggs, 0.064- 0.084 mm long by 0.040-0.048 mm wide. From 19 recorded species of the genus, the morphology of C. tonkinensis n. sp. is closest to C. multipapillata, C. bufonis, and C. pulcher reported from toads and frogs in East Asia. The present new species is differentiated from them by the number of caudal rosettes, tail length relative to body length, presence of somatic papillae and lateral alae, and embryonated eggs. Furthermore, after C. variabilis in North America and C. sauria in Brazil, this new species is only the third species to be recorded from a reptilian host. The 18S ribosomal RNA gene (rDNA) of the new species is almost identical to that of C. dukae infecting land snails and slugs in North America. Between the present new species and C. pulcher from a toad (Bufo japonicus) in Japan, remarkably fewer nucleotide changes were noticed in the 18S to 28S rDNA including the internal transcribed spacer regions. The molecular phylogenetic position of the genus Cosmocercoides is briefly discussed.


Ananjeva N.B., Orlov N.L., Kalyabina-Hauf, S.A. 2008. Species of Acanthosaura Gray, 1831 (Agamidae: Sauria. Reptilia) of Vietnam: Results of molecular and morphological study. Biology Bulletin, 35, 178-18610.1134/S106235900802012XSearch in Google Scholar

Anisimova M., Gascuel O. 2006. Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. Systematic Biology, 55, 539-55210.1080/10635150600755453Search in Google Scholar

Ávila R.W., Strussmann C., Da Silva R.J. 2010. A new species of Cosmocercoides (Nematoda: Cosmocercidae) from a gymnophthalmid lizard of western Brazil. Journal of Parasitology, 96, 558-56010.1645/GE-2336.1Search in Google Scholar

Bui T.D., Bursey C.R., Goldberg S.R. 2009. A new species of Thelandros (Nematoda, Oxyuroidea, Pharyngodonidae) in Leiolepis reevesi (Sauria, Agamidae) from Vietnam. Acta Parasitologica, 54, 151-15310.2478/s11686-009-0019-1Search in Google Scholar

Bursey C.R., Goldberg S.R., Telford S.R., Jr. 2007. Gastrointestinal helminths of 14 species of lizards from Panama with descriptions of 5 new species. Comparative Parasitology, 74, 108-14010.1654/4228.1Search in Google Scholar

Chabaud A.G. 1978. Keys to genera of the superfamilies Cosmocercoidea, Seuatoidea, Heterakoidea and Subuluroidea. No. 6. In: (Eds. R. C. Anderson, A. G. Chabaud, and S. Willmott) CIH keys to the Nematode Parasites of Vertebrates. Commonwealth Agricultural Bureaux, Buckinghamshire, U.K., pp. 71Search in Google Scholar

Dereeper A., Guignon V., Blanc G., Audic S., Buffet S., Chevenet F., Dufayard J.F., Guindon S., Lefort V., Lescot M., Claverie J.- M., Gascuel O. 2008. robust phylogenetic analysis for the non-specialist. Nucleic Acids Research, 36, 465-46910.1093/nar/gkn180Search in Google Scholar

Goldberg S.R., Bursey C.R. 2005. Microhyla heymonsi (Dark-sided Chorus Frog). Endoparasites. Herpetological Review, 36, 303Search in Google Scholar

Guindon S., Gascuel O. 2003. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52, 696-70410.1080/10635150390235520Search in Google Scholar

Hasegawa H. 1984. Helminth fauna of five Okinawa amphibian species. Biological Magazine, Okinawa, 22, 11-22 (in Japanese with English summary) Hasegawa H., Ikeda Y. 2007. Paracosmocercella rosettae n. gen. et n. sp. (Nematoda: Cosmocercoidea: Cosmocercidae) collected from the Japanese tree frog, Hyla japonica (Anura: Hylidae), in Japan. Journal of Parasitology, 93, 884-88810.1645/GE-1134R.1Search in Google Scholar

Hsü H.F. 1933. On some parasitic nematodes collected in China. Parasitology, 24, 512-54110.1017/S0031182000020904Search in Google Scholar

Hunt D.J., Spiridonov S.E. 2001. A new genus of Carnoyidae (Nematoda: Rhigonematida) with description of Cattiena trachelomegali gen. n., sp. n. and C. trigoniuli gen. n., sp. n., parasites of spirobolid (Diplopoda) from Vietnam. Nematology, 3, 559-57110.1163/156854101753389176Search in Google Scholar

Hunt D.J., Luc P.V., Spiridonov S.E. 2002. Bizarre Ichthyocephalidae (Nematoda: Rhigonematida) from Vietnam with description of Ichthyocephaloides comatus sp. n., Xystrongnathus priscus gen. n., sp. n. and X. phrissus gen. n., sp. n. Nematology, 4, 829-84310.1163/156854102760402603Search in Google Scholar

Karve J.N. 1944. On a small collection of parasitic nematodes from Anura. Proceedings of the Indian Academy of Science, section B, 19, 71-7710.1007/BF03049773Search in Google Scholar

Khera S. 1958. On a new species of Cosmocercoides. Indian Journal of Helminthology, 10, 6-12Search in Google Scholar

Makouloutou P., Setsuda A., Yokoyama M., Tsuji T., Saita E., Torii H., Kaneshiro Y., Sasaki M., Maeda K., Une Y., Hasegawa H., Sato H. 2013. Genetic variation of Gongylonema pulchrum from wild animals and cattle in Japan based on ribosomal RNA and mitochondrial cytochrome c oxidase subunit I genes. Journal of Helminthology, 87, 326-33510.1017/S0022149X12000442Search in Google Scholar

Malysheva S.V., Luc P.V. 2012. Cattiena fansipanis n. sp. (Nematoda: Rhigonematida: Carnoyidae) from a millipede (Myriapoda: Diplopoda: Spirobolida) in North Vietnam. Systematic Parasitology, 81, 135-14610.1007/s11230-011-9333-8Search in Google Scholar

Malysheva S.V., Spiridonov S.E. 2010. Four new species of Heth Cobb, 1898 (Rhigonematida: Hethidae) from Vietnamese diplopods. Nematology, 12, 567-58510.1163/138855409X126018972397911Search in Google Scholar

Nguyen Q.T. 2006. Herpetological collaboration in Vietnam. In: (Eds. Vences M., J. Kohler J., Ziegler T., Bohme W.), Herpetologia Bonnensis II. Proceedings of the 13th Congress of the Societas Europaea Herpetologica, pp. 233-240Search in Google Scholar

Nguyen V.S., Ho T.C., Nguyen Q.T. 2009. Herpetofauna of Vietnam. Edition of Chimaira, Frankfurt am Main, Hessen, Germany, pp.768Search in Google Scholar

Ramallo G., Bursey C.R., Goldberg S.R. 2007. Two new species of cosmocercids (Ascaridida) in the toad Chaunus arenarum (Anura: Bufonidae) from Argentina. Journal of Parasitology, 93, 910-91610.1645/GE-1131R.1Search in Google Scholar

Rizvi A.N. 2009. Two new species of amphibian nematodes from Bhadra Wildlife Sanctuary, Western Ghats, India. Zootaxa, 2013, 58-6810.11646/zootaxa.2013.1.6Search in Google Scholar

Smythe A.B., Sanderson M.J., Nadler S.A. 2006. Nematode small subunit phylogeny correlates with alignment parameters. Systematic Biology, 55, 972-99210.1080/10635150601089001Search in Google Scholar

Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673-468010.1093/nar/22.22.4673Search in Google Scholar

Tran T.B., Bursey C.R., Goldberg S.R. 2007. Two new species of Pharyngodonidae (Nematoda, Oxyuroidea) in Gekko ulikovskii from Vietnam. Acta Parasitologica, 52, 363-36710.2478/s11686-007-0045-9Search in Google Scholar

Vanderburgh D.J., Anderson R.C. 1986. The relationship between nematodes of the genus Cosmocercoides Wilkie, 1930 (Nematoda: Cosmocercoidea) in toads (Bufo americanus) and slugs (Deroceras leave). Canadian Journal of Zoology, 65, 1650-166110.1139/z87-254Search in Google Scholar

Vanderburgh D.J., Anderson R.C. 1987. Seasonal changes in prevalence and intensity of Cosmocercoides dukae (Nematoda: Cosmocercoidea) in Deroceras leave (Mollusca). Canadian Journal of Zoology, 65, 1662-166510.1139/z87-255Search in Google Scholar

Wilkie J.S. 1930. Some parasitic nematodes from Japan. Annals and Magazine of Natural History, London, 10, 606-61410.1080/00222933008673260Search in Google Scholar

Wood P.L., Jr., Grismer J.L., Grismer L.L., Norhayati A., Chan K.O., Bauer A.M. 2009. Two new montane species of Acanthosaura Gray, 1831 (Squamata: Agamidae) from Peninsular Malaysia. Zootaxa, 2012, 28-4610.11646/zootaxa.2012.1.2Search in Google Scholar

Wood P.L., Jr., Grismer L.L., Grismer J.L., Neang T., Chav T., Holden J. 2010. A new cryptic species of Acanthosaura Gray, 1831 (Squamata: Agamidae) from Thailand and Cambodia. Zootaxa, 2488, 22-3810.11646/zootaxa.2488.1.2Search in Google Scholar

Yamaguti S. 1941. Studies on the helminth fauna of Japan. Pt. 34. Amphibian nematodes, II. Japanese Journal of Zoology, 9, 397-408Search in Google Scholar

Received: 2014-4-7
Revised: 2015-1-8
Accepted: 2015-2-25
Published Online: 2015-6-16
Published in Print: 2015-9-1

© W. Stefański Institute of Parasitology, PAS