Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter September 25, 2015

Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen

Yosra H. Alam-Eldin, Heba E. Abdel Aaty and Mona A. Ahmed
From the journal Acta Parasitologica

Abstract

Few molecular studies have identified the current status of cystic echinococcosis in Egypt. The present study aimed to ascertain the genotype(s) of Echinococcus granulosus responsible for human hydatidosis in different Egyptian governorates (regions). Animal isolates were collected from 40 camels, 5 pigs and 44 sheep. 27 human isolates were included in the present study. Specific PCR was performed and followed by DNA sequencing for mitochondrial 12S ribosomal RNA gene and BLAST analysis.The sheep cysts were not hydatid cysts. G6 genotype (camel starin) predominates in human, camel and pig isolates. G7 genotype (pig strain) was detected in two human isolates and one pig isolate. G1 genotype (sheep strain) was detected in one human isolate from Yemen and in no animal isolates. This is the first record of G7 in Egypt and G1 in Yemen.

References

Aaty H.E., Abdel-Hameed D.M., Alam-Eldin Y.H., El-Shennawy S.F., Aminou H.A., Makled S.S., Darweesh S.K. 2012. Molecular genotyping of Echinococcus granulosus in animal and human isolates from Egypt. Acta Tropica, 121, 125-8. DOI: 10.1016/j.actatropica.2011.10.01410.1016/j.actatropica.2011.10.014Search in Google Scholar

Abd El Baki M.H., El Missiry A.M., Abd El Aaty H.E., Mohamad A.A., Aminou H.A. 2009. Detection of G1 genotype of human cystic echinococcosis in Egypt. Journal of Egyptian Society of Parasitology, 39, 711-21Search in Google Scholar

Aboelhadid S.M., El-Dakhly K.M., Yanai T., Fukushi H., Hassanin K.M. 2013. Molecular characterization of Echinococcus granulosus in Egyptian donkeys. Veterinary Parasitology, 193, 292-296. DOI: 10.1016/j.vetpar.2012.11.01910.1016/j.vetpar.2012.11.019Search in Google Scholar

Adwan G., Adwan, K., Bdir S., Abuseir S. 2013. Molecular characterization of Echinococcus granulosus isolated from sheep in Palestine. Experimental Parasitology, 134, 195-199. DOI: 10.1016/j.exppara.2013.03.02410.1016/j.exppara.2013.03.024Search in Google Scholar

Ahmed M.E., Eltom K.H., Musa N.O., Ali, I.A., Elamin F.M., Grobusch M.P., Aradaib I.E. 2013. First report on circulation of Echinococcus ortleppi in the one humped camel (Camelus dromedaries), Sudan. BMC Veterinary Research, 9, 127. DOI: 10.1186/1746-6148-9-12710.1186/1746-6148-9-127Search in Google Scholar

Alvarez Rojas C.A., Romig T., Lightowlers M.W. 2014. Echinococcus granulosus sensu lato genotypes infecting humans - review of current knowledge. International Journal of Parasitology, 44, 9-18. DOI: 10.1016/j.ijpara.2013.08.00810.1016/j.ijpara.2013.08.008Search in Google Scholar

Azab M.E., Bishara S.A., Helmy H., Oteifa N.M., El-Hoseiny L.M., Ramzy R.M., Ahmed M.A. 2004. Molecular characterization of Egyptian human and animal Echinococcus granulosus isolates by RAPD-PCR technique. Journal of Egyptian Society of Parasitology, 34, 83-96Search in Google Scholar

Bardonnet K., Piarroux R., Dia L., Schneegans F., Beurdeley A., Godot, V., Vuitton D.A. 2002. Combined eco-epidemiological and molecular biology approaches to assess Echinococcus granulosus transmission to humans in Mauritania: occurrence of the ‘camel’ strain and human cystic echinococcosis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 96, 383-386. DOI: 10.1016/S0035-9203(02)90369-X Cardona G.A., Carmena D. 2013.A review of the global prevalence, molecular epidemiology and economics of cystic echinococcosis in production animals. Veterinary Parasitology, 192, 10-32. DOI: 10.1016/j.vetpar.2012.09.02710.1016/j.vetpar.2012.09.027Search in Google Scholar

Dinkel A., Njoroge, E.M., Zimmermann A., Wälz M., Zeyhle E., Elmahdi I.E., Mackenstedt U., Romig T. 2004. A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. International Journal of Parasitology, 34, 645-53. DOI: 10.1016/j.ijpara.2003.12.01310.1016/j.ijpara.2003.12.013Search in Google Scholar

Farjallah S., Busi M., Mahjoub M.O., Slimane B.B., Said K., D’Amelio S. 2007.Molecular characterization of Echinococcus gran ulosus in Tunisia and Mauritania by mitochondrial rrnS gene sequencing. Parassitologia, 49, 239-246Search in Google Scholar

Jabbar A., Narankhajid M., Nolan M.J., Jex A.R., Campbell B.E., Gasser R.B. 2011. A first insight into the genotypes of Echinococcus granulosus from humans in Mongolia. Molecular and Cellular Probes. 25, 49-54. DOI: 10.1016/j.mcp.2010.11.00110.1016/j.mcp.2010.11.001Search in Google Scholar

Maillard S., Benchikh-Elfegoun M.C., Knapp J., Bart J.M., Koskei P., Gottstein B., Piarroux R. 2007. Taxonomic position and geographical distribution of the common sheep G1 and camel G6 strains of Echinococcus granulosus in three African countries. Parasitology Research, 100, 495-503. DOI: 10.1007/s00436-006-0286-910.1007/s00436-006-0286-9Search in Google Scholar

McManus D.P. 2002. The molecular epidemiology of Echinococcus granulosus and cystic hydatid disease. Transactions of the Royal Society of Tropical Medicine and Hygiene, 96, 151-157. DOI: 10.1016/S0035-9203(02)90068-410.1016/S0035-9203(02)90068-4Search in Google Scholar

McManus D.P., Thompson R.C. 2003. Molecular epidemiology of cystic echinococcosis. Parasitology, 12, 37-51. DOI: 10.1017/ S003118200300352410.1017/S0031182003003524Search in Google Scholar

Omer R.A., Dinkel A., Romig T., Mackenstedt U., Elnahas A.A., Aradaib I.E., Ahmed M.E., Elmalik K.H., Adam A. 2010. A molecular survey of cystic echinococcosis in Sudan. Veterinary Parasitology, 169, 340-346. DOI: 10.1016/j.vetpar.2010.01.00410.1016/j.vetpar.2010.01.004Search in Google Scholar

Pawlowski Z.S., Stefaniak J. 2003.Cystic echinococcosis (CE) in the Poznan region in the years 1990-2000. Przeglad Epidemiologiczny, 57, 579-586Search in Google Scholar

Sadjjadi M.S. 2006. Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitology International, 55, 197-202. DOI: 10.1016/j.parint.2005.11.030 10.1016/j.parint.2005.11.030Search in Google Scholar

Schneider R., Gollackner B., Schindl M., Tucek G., Auer H. 2010. Echinococcus canadensis G7 (pig strain): an underestimated cause of cystic echinococcosis in Austria. The American Journal of Tropical Medicine and Hygiene,82, 871-874. DOI: 10.4269/ajtmh.2010.09-063910.4269/ajtmh.2010.09-0639Search in Google Scholar

Snabel V., Altintas N., D’Amelio S., Nakao M., Romig T., Yolasigmaz A., Gunes K., Turk M., Busi M., Huttner M., Sevcova D., Ito A., Altintas N., Dubinsky P. 2009. Cystic echinococcosis in Turkey: genetic variability and first record of the pig strain (G7) in the country. Parasitology Research, 105, 145-154.DOI: 10.1007/s00436-009-1376-210.1007/s00436-009-1376-2Search in Google Scholar

Taha H.A. 2012. Genetic variations among Echinococcus granulosus isolates in Egypt using RAPD-PCR. Parasitology Research, 111, 1993-2000. DOI: 10.1007/s00436-012-3046-z 10.1007/s00436-012-3046-zSearch in Google Scholar

Tawfeek G.M., Elwakil H.S., Awad N.S., El-Hoseiny L., Thabet H.S., Sarhan R.M., Darweesh S.K., Anwar W.A. 2009. Genetic variability of antigen B among Echinococcus granulosus Egyptian isolates. Korean Journal of Parasitology, 47, 259-264. DOI: 10.3347/kjp.2009.47.3.25910.3347/kjp.2009.47.3.259Search in Google Scholar

Thompson R.C., McManus D.P. 2002. Towards a taxonomic revision of the genus Echinococcus. Trends in Parasiotology, 18, 452-457. DOI: 10.1016/S1471-4922(02)02358-910.1016/S1471-4922(02)02358-9Search in Google Scholar

Yanagida T., Mohammadzadeh T., Kamhawi S., Nakao M., Sadjjadi S.M., Hijjawi N., Abdel-Hafez S.K., Sako Y., Okamoto., Ito A. 2012. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitology International, 61, 599-603. DOI: 10.1016/j.parint.2012.05.01410.1016/j.parint.2012.05.014Search in Google Scholar

Zhang L.H., Eslami A., Hosseini S.H., McManus D.P. 1998. Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers. The American Journal of Tropical Medicine and Hygiene, 59, 171-174 10.4269/ajtmh.1998.59.171Search in Google Scholar

Received: 2015-1-1
Revised: 2015-4-15
Accepted: 2015-5-19
Published Online: 2015-9-25
Published in Print: 2015-12-1

W. Stefański Institute of Parasitology, PAS