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Licensed Unlicensed Requires Authentication Published by De Gruyter September 25, 2015

Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo

Roger D. Wumba, Josué Zanga, Kennedy M. Mbanzulu, Madone N. Mandina, Aimé K. Kahindo, Michel N. Aloni and Mathilde B. Ekila
From the journal Acta Parasitologica

Abstract

Cryptosporidium is an important protozoan parasite affecting HIV/AIDS patients. To determine the prevalence and the species of Cryptosporidium spp by developing a rapid and efficient real-time PCR-RFLP test. A cross-sectional study was conducted among HIV-infected adults from Kinshasa, the Democratic Republic of Congo. Stool specimens were examined by microscopic evaluation and real-time PCR-RFLP. Out of 242 HIV-infected adults, 10 (4.1%) cases of Cryptosporidium were identified by microscopic examination. Using PCR-RFLP, the prevalence of Cryptosporidium spp was 5.4% (n = 13). All the 13 cases of Cryptosporidium spp had the stage of AIDS of HIV infection. Extracted DNA was amplified by nested PCR targeting a 1030-bp fragment of the 18s RNA gene. RFLP analysis identified one C. parvum, four C. hominis and one non determined Cryptosporidium. The capacity to detect C. parvum, C. hominis and non-determined Cryptosporidium was present among our HIV-infected patients.

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Received: 2014-12-11
Revised: 2015-4-5
Accepted: 2015-5-8
Published Online: 2015-9-25
Published in Print: 2015-12-1

W. Stefański Institute of Parasitology, PAS