Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter September 25, 2015

Tritrichomonas foetus infection in cat – first detection in Poland

Joanna Dąbrowska, Jacek Karamon, Maciej Kochanowski, Roman Jędryczko and Tomasz Cencek
From the journal Acta Parasitologica

Abstract

Tritrichomonas foetus, a parasite of cattle reproductive system, has been recently discovered as a cause of disease in cats in many countries. T. foetus infects and colonizes cat’s ileum, caecum, colon and can lead to enteritis. This paper presents the first clinical case of cat intestinal trichomonosis caused by T. foetus in Poland. The material for this study was a smear collected from a 6-month-old male British Shorthair cat. The presence of parasitic protozoan was determined via microscopic examination and confirmed by amplification of T. foetus rDNA using polymerase chain reaction (PCR) technique. In the first PCR reaction, a DNA of Trichomonadidae was identified and in the second PCR, T. foetus was detected. The T. foetus positive products from the second PCR reaction were sequenced. Interpretation of the sequencing results of obtained amplicons by comparing them with the GenBank database proved that the causative agent, in this case, was T. foetus.

References

Bisset S.A., Gowan R.A., O’Brien C.R., Stone M.R., Gookin J.L. 2008. Feline diarrhoea associated with Tritrichomonas cf. foetus and Giardia co-infection in an Australian cattery. Australian Veterinary Journal, 86, 440-443. DOI:10.1111/j.1751-0813.2008.00356.x 10.1111/j.1751-0813.2008.00356.xSearch in Google Scholar

BonDurant R.H., Campero C.M., Anderson M.L., Hoosear K.A.. 2003. Detection of Tritrichomonas foetus by polymerase chain reaction in culture isolates, cervicovaginal mucus, and formalin-fixed tissues from infected heifers and fetuses. Journal Veterinary Diagnostic Investigation, 15, 579-58410.1177/104063870301500613Search in Google Scholar

Carvalho K.P., Gadelha A.P. 2007. Effects of three benzimidazoles on growth, general morphology and ultrastructure of Tritrichomonas foetus. FEMS Microbiology Letters, 275, 292-300. DOI: 10.1111/j.1574-6968.2007.00897.x 10.1111/j.1574-6968.2007.00897.xSearch in Google Scholar

Da Silva N.S., Ribeiro Cde M., Machado A.H., Pacheco-Soares C. 2007. Ultrastructural changes in Tritrichomonas foetus after treatments with A1PcS4 and photodynamic therapy. Veterinary Parasitology, 146, 175-181. DOI:10.1016/j.vetpar.2007.02.00610.1016/j.vetpar.2007.02.006Search in Google Scholar

Felleisen R.S.J., Lambelet N., Bachmann P., Nicolet J., Muller N., Gottstein B. 1998. Detection of Tritrichomonas foetus by PCR and DNA Enyzme Imunnoassay Based on rRNA Gene Unit Sequences. Journal Clinical Microbiology, 36, 513-51910.1128/JCM.36.2.513-519.1998Search in Google Scholar

Foster D.M., Gookin J.L., Poore M.F., Stebbins M.E., Levy M.G. 2004 Outcome of cats with diarrhoea and Tritrichomonas foetus infection. Journal American Veterinary Medical Association, 225, 888-89210.2460/javma.2004.225.888Search in Google Scholar

Frey C.F., Schild M., Hemphill A., Stunzi P., Muller N., Gottstein B., Burgener I.A.. 2009. Intestinal Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR. Parasitology Research, 104, 783-788. DOI: 10.3347/kjp.2010.48.3.24710.3347/kjp.2010.48.3.247Search in Google Scholar

Gookin J.L., Breitschwerdt E.B., Levy M.G., Gager R.B., Benrud J.G. 1999. Diarrhea associated with trichomonosis in cats. Journal American Veterinary Medical Association, 215, 1450-4Search in Google Scholar

Granger B.L., Warwood S.J., Benchimol M., De Souza W. 2000. Transient invagination of flagella by Tritrichomonas foetus. Parasitology Research, 86, 699-709. DOI: 10.1007/PL 00008555Search in Google Scholar

Gray S.G., Hunter S.A., Stone M.R., Gookin J.L. 2010. Assessement of reproductive tract disease in cats at risk for Tritrichomonas foetus infection. AVMA - American Journal of Veterinary Research, 71, 76-81. DOI: 10.2460/ajvr.71.1.7610.2460/ajvr.71.1.76Search in Google Scholar

Gunn-Moore D.A., McCann T.M., Reed N., Simpson K.E., Tennant B. 2007. Prevalence of Tritrichomonas foetus infection in cats with diarrhoea in the UK. Journal Feline Medicine Surgery, 9, 214-21810.1016/j.jfms.2007.01.003Search in Google Scholar

Hammond D.M., Bartlett D.E. 1943. The distribution of Trichomonas foetus in the preputial cavity of infected bulls. American Journal Veterinary Research, 4, 143-149Search in Google Scholar

Holliday M., Deni D., Gunn-Moore D.A.. 2009. Tritrichomonas foetus infection in cats with diarrhoea in a rescue colony in Italy. Journal of Feline Medicine and Surgery, 11, 131-134. DOI: 10.1016/j.jfms.2008.06.00410.1016/j.jfms.2008.06.004Search in Google Scholar

Honigberg B.M. 1978. Parasitic protozoa. II. Intestinal flagellates, histomonads, trichomonads, amoeba, opalinids and ciliates. Academic Press, Inc New York, N.Y.Search in Google Scholar

Klein B., Langbein-Detsch I., Muller E., Heusinger A. 2010. Pravalenz von Tritrichomonas foetus in Kotproben von Katzen mit Durchfall aus Deutschland. Kleintiermedizin, 48, 247-251Search in Google Scholar

Kuehner K.A., Marks S.L., Kass P.H., Sauter L.C., Grahn R.A., Barutzki D., Hartman K. 2011. Tritrichomonas foetus infection in purebred cats in Germany: prevalence of clinical signs and the role of co-infection with other enteroparaistes. Journal Feline Medicine Surgery, 13, 251-258. DOI: 10.1016/ j.jfms.2010.12.00210.1016/j.jfms.2010.12.002Search in Google Scholar

Lim S., Park S.I., Ahn K.S., Oh D.S., Ryu J.S., Shin S.S. 2010. First Report of Feline Intestinal Trichomoniasis Caused by Tritrichomonas foetus in Korea. Korean Journal Parasitology, 48, 247-251. DOI: 10.3347/kjp.2010.48.3.24710.3347/kjp.2010.48.3.247Search in Google Scholar

Profizi C., Cian A., Meloni D., Hugonnard M., Lambert V., Groud K., Gagon A.C., Viscogliosi E., Zenner L. 2013. Prevalence of Tritrichomonas foetus infections in French catteries. Veterinary Parasitology, 196, 50-55. DOI: 10.1016/j.vetpar.2013.01.02110.1016/j.vetpar.2013.01.021Search in Google Scholar

Stauffer S.H., Birkenheuer A.J., Levy M.G., Marr H., Gookin J.L. 2008. Evaluation of four DNA extraction methods for the detection of Tritrichomonas foetus in feline stool specimens by polymerase chain reaction. Journal of Veterinary Diagnostic Investigation, 20, 639-641. DOI:10.1177/10406387080200051810.1177/104063870802000518Search in Google Scholar

Stockdale H.D., Dillon A.R., Newton J.C., Bird R.C, Bondurant R.H., Deinnocentes P., Barney S., Bulter J., Land T., Spencer J.A., Linday D.S., Blagburn B.L. 2008. Experimental infection of cats (Felis catus) with Tritrichomonas foetus isolated from cattle. Veterinary Parasitology, 154, 156-161. DOI: 10.1016/j.vetpar.2008.02.02410.1016/j.vetpar.2008.02.024Search in Google Scholar

Stockdale H.D., Givens M.D., Dykstra C., Blagburn L. 2009. Tritrichomonas foetus infections in surveyed pet cats. Veterinary Parasitology, 160: 13-17. DOI: 10.1016/j.vetpar.2008.10.09110.1016/j.vetpar.2008.10.091Search in Google Scholar

Tysnes K., Gjerde B., Nodtvedt A., Skancke E. 2011. A cross-sectional study of Tritrichomonas foetus infection among healthy cats at shows in Norway. Acta Veterinaria Scandinavica, 53(39), 1-6. DOI: 10.1186/1751-0147-53-3910.1186/1751-0147-53-39Search in Google Scholar

Walden H.S, Dykstra Ch., Dillon A., Rodning S., Givens D., Bird R., Lindsay D. 2013. A new species of Tritrichomonas (Sarcomastigophora: Trichomonida) from the domestic cat (Felis catus). Parasitology Research, 112, 2227-2235. DOI: 10.1007/s00436-013-3381-810.1007/s00436-013-3381-8Search in Google Scholar

Walker R.L., Hayes D.C., Sawyer S.J., Nordhausen R.W., Van Hoosear K.A., BonDurant R.H. 2003. Comparison of the 5,8S rRNA gene and internal transcribed spacer regions of trichomonadid protozoa recovered from the bovine preputial cavity. Journal Veterinary Diagnostic Investigation, 15, 14-2010.1177/104063870301500104Search in Google Scholar

Xenoulis P.G. 2011. Tritrichomonas foetus: a new cause of diarrhea in cats in Greece. Journal of Hell Veterinary Medicine Society, 62(2), 132-140. DOI: 10.1016/j.jfms.2010.05.01010.1016/j.jfms.2010.05.010Search in Google Scholar

Yaeger M.J., Gookin J.L. 2005. Histologic Features associated with Tritrichomonas foetus- induced Colitis in Domestic Cats. Veterinary Pathology, 42, 797-804 10.1354/vp.42-6-797Search in Google Scholar

Received: 2014-8-25
Revised: 2015-3-2
Accepted: 2015-4-24
Published Online: 2015-9-25
Published in Print: 2015-12-1

W. Stefański Institute of Parasitology, PAS