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Licensed Unlicensed Requires Authentication Published by De Gruyter June 16, 2015

Wild boars meat as a potential source of human trichinellosis in Poland: current data

Bożena Moskwa, Aleksandra Cybulska, Aleksandra Kornacka, Władysław Cabaj and Justyna Bień
From the journal Acta Parasitologica

Abstract

Trichinellosis is an epidemiological problem with a global distribution. In Poland a substantial increase of the wild boar population has been observed since 2010, together with an increased incidence of trichinellosis after ingestion of raw or undercooked wild boar products containing Trichinella spp. larvae. However, the actual number of human cases remains particularly difficult to determine. The aim of the present study was to determine the current prevalence and spread of these parasites within wild boars. The diaphragm pillars and tongue from 833 wild boars were collected from 2010 to 2014, as well as one wild boar meat sausage known to be a source of infection. The samples were tested for Trichinella spp. using pepsin digestion. Recovered larvae were identified at species level by multiplex polymerase chain reaction (multiplex PCR). The overall prevalence in all examined samples was found to be 2.0% (17/833). Recovered larvae were identified as T. spiralis and T. britovi (9/18 and 5/18, respectively). T. spiralis larvae were isolated from the sausage. Mixed infection was confirmed only once. Three isolates were not identified. The results of our study confirm that the wild boar plays a key role in the maintenance of Trichinella nematodes through the sylvatic cycle.

References

Akkoc N., Kuruuzum Z., Akar S., Yuce A., Onen F., Yapar N., Ozgenc O., Turk M., Ozdemir D., Avci M., Guruz Y., Oral A.M., Pozio E. 2009. Izmir Trichinellosis Outbreak Study Group. A large-scale outbreak of trichinellosis caused by Trichinella britovi in Turkey. Zoonoses Public Health, 56, 65-70. DOI: 10.1111/j.1863-2378.2008.01158.x 10.1111/j.1863-2378.2008.01158.xSearch in Google Scholar

Antolová D., Reiterová K., Dubinský P. 2006. The role of wild boars (Sus scrofa) in circulation of trichinellosis, toxocarosis and ascariosis in the Slovak Republic. Helminthology, 43, 92-7. DOI: 10.2478/s11687-006-0018-0Search in Google Scholar

Bilska-Zając E., Różycki M., Chmurzyńska E., Cencek T., Kusyk P., Próchnik M., Bocian L. 2013a. Trichinella species observed in the wild boar population in Poland. In: Proceedings of the International Conference "The current situation of trichinosis in Poland", 7-11 November 2013; Puławy, 40-41[in Polish] Search in Google Scholar

Bilska-Zając E., Różycki M., Chmurzyńska E., Marucci G., Cencek T., Karamon J., Bocian L. 2013b. Trichinella species circulating in wild boar (Sus scrofa) populations in Poland. International Journal for Parasitology: Parasites and Wildlife, 2, 211-213. DOI: 10.1016/j.ijppaw.2013.05.00410.1016/j.ijppaw.2013.05.004Search in Google Scholar

Cabaj W. 2006. Wild and domestic animals as permanent Trichinella reservoir in Poland. Wiadomości Parazytologiczne 52, 175-179 [in Polish] Search in Google Scholar

Cabaj W., Moskwa B., Pastusiak K., Bień J. 2005. Trichinellosis in wildlife animals a constant risk to human health in Poland. Kosmos, 54, 95-103 [in Polish] Search in Google Scholar

Cabaj W., Moskwa B., Pastusiak K., Malczewski A. 2004. Trichinellosis in wildlife animals and pigs in Poland. Medycyna Weteryanryjna, 60, 80-3 [in Polish with English summary] Search in Google Scholar

Cabaj W., Pozio E., Moskwa B., Malczewski A. 2000. Trichinella britovi and T. spiralis in red foxes (Vulpes vulpes) in Poland. Acta Parasitologica, 45, 340-344Search in Google Scholar

Cybulska A., Bień J., Kornacka K., Cabaj W., Moskwa B. 2014. Wild boars as a reservoir of nematodes from genus Trichinella in the sylvatic cycle in Poland. In: Book of Abstracts of V4 Parasitological Meeting, Slovakia, 25-30 May 2014, Stara Lešna, Slovakia, 70-71Search in Google Scholar

Chmurzyńska E., Różycki M., Bilska-Zając E., Nockler K., Mayer- Scholl A., Pozio E., Cencek T., Karamon J. 2013. Trichinella nativa in red foxes (Vulpes vulpes) of Germany and Poland: possible different origins. Veternary Parasitology, 198, 254-257. DOI: 10.1016/j.vetpar.2013.07.03410.1016/j.vetpar.2013.07.034Search in Google Scholar

De Bruyne A., Ancelle T., Vallee I., Boireau P., Dupouy-Camet J. 2006. Human trichinellosis acquired from wild boar meat: a continuing parasitic risk in France. Eurosurveillance, 11, E060914.5.10.2807/esw.11.37.03048-enSearch in Google Scholar

Dupouy-Camet J. 2000. Trichinellosis: a worldwide zoonosis. Veterinary Parasitology, 93, 191-20010.1016/S0304-4017(00)00341-1Search in Google Scholar

European Commission, 2005. Regulation (EC) no. 2075/2005 of the European Parliament and of the Council of 5 December 2005 laying down specific rules on official controls for Trichinella in meat. Official Journal of the European Union L, 338, 60-82Search in Google Scholar

Feidas H., Kouam M.K., Kantzoura V., Theodoropoulos G. 2014. Global geographic distribution of Trichinella species and genotypes. Infection, Genetics and Evolution, 26, 255-266. DOI: 10.1016/j.meegid.2014.06.00910.1016/j.meegid.2014.06.009Search in Google Scholar

Gottstein B., Pozio E., No_ckler K. 2009. Epidemiology, Diagnosis, Treatment, and Control of Trichinellosis. Clinical Microbiology Reviews, 22,127-45. DOI: 10.1128/CMR.00026-0810.1128/CMR.00026-08Search in Google Scholar

Hurníková Z., Dubinský P. 2009. Long-term survey on Trichinella prevalence in wildlife of Slovakia. Veterinary Parasitology, 159, 276-280. DOI: 10.1016/j.vetpar.2008.10.05610.1016/j.vetpar.2008.10.056Search in Google Scholar

Jokelainen P., Näreaho A., Hälli O., Heinonen M., Sukura A. 2012. Farmed wild boars exposed to Toxoplasma gondii and Trichinella spp. Veterinary Parasitology, 187, 323-327. DOI: 10.1016/j.vetpar.2011.12.02610.1016/j.vetpar.2011.12.026Search in Google Scholar

Kocięcki J., Czaplicka E., Kocięcka W. 2014. Ocular system involvment in the course of human trichinellosis. Pathological and diagnostic aspects. Acta Parasitologica, 59, 493-500. DOI: 10.2478/s11686-014-0272-910.2478/s11686-014-0272-9Search in Google Scholar

Kozdrowski R., Dubiel A. 2004. Wild boar reproductive biology. Medycyna Weterynaryjna, 60, 1251-1253 [in Polish] Search in Google Scholar

Mayer-Scholl A., Broglia A., Reckinger S., Nöckler K. 2014. Polymerase chain reaction - restriction fragment length polymorphism analysis for the differentiation of Trichinella nativa and Trichinella britovi. Veterinary Parasitology, 203, 247-249. DOI: 10.1016/j.vetpar.2014.01.01510.1016/j.vetpar.2014.01.015Search in Google Scholar

Meng X.L., Lindsay D.S., Sriranganathan N. 2012. Wild boars as sources for infectious diseases in livestock and humans. Philosophical Transactions of the Royal Society B, 364, 2697-2707. DOI: 10.1098/rsbt.2009.0086Search in Google Scholar

Moskwa B., Goździk K., Bień J., Borecka A., Gawor J., Cabaj W. 2013a. First report of Trichinella pseudospiralis in red foxes (Vulpes vulpes) in Poland. Acta Parasitologica, 58, 149-154. DOI: 10.2478/s11686-013-0121-210.2478/s11686-013-0121-2Search in Google Scholar

Moskwa B., Bień J., Kornacka A., Cybulska A., Cencek T., Kalisińska E., Pilarczyk B., Cabaj W. 2013b. The presence of Trichinella spp. in the sylvatic environment - current data. In: Proceedings of the International Conference "Current situation of trichinosis in Central Europe": 7-11 November 2013; Puławy, Poland, 5-7 [in Polish] Search in Google Scholar

Murrell K.D., Pozio E. 2011. Worldwide Occurrence and Impact of Human Trichinellosis, 1986-2009. Emerging Infectious Diseases, 17, 2194-220210.3201/eid1712.110896Search in Google Scholar

Nowosad P., Pozio E. 1998. First report of Trichinella britovi in wildlife from Poland. Acta Parasitologica, 43, 236-237Search in Google Scholar

Pannwitz G., Mayer-Scholl A., Balicka-Ramisz A., Nőckler K. 2008. Increased prevalence of Trichinella spp. Northeastern Germany, 2008. Emerging Infectious Diseases, 16, 936-942. DOI: 10.3201/eid1606.09162910.3201/eid1606.091629Search in Google Scholar

Pozio E. 2007.World distribution of Trichinella spp. infections in animals and humans. Veterinary Parasitology, 149, 3-21. DOI: 10.1016/j.vetpar.2007.07.00210.1016/j.vetpar.2007.07.002Search in Google Scholar

Pozio E. 2013. The opportunistic nature of Trichinella - exploitation of new geographies and habitats. Vet Parasitol, 194, 128-132. DOI: 10.1016/j.vetpar.2013.01.03710.1016/j.vetpar.2013.01.037Search in Google Scholar

Pozio E., Rinaldi L., Marucci G., Musella V., Galati F., Cringoli G., Boireau P., La Rosa G. 2009. Hosts and habitats of Trichinella spiralis and Trichinella britovi in Europe. International Journal for Parasitology, 39, 71-79. DOI: 10.1016/j.ijpara.2008.06.00610.1016/j.ijpara.2008.06.006Search in Google Scholar

Pozio E., Zarlenga D.S. 2013. New pieces of the Trichinella puzzle. International Journal for Parasitology, 43, 983-997. DOI: 10.1016/j.ijpara.2013.05.01010.1016/j.ijpara.2013.05.010Search in Google Scholar

Ramisz A., Grupiński T., Balicka-Ramisz A., Udała J., Luarans L. 2011. Prevalence of Trichinella spp. in red foxes and wild boars in the Western Pomerania region. Bulletin of the Veterinary Institute in Pulawy, 55, 199-201Search in Google Scholar

Regulation of the Minister of Agriculture and Rural Development dated October 21, 2010, Journal of Laws, No. 207, item 1370Search in Google Scholar

Sadkowska-Todys M., Gołąb E. 2009. Trichinellosis in Poland in 2007. Przegląd Epidemiologiczny, 63, 263-266 [in Polish with English summary] Search in Google Scholar

Sadkowska-Todys M., Gołąb E. 2013. Trichinellosis in Poland in 2011. Epidemiological Review, 67, 259-261Search in Google Scholar

Serrano F.J., Perez-Martin E., Reina D., Nieto C.G., Navarrete I., Murrell K.D. 1998. Intensity of natural Trichinella spiralis and T. britovi infections in animal hosts of Extremadura (Spain) and its repercussion for diagnosis by direct methods. Research and Reviews in Parasitology, 58, 117-20Search in Google Scholar

Wu Z., Nagano I., Pozio E., Takahashi Y.1999. Polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) for the identification of Trichinella isolates. Parasitology, 118, 211-21810.1017/S0031182098003679Search in Google Scholar

Zarlenga D.S., Chute M.B., Martin A., Kapel C.M.O. 1999. A multiplex PCR for unequivocal differentiation of all encapsulated and non-encapsulated genotypes of Trichinella. International Journal for Parasitology, 29, 1859-1867 10.1016/S0020-7519(99)00107-1Search in Google Scholar

Received: 2014-11-10
Revised: 2015-4-21
Accepted: 2015-4-23
Published Online: 2015-6-16
Published in Print: 2015-9-1

© W. Stefański Institute of Parasitology, PAS