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Licensed Unlicensed Requires Authentication Published by De Gruyter June 16, 2015

Supplementary data of Henneguya leporinicola (Myxozoa, Myxosporea) a parasite of Leporinus macrocephalus from fish farms in the state of São Paulo, Brazil

  • Kassia Roberta Hygino Capodifoglio EMAIL logo , Edson Aparecido Adriano , Marcia Ramos Monteiro da Silva and Antônio Augusto Mendes Maia
From the journal Acta Parasitologica


Henneguya leporinicola is a parasite of the gill filament of Leporinus macrocephalus, a characiform fish belonging to the Anostomidae family, which is of major economic importance. Despite the damage it causes in fish, little is known about this parasite. Therefore, a study was undertaken with fourteen specimens of L. macrocephalus taken from fish farms in the state of Sao Paulo. The fish were collected and examined searching for lesions and/or myxosporean plasmodia. One of the specimens (7.14%) contained white elongated plasmodia in the gill filament. The mature spores had elongated bodies with polar capsules of equal size and a caudal length greater than body length. Morphological characteristics identified the parasite as H. leporinicola. Molecular analysis of the 18S rDNA sequence resulted in a 1954 bp, demonstrating significant genetic differences with previously described species of Henneguya/Myxobolus. Phylogenetic analysis comparing the 18S rDNA sequence of H. leporinicola with other species, previously described in South America, and the 20 closest species as indicated by BLASTn Max Score showed H. leporinicola as a basal branch of a subclade composed by Henneguya spp. parasite of characiform hosts.


Adriano E.A., Arana S., Ceccarelli O.S., Cordeiro N.S. 2002. Light and scanning electron microscopy of Myxobolus porofilus n. sp. (Myxosporea: Myxobolidae) infecting the visceral cavity of Prochilodus lineatus (Pisces: Characiforms; Prochilodontidae) cultivated in Brazil. Folia Parasitologica, 49, 259-26210.14411/fp.2002.049Search in Google Scholar PubMed

Adriano E.A., Arana S., Cordeiro N.S. 2005. Histology, ultrastructure and prevalence of Henneguya piaractus (Myxosporea) infecting the gills of Piaractus mesopotamicus (Characidae) cultivated in Brazil. Diseases Aquatic Organisms, 64, 229-35. DOI: 0014Search in Google Scholar

Adriano E.A., Carriero M.M., Maia A.A.M., Silva M.R.M., Naldoni J., Ceccarelli P.S., Arana S. 2012. Phylogenetic and host-parasite relationship analysis of Henneguya multiplasmodialis n. sp. infecting Pseudoplatystoma spp. in Brazilian Pantanal wetland. Veterinary Parasitology, 185, 110-120. DOI: 10.1016/j.vetpar.2011.10.00810.1016/j.vetpar.2011.10.008Search in Google Scholar PubMed

Barta J.R., Martin D.S., Liberator P.A., Dashkevicz M., Anderson J.W., Feighner S.D. et al. 1997. Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. Journal of Parasitology, 83, 262-271. DOI: 10.2307/328445310.2307/3284453Search in Google Scholar

Carriero M.M., Adriano E.A., Silva M.R.M., Ceccarelli O.S., Maia A.A.M. 2013. Molecular phylogeny of the Myxobolus and Henneguya genera with several new South American species. PLos ONE, 8, e73713. DOI:10.1371/journal.pone.007371310.1371/journal.pone.0073713Search in Google Scholar PubMed PubMed Central

Eiras J.C. 2002. Synopsis of the species of the genus Henneguya Thelohan, 1892 (Myxozoa: Myxosporea: Myxobolidae. Systematic Parasitology, 52, 43-5410.1023/A:1015016312195Search in Google Scholar

Eiras J.C., Adriano E.A. 2012. Checklist of the species of the genus Henneguya Thelohan, 1892 (Myxozoa, Myxosporea, Myxobolidae) described between 2002 and 2012. Systematic Parasitology, 83, 95-104. DOI: 10.1007/s11230-012-9374-710.1007/s11230-012-9374-7Search in Google Scholar PubMed

Froese R., Pauly D. FishBase. Disponivel em: <> Access: May 2014Search in Google Scholar

Furuya W.M. Especies nativas. In: MOREIRA, H.L.M. et al. Fundamentos da moderna aquicultura. Canoas: ULBRA 2001 Cap., 10, 83-90Search in Google Scholar

Guindon S., Dufayard J.F., Lefort V., Anisimova M., Hordijk W., Gascuel O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59, 307-321. DOI: 10. 1093/sysbio/syq01010.1093/sysbio/syq010Search in Google Scholar PubMed

Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98Search in Google Scholar

Hallet S.L., Diamant A. 2001. Ultrastructure and small-subunit ribosomal DNA sequence of Henneguya lesteri n sp. (Myxosporea), a parasite of sand whiting Sillago analis (Sillaginidae) from the coast of Queensland, Australia. Diseases of Aquatic Organisms, 46, 197-212. DOI: 10.3354/dao04619710.3354/dao046197Search in Google Scholar PubMed

Kent M.L., Andree K.B., Bartholomew J.L., El-Matbouli M., Desser S.S., Devlin R.H. et al. 2001. Recent advances in our knowledge of the Myxozoa. Journal Eukaryotic Microbiology, 48, 395-413. DOI: tb00173.x Lom J., Arthur J.R. 1989. A guideline for the preparation of species description in Myxosporea. Journal of Fish Diseases, 12, 151-156. DOI: 10.1111/j.1365-2761.1989.tb00287.x 10.1111/j.1365-2761.1989.tb00287.xSearch in Google Scholar

Lom J., Dykova I. 2006. Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitologica, 53, 1, 1-3610.14411/fp.2006.001Search in Google Scholar

Martins M.L., Souza V.N., Moraes J.R.E., Moraes F.R. 1999. Gill infection of Leporinus macrocephalus Garavello et Britski, 1988 (Osteichthyes: Anostomidae) by Henneguya leporinicola n. sp. (Myxozoa: Myxobolidae). Description, Histopathology and Treatment. Revista Brasileira de Biologia, 59, 527-53410.1590/S0034-71081999000300018Search in Google Scholar

Molnár K., Eszterbauer E., Sczekely C., Benko M., Harrach B. 2002. Morphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish. Journal of Fish Diseases, 25, 643-652. DOI: 10.1046/j.1365-2761.2002.00409.x Moreira G.S.A., Adriano E.A., Silva M.R.M., Ceccarelli O.S., Maia A..AM. 2013. Morphology and 18S rDNA sequencing identifies Henneguya visibilis n. sp., a parasite of Leporinus obtusidens from Mogi Guacu River, Brazil. Parasitology Research, 113, 81-90. DOI 10.1007/s00436-013-3629-310.1007/s00436-013-3629-3Search in Google Scholar PubMed

Moreira G.S.A., Adriano E.A., Silva M.R.M., Ceccarelli P.S., Maia A.A.M. 2014. The morphological and molecular characterization of Henneguya rotunda n. sp., a parasite of the gill arch and fins of Salminus brasiliensis from the Mogi Guacu River, Brazil. Parasitology Research, 113, p. 1703-1711. DOI: 10.1007/s00436-014-3815-y Naldoni J., Arana S., Maia A.A.M., Ceccarelli O.S., Tavares L.E.R., Borges F.A. et al. 2009. Henneguya pseudoplatystoma n. sp. causing reduction in epithelial area of gills in the farmed pintado, a South American catfish: Histopathology and Ultrastructure. Veterinary Parasitology, 66, 52-59. DOI:10. 1016/j.vetpar.2009.07.034Search in Google Scholar

Naldoni J., Maia A.A.M., Silva M.R.M., Carriero M.M., Adriano E.A. 2014. Henneguya cuniculator sp. nov., a parasite of spotted surubim Pseudoplatystoma corruscans in the Sao Francisco Basin, Brazil. Diseases of Aquatic Organisms, 107, 211-221. DOI: 10.3354/dao02685 10.3354/dao02685Search in Google Scholar PubMed

Posada D. 2008. jModelTest: phylogenetic model averaging. Molecular Biology and Evolution, 25, 1253-1256. DOI: 10.1093/ molbev/msn08310.1093/molbev/msn083Search in Google Scholar PubMed

Pote L.M., Hanson L.A., Shivaji R. 2000. Small subunit ribosomal RNA sequences link the cause of proliferative gill disease in channel catfish to Henneguya n. sp. (Myxozoa: Myxosporea). Journal of Aquatic Animal Health, 12, 230-240. DOI:10.1577/1548-667(2000)012<0230:SSRRSL>2.0.CO;2Search in Google Scholar

Rambaut A. FigTree v1.1.1: Tree figure drawing tool, 2008. Disponivel em:<>. Access: 19 June 2014Search in Google Scholar

Soares C.M., Hayashi C., Furuya V.R.B., Furuya W.M., Galdioli E.M. 2000. Substituicao parcial e total da proteina do farelo de soja pela do farelo de canola na alimentacao de alevinos de piavucu (Leporinus macrocephalus, L.). Revista Brasileira de Zootecnia, 29, p.15-22. DOI: 2000000100003Search in Google Scholar

Thompson J.D., Gibson T.J., Plewniak F., Jeanmoungin F., Higgins D.G. 1997. The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876-488210.1093/nar/25.24.4876Search in Google Scholar PubMed PubMed Central

Received: 2014-10-23
Revised: 2015-2-2
Accepted: 2015-3-16
Published Online: 2015-6-16
Published in Print: 2015-9-1

© W. Stefański Institute of Parasitology, PAS

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