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Licensed Unlicensed Requires Authentication Published by De Gruyter June 16, 2015

Molecular characterization of muscle-parasitizing didymozoid from a chub mackerel, Scomber japonicus

Niichiro Abe and Mitsuru Okamoto
From the journal Acta Parasitologica

Abstract

Didymozoids found in the muscles of marine fish are almost always damaged because they are usually found after being sliced. Therefore, identifying muscle-parasitizing didymozoids is difficult because of the difficulty in collecting non-damaged worms and observing their organs as key points for morphological identification. Moreover, muscle-parasitizing didymozoids are not easily found because they parasitize at the trunk muscles. Therefore, muscle-parasitizing didymozoid classification has not progressed because there are few opportunities to detect them. Our recent report was the first to describe the usefulness of sequencing analysis for discrimination among muscle-parasitizing didymozoids. Recently, we found a didymozoid in the trunk muscle of a chub mackerel Scomber japonicus. The present study genetically compares the present isolate with other muscle-parasitizing didymozoids. The present isolate differs markedly from the previously unidentified didymozoid from an Atlantic mackerel S. scombrus by phylogenetic analysis of 18S rDNA. It also differs from other muscle-parasitizing didymozoids from other host species based on phylogenetic analyses of 18S, 28S rDNAs, and coxI loci. These results suggest that sequencing analysis is useful for the discrimination of muscle-parasitizing didymozoids. Combining the present data with earlier data for sequencing analysis, muscle-parasitizing didymozoids from seven marine fish species were classified as seven species. We proposed appellations for six distinct muscle-parasitizing didymozoids for future analysis: sweetlips fish type from Diagramma pictum and Plectorhinchus cinctus, red sea bream type from Pagrus major, flying fish type from Cypselurus heterurus, Atlantic mackerel type from Scomber scombrus, chub mackerel type from S. japonicus, and purple rockcod type from Epinephelus cyanopodus.

References

Abe N., Okamoto M., Maehara T. 2014. Molecular characterization of muscle-parasitizing didymozoids in marine fishes. Acta Parasitologica, 59, 354-358. DOI: 10.2478/s11686-014-0234-210.2478/s11686-014-0234-2Search in Google Scholar

Cribb T.H., Williams A. 1992. Gonapodasmius williamsoni sp. n. (Digenea: Didymozoidae) from the pink snapper, Pagrus auratus (Teleostei: Sparidae) in western Australia. Journal of Helminthological Society of Washington, 59, 153-158Search in Google Scholar

Ishii N. 1935. Studies of the family Didymozooidae (Monticelli, 1888). Japanese Journal of Zoology, 6, 279-335Search in Google Scholar

Mladineo I., Bott N.J., Nowak B.F., Block B.A. 2010. Multilocus phylogenetic analyses reveal that habitat selection drives the speciation of Didymozoidae (Digenea) parasitizing Pacific and Atlantic bluefin tunas. Parasitology, 137, 1013-1025. DOI: 10.1017/S003118200999170310.1017/S0031182009991703Search in Google Scholar

Momoyama K., Tensha K. 2006. Ugly-looking parasitic infections and other abnormalities of wild fish and shellfish caught in the coastal or inland waters around or in Yamaguchi Prefecture. Bulletin of Yamaguchi Prefectural Fishery Research Center, 4, 143-161Search in Google Scholar

Nakajima K., Sugiyama T., Egusa S. 1974. A trematodes, Gonapodasmius okushimai ISHII, found in the muscle of red sea bream, Pagrosomus major. Fish Pathology, 8, 175-17610.3147/jsfp.8.175Search in Google Scholar

Okamoto M. 2011. The parasites of fishes and shellfishes caught in the southwestern Sea of Japan off Shimane Prefecture. Report of Shimane Prefectural Fishery Technology Center, 3, 55-68Search in Google Scholar

Okushima A. 1921. A new aschelminth parasitic in muscle of Pagrosomus major. Fukuoka Ika Daigaku Zasshi, 14, 463-470Search in Google Scholar

Olson P.D., Cribb T.H., Tkach V.V., Bray R.A., Littlewood D.T.J. 2003. Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology, 33, 733-755. DOI: 10.1016/S0020-7519(03)00049-310.1016/S0020-7519(03)00049-3Search in Google Scholar

Pascual S., Abollo E., Azevedo C. 2006. Host-parasite interaction of a muscle-infecting didymozoid in the Atlantic mackerel Scomber scombrus L. ICES Journal of Marine Science, 63, 169-175. DOI: 10.1016/j.icesjms.2005.08.01010.1016/j.icesjms.2005.08.010Search in Google Scholar

Pozdnyakov S.E., Gibson D.I. 2008. Family Didymozooidae Monticelli, 1888. In Keys to the Trematoda Vol. 3, Bray R.A., Gibson D.I., Jones A. (Eds.). CAB International and Natural Museum, London, U.K., pp. 84810.1079/9780851995885.0631Search in Google Scholar

Yagi K., Terashima H., Takano K., Uraguchi K. 2007. Molecular identification of animal foreign substances in foods and food products: Identification of a parasite in the muscle of Crescent sweetlips Plectorhinchus cinctus. Report of Hokkaido Institute of Public Health, 57, 97-99Search in Google Scholar

Received: 2014-10-10
Revised: 2015-3-23
Accepted: 2015-3-26
Published Online: 2015-6-16
Published in Print: 2015-9-1

© W. Stefański Institute of Parasitology, PAS