Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter June 16, 2015

Molecular characterization of Fascioloides magna (Trematoda: Fasciolidae) from south-western Poland based on mitochondrial markers

Ivica Králová-Hromadová, Eva Bazsalovicsová and Aleksander W. Demiaszkiewicz
From the journal Acta Parasitologica

Abstract

The giant liver fluke, Fascioloides magna, is a veterinary important liver parasite of free living and domestic ruminants. This originally North American parasite was introduced along with its cervid hosts to Europe where it has established three permanent natural foci - in northern Italy, central and southern parts of the Czech Republic and the Danube floodplain forests. The first record on fascioloidosis in Poland originated from the Lower Silesian Forest in south-western Poland and since then an occurrence of F. magna in this country has not been documented. Recently, the parasitological examination of red deer (Cervus elaphus elaphus) from the Lower Silesian Wilderness (south-western Poland) revealed the presence of F. magna eggs. In order to determine the genetic interrelationships of the Polish giant liver fluke individuals, they were molecularly analyzed by mitochondrial cytochrome c oxidase subunit I (cox1) and nicotinamide dehydrogenase subunit I (nad1) and compared with haplotypes of so far studied European populations of the parasite. The study revealed the genetic uniformity of F. magna specimens from Poland with part of individuals from the Czech natural focus.

Note: Nucleotide sequence data reported in this paper are available in the GenBankTM, EMBL and DDBJ databases under the accession numbers KP635008-9.

References

Almarza N. 1935. The liver fluke in sheep (in German). Description of new species. Infectious diseases and Hygiene of Pets, 47, 195-202Search in Google Scholar

Apostolo C. 1996. The naturalistic aspects: flora, fauna and the environment. In: (Eds. M. Lupo, M. Paglieri, C. Apostolo, E. Vaccarino and M. Debernardi) La Mandria Storia e natura del Parco. Edizioni Eda, Torino, 67−98Search in Google Scholar

Bazsalovicsová E., Králová-Hromadová I., Špakulová M., Reblánová M., Oberhauserová K. 2010. Determination of ribosomal internal transcribed spacer 2 (ITS2) interspecific markers in Fasciola hepatica, Fascioloides magna, Dicrocoelium dendriticum and Paramphistomum cervi (Trematoda), parasites of wild and domestic ruminants. Helminthologia, 47, 76-82. DOI: 10.2478/s11687-010-0011-110.2478/s11687-010-0011-1Search in Google Scholar

Bazsalovicsová E., Králová-Hromadová I., Radvánszky J., Beck R. 2013. The origin of the giant liver fluke, Fascioloides magna (Trematoda: Fasciolidae) from Croatia determined by highresolution melting screening of mitochondrial cox1 haplotypes. Parasitology Research, 112, 2661-2666. DOI: 10.1007/ s00436-013-3433-010.1007/s00436-013-3433-0Search in Google Scholar

Chroustová E., Hůlka J., Jaroš J. 1980. Prevence a terapie fascioloidózy skotu bithionosulfoxidem. Veterinární medicína, Praha, 25, 557-563. (in Czech) Search in Google Scholar

Demiaszkiewicz A.W. 2014. Migrations and the introduction of wild ruminants as a source of parasite exchange and emergence of new parasitoses. Annals of Parasitology, 60, 25-30Search in Google Scholar

Erhardová-Kotrlá B. 1971. The occurrence of Fascioloides magna (Bassi, 1875) in Czechoslovakia, Publishing House of the Czechoslovak Academy of Sciences, Academia, Prague, 155 pp.Search in Google Scholar

Kašný M., Beran L., Siegelová V., Siegel T., Leontovyč R., Beránková K., Pankrác J., Kostáková M., Horák P. 2012. Geographical distribution of the giant liver fluke (Fascioloides magna) in the Czech Republic and potential risk of its further spread. Veterinární Medicína, 57, 101-10910.17221/5256-VETMEDSearch in Google Scholar

Králová-Hromadová I., Špakulová M., Horáčková E., Turčeková Ľ., Novobilský A., Beck R., Koudela B., Marinculić A., Rajský D., Pybus M. 2008. Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica. Journal of Parasitology, 94, 58-67. DOI: 10.1645/GE-1324.110.1645/GE-1324.1Search in Google Scholar

Králová-Hromadová I., Bazsalovicsová E., Štefka J., Špakulová M., Vávrová S., Szemes T., Tkach V., Pybus M. 2011. Multiple origins of European populations of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae), a liver parasite of ruminants. International Journal for Parasitology, 41, 373-383. DOI: 10.1016/j.ijpara.2010.10.01010.1016/j.ijpara.2010.10.010Search in Google Scholar

Majoros G., Sztojkov V. 1994. Appearance of the large American liver fluke Fascioloides magna (Bassi, 1875) (Trematoda: Fasciolata) in Hungary. Parasitologia Hungarica, 27, 27−38.Search in Google Scholar

Marinculić A., Džakula N., Janicky Z., Lućinger S., Živićniak T. 2002. Appearance of American liver fluke (Fascioloides magna Bassi, 1875) in Croatia - a case report. Veterinarski Archiv, 72, 319−325Search in Google Scholar

Mariković D., Kukolj V., Aleksić-Kovačević S., Jovanović M., Knežević M. 2013. The role of hepatic myofibroblasts in liver cirrhosis in fallow deer (Dama dama) naturally infected with giant liver fluke (Fascioloides magna). BMC Veterinary Research 9, 45. DOI:10.1186/1746-6148-9-4510.1186/1746-6148-9-45Search in Google Scholar

Novobilský A., Horáčková E., Hirtová L., Modrý D., Koudela B. 2007. The giant liver fluke Fascioloides magna (Bassi, 1875) in cervids in the Czech Republic and potential of its spreading to Germany. Parasitology Research, 100, 549-553. DOI: 10.1007/s00436-006-0299-410.1007/s00436-006-0299-4Search in Google Scholar

Pybus M.J. 2001. Liver flukes. In: (Eds. W.M. Samuel, M.J. Pybus and A.A. Kocan) Parasitic diseases of wild mammals, 2nd edition. Iowa State Press, Iowa City, 121-14910.1002/9780470377000.ch6Search in Google Scholar

Pyziel A.M., Demiaszkiewicz A.W., Kuligowska I. 2014. Molecular identification of Fascioloides magna (Bassi, 1875) from red deer from South-Western Poland (Lower Silesian wilderness) on the basis of internal transcribed spacer 2 (ITS-2). Polish Journal of Veterinary Sciences, 17, 523-525. DOI: 10.2478/ pjvs-2014-007110.2478/pjvs-2014-0077Search in Google Scholar

Rajský D., Patus A., Bukovjan K. 1994. Prvý nález Fascioloides magna (Bassi, 1875) na Slovensku. Slovenský Veterinársky Časopis, 19, 29−30. (in Slovak) Search in Google Scholar

Salomon S. 1932. Fascioloides magna bei deutschem Rotwild. Berliner Tierärztliche Wochenschrift, 48, 627-628. (in German) Search in Google Scholar

Skog A., Zachos F.E., Rueness E.K., Feulner P.G.D., Mysterud A., Langvatn R., Lorenzini R., Hmwe S.S., Lehoczky I., Hartl G.B., Stenseth N.C., Jakobsen K.S. 2009. Phylogeography of red deer (Cervus elaphus) in Europe. Journal of Biogeography, 36, 66-77. DOI: 10.1111/j.1365-2699.2008.01986.x 10.1111/j.1365-2699.2008.01986.xSearch in Google Scholar

Slusarski W. 1955. Studia nad europejskimi przedstawicielami przywry Fasciola magna (Bassi, 1875) Stiles, 1894. Acta Parasitologica Polonica, 3, 1-59. (in Polish) Search in Google Scholar

Ullrich K. 1930. Über das Vorkommen von seltenen oder wenig bekannten Parasiten der Säugetiere und Vögel in Böhmen und Mähren. Prager Archiv Tiermedicine, 10 A (1/2), 19−43. (in German) Search in Google Scholar

Werle E., Schneider C., Renner M., Volker M., Fiehn W. 1994. Convenient singlestep, one tube purification of PCR products for direct sequencing. Nucleic Acids Research, 22, 4354-4355 10.1093/nar/22.20.4354Search in Google Scholar

Winkelmayer R., Prosl H. 2001. Riesenleberegel − jetzt auch bei uns? Österreichisches Weidwerk, 3, 42−44. (in German)Search in Google Scholar

Received: 2015-1-16
Revised: 2015-2-23
Accepted: 2015-4-18
Published Online: 2015-6-16
Published in Print: 2015-9-1

© W. Stefański Institute of Parasitology, PAS