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Licensed Unlicensed Requires Authentication Published by De Gruyter September 25, 2015

Molecular studies on larvae of Pseudoterranova parasite of Trichiurus lepturus Linnaeus, 1758 and Pomatomus saltatrix (Linnaeus, 1766) off Brazilian waters

Juliana N. Borges, Luiz F. G. Cunha, Daniele F. Miranda, Cassiano Monteiro-Neto and Cláudia P. Santos
From the journal Acta Parasitologica

Abstract

Pseudoterranova larvae parasitizing cutlassfish Trichiurus lepturus and bluefish Pomatomus saltatrix from Southwest Atlantic coast of Brazil were studied in this work by morphological, ultrastructural and molecular approaches. The genetic analysis were performed for the ITS2 intergenic region specific for Pseudoterranova decipiens, the partial 28S (LSU) of ribosomal DNA and the mtDNA cox-1 region. We obtained results for the 28S region and mtDNA cox-1 that was amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships between sequences of this study and sequences from the GenBank. The morphological profile indicated that all the nine specimens collected from both fish were L3 larvae of Pseudoterranova sp. The genetic profile confirmed the generic level but due to the absence of similar sequences for adult parasites on GenBank for the regions amplifyied, it was not possible to identify them to the species level. The sequences obtained presented 89% of similarity with Pseudoterranova decipiens (28S sequences) and Contracaecum osculatum B (mtDNA cox-1). The low similarity allied to the fact that the amplification with the specific primer for P. decipiens didn´t occur, lead us to conclude that our sequences don’t belong to P. decipiens complex.

References

Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. 1990. Basic Local Alignment Search Tool. Journal of Molecular Biology, 215, 403-10. DOI: 10.1016/S0022-2836(05)80360-210.1016/S0022-2836(05)80360-2Search in Google Scholar

Barros G.C. Amato J. F.R. 1992. Larvas de Anisakideos de Peixe-espada, Trichiurus lepturus L., da costa do estado do Rio de Janeiro, Brasil. Revista Brasileira de Biologia, 53, 241-245Search in Google Scholar

Blouin M.S. 2002. Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. International Journal for Parasitology, 32, 527-53110.1016/S0020-7519(01)00357-5Search in Google Scholar

Borges J.N., Cunha L.F.G., Santos H.L.C., Monteiro-Neto C., Santos C.P. 2012. Morphological and molecular diagnosis of Anisakid Nematode larvae from Cutlassfish (Trichiurus lepturus) off the coast of Rio de Janeiro, Brazil. PlosOne 7: e40447. DOI: 10.1371/journal.pone.004044710.1371/journal.pone.0040447Search in Google Scholar

Bowles J., Blair D., McManus D.P. 1992. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology, 54, 165-17310.1016/0166-6851(92)90109-WSearch in Google Scholar

Caldwell D.K., Caldwell M.C. 1989. Pygmy sperm whale Kogia breviceps (de Blainville, 1838); Dwarf sperm whale Kogia simus Owen, 1866. In: (Eds. S. H. Ridgway and S. R. Harrison) Handbook of Marine Mammals Vol. 4: River Dolphins and the Larger Toothed Whales. Academic Press, London, pp. 235-260Search in Google Scholar

Froese R., Pauly D. 2011. FishBase. World Wide Web electronic publication.www.fishbase.org - version (December 2011). Accessed 10 December 2011Search in Google Scholar

Felsenstein J. 1981. Evolutionary Trees from DNA Sequences: A Maximum Likelihood Approach. Journal of Molecular Biology Evolution, 17, 368-37610.1007/BF01734359Search in Google Scholar

Haimovici M., Krug L.C. 1996. Fishery and biology of the enchova Pomatomus saltatrix in southern Brazil. Australian Journal of Marine & Freshwater Research, 47, 357-363. DOI:10. 1071/MF996035710.1071/MF9960357Search in Google Scholar

Kimura M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16,111-120. DOI: 10.1007/BF0173158110.1007/BF01731581Search in Google Scholar

Luque J.L., Chaves N.D. 1999. Ecologia da comunidade de metazoarios parasitos da anchova Pomatomus saltator (Linnaeus) (Osteichthyes, Pomatomidae) do litoral do estado do Rio de Janeiro, Brasil. Revista Brasileira de Zoologia, 16,711-23. DOI: 10.1590/S0101-8175199900030001010.1590/S0101-81751999000300010Search in Google Scholar

Luque J.L., Aguiar J.C., Vieira F.M., Gibson D.I., Santos C.P. 2011. Checklist of Nematoda associated with fishes of Brazil. Zootaxa, 3082, 1-8810.11646/zootaxa.3082.1.1Search in Google Scholar

Martins A.S., Haimovici M. 1997. Distribution, abundance and biological interactions of the cutlassfish Trichiurus lepturus in the southern Brasil subtropical convergence ecosystem. Fisheries Research, 30, 217-227. DOI: 10.1016/S0165-7836 (96)00566-8Search in Google Scholar

Mattiucci S., Nascetti G. 2008. Advances and trends in molecular systematics of Anisakid Nematodes, with implications for their evolutionary, ecology and host-parasite co-evolutionary processes. In: (Eds. D. Rollinson and S. I. Hay) Advances in parasitology, vol. 66, Academic Press, London, 47-14810.1016/S0065-308X(08)00202-9Search in Google Scholar

McClelland G., Misra R.K., Martell D.J. 2000. Sapatial and temporal distributions of larval sealworm (Pseudoterranova decipiens, Nematoda: Anisakinae), in Hippoglossoides platessoides (Pleuronectidae) in eastern Canada from 1980 to 1990. ICES Journal of Marine Science, 57, 69-88. DOI: 10.1006/jmsc.1999.051810.1006/jmsc.1999.0518Search in Google Scholar

Muniz-Pereira L.C., Vieira F.M., Luque J. 2010. Checklist of helminth parasites of threatened vertebrate species from Brazil. Zootaxa, 2123, 1-4510.11646/zootaxa.2123.1.1Search in Google Scholar

Nadler S.A., D’Amelio S., Dailey M.D., Paggi L., Siu S., Sakanari J.A. 2005. Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern Pacific marine mammals. The Journal of Parasitology, 91, 1413-29. DOI: 10.1645/GE-522R.110.1645/GE-522R.1Search in Google Scholar

Paraguassú A.R., Luque J.L., Alves D.R. 2000. Aspectos quantitativos do parasitismo por larvas de anisakideos (Nematoda: Ascaridoidea: Anisakidae) no pargo, Pagrus pagrus (Linnaeus, 1758) (Osteichthyes: Sparidae) do litoral do estado do Rio de Janeiro, Brasil. Contribuições Avulsas sobre a Historia Natural do Brasil, 24, 1-810.4067/S0716-07202000000100006Search in Google Scholar

Pinto R.M., Muniz-Pereira L.C., Alves V.C., Siciliano S. 2004. First report of a helminth infection for Bryde’s whale, Balaenoptera edeni Anderson, 1878 (Cetacea, Balaenopteridae). Latin American Journal of Aquatic Mammals, 3, 167-170. DOI: 10.5597/lajam0006210.5597/lajam00062Search in Google Scholar

Rêgo A.A., Vicente J.J., Santos C.P., Wekid, R.M. 1983. Parasitas de anchovas Pomatomus saltatrix (L.) do Rio de Janeiro. Ciência e Cultura, 35, 1329-1336Search in Google Scholar

Santos C.P., Lodi L. 1998. Occurrence of Anisakis physeteris Baylis, 1923 and Pseudoterranova sp. (Nematoda) in pygmy sperm whale Kogia breviceps (De Blainvillei, 1838) (Physeteridae) in northeastern coast of Brazil. Memórias do Instituto Oswaldo Cruz, 92, 187-188. DOI: 10.1590/S0074-0276199 8000200009Search in Google Scholar

São Clemente S.C., Marques M.C., Serra-Freire N.M., Lucena F.P. 1995. Analise do parasitismo de peixe espada Trichiurus lepturus (L.) do litoral do Rio de Janeiro - Brasil. Parasitología al Día, 19, 146-149Search in Google Scholar

Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA 5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731-2739. DOI: 10.1093/molbev/msr12110.1093/molbev/msr121Search in Google Scholar

Thompson J.D., Higgins D.G., Gibson T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673-4680. DOI: 10.1093/nar/22.22.467310.1093/nar/22.22.4673Search in Google Scholar

Umehara A., Kawakami Y., Araki J., Uchida A. 2008. Multiplex PCR for the identification of Anisakis simplex sensu stricto, Anisakis pegreffii and the other anisakid nematodes. Parasitology International, 57, 49-53. DOI: 10.1016/j.parint.2007.08.00310.1016/j.parint.2007.08.003Search in Google Scholar

Zerbini A.N., Secchi E.R., Siciliano S., Simoes-Lopes P.C. 1997. A review of the occurrence and distribution of whales of the genus Balaenoptera along the brazilian coast. Reports of the International Whaling Commission, 47, 407-417Search in Google Scholar

Received: 2014-7-14
Revised: 2015-4-29
Accepted: 2015-5-14
Published Online: 2015-9-25
Published in Print: 2015-12-1

W. Stefański Institute of Parasitology, PAS