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Licensed Unlicensed Requires Authentication Published by De Gruyter December 30, 2015

Herd-level seroprevalence of Neospora caninum infection in dairy cattle in central and northeastern Poland

Sławomir J. Kowalczyk, Michał Czopowicz, Corinna N. Weber, Elisabeth Müller, Lucjan Witkowski and Jarosław Kaba
From the journal Acta Parasitologica


A serosurvey was carried out to estimate the herd-level seroprevalence of Neospora caninum infection in cattle in central and northeastern Poland. Ninety seven dairy cattle herds from 2 provinces of Poland (Podlaskie, 47 herds and Łodzkie, 50 herds) were randomly enrolled in the study using two-stage cluster method. A simple random selection was applied within each herd to select a sample of adult cows (≥18 month-old). A total number of 734 cows were enrolled in the study. The animals were screened with a commercial competitive ELISA (Bio-X Diagnostics, Belgium). To calculate true herd-level seroprevalence test sensitivity and specificity were adjusted from an individual- to a herd-level using FreeCalc method. The true overall herd-level seroprevalence of N. caninum infection was 56.7% (95% CI: 47.5%, 65.9%). The true herd-level seroprevalence in Podlaskie was 63.3% (95% CI: 43.0%, 83.6%) and 50.5% (95% CI: 32.8%, 68.2%) in Łodzkie province and these figures did not differ significantly between the two provinces (chi2 test p = 0.238). One hundred forty three of 734 cows (19.5%) were seropositive which gave the true overall individual-level seroprevalence of 20.1% (95% CI: 17.4%, 23.2%). Percentage of seropositive cows in each herd varied from 6% to 80%. This study is the first epidemiological investigation of herd-level seroprevalence of N. caninum infection in Polish dairy cattle population. In conclusion, the result of the study confirmed previous data that N. caninum infection is widespread in the Polish cattle population and thus should be considered as a potential cause of spontaneous abortions.


Altman D.G., Machin D., Bryant T.N., Gardner M.J. (Eds.). 2000. Statistics with confidence. BMJ Books. pp. 45-57Search in Google Scholar

Bartels C.J., Arnaiz-Seco J.I., Ruiz-Santa-Quitera A., Björkman C., Frössling J., von Blumröder D., Conraths F.J., Schares G., van Maanen C., Wouda W., Ortega-Mora L.M. 2006. Supranational comparison of Neospora caninum seroprevalences in cattle in Germany, The Netherlands, Spain and Sweden. Veterinary Parasitology, 137, 17-27. DOI: 10.1016/j.vetpar. 2005.12.016Search in Google Scholar

Bártová E., Sedlak K., Budíková M. 2015. A study of Neospora caninum and Toxoplasma gondii antibody seroprevalence in healthy cattle in the Czech Republic. Annals of Agricultural and Environmental Medicine, 22, 32-3410.5604/12321966.1141365Search in Google Scholar

Bień J., Moskwa B., Bogdaszewski M., Cabaj W. 2012. Detection of specific antibodies anti-Neospora caninum in the fallow deer (Dama dama). Research in Veterinary Science, 92, 96-98. DOI: 10.1016/j.rvsc.2010.10.01410.1016/j.rvsc.2010.10.014Search in Google Scholar

Bień J., Moskwa B., Cabaj W. 2010. In vitro isolation and identification of the first Neospora caninum isolate from European bison (Bison bonasus bonasus L.). Veterinary Parasitology, 173, 200-205. DOI: 10.1016/j.vetpar.2010.06.03810.1016/j.vetpar.2010.06.038Search in Google Scholar

Cabaj W., Choromański L., Rodgers S., Moskwa B., Malczewski A. 2000. Neospora caninum infections in dairy cows with abortion in Poland. Acta Parasitologica, 45, 113-114Search in Google Scholar

Cabaj W., Moskwa B., Pastusiak K., Gill J. 2005. Antibodies to Neospora caninum in the blood of European bison (Bison bonasus bonasus L.) living in Poland. Veterinary Parasitology, 128, 163-16810.1016/j.vetpar.2004.09.033Search in Google Scholar

Cameron A.R., Baldock F.C. 1998. A new probability formula for surveys to substantiate freedom from disease. Preventive Veterinary Medicine, 34, 1-1710.1016/S0167-5877(97)00081-0Search in Google Scholar

Čobádiová A., Víchová B., Majláthová V., Reiterová K. 2013. First molecular detection of Neospora caninum in European brown bear (Ursus arctos). Veterinary Parasitology, 197, 346-349. DOI: 10.1016/j.vetpar.2013.05.00510.1016/j.vetpar.2013.05.005Search in Google Scholar PubMed

Czopowicz M., Kaba J., Szaluś-Jordanow O., Nowicki M., Witkowski L., Frymus T. 2011. Seroprevalence of Toxoplasma gondii and Neospora caninum infections in goats in Poland. Veterinary Parasitology, 178, 339-341. DOI: 10.1016/j.vetpar.2011.01.03910.1016/j.vetpar.2011.01.039Search in Google Scholar PubMed

Dubey J.P. 2003. Review of Neospora caninum and neosporosis in animals. Korean Journal of Parasitology, 41, 1-1610.3347/kjp.2003.41.1.1Search in Google Scholar PubMed PubMed Central

Goodswen S.J., Kennedy P.J., Ellis J.T. 2013. A review of the infection, genetics, and evolution of Neospora caninum: from the past to the present. Infection, Genetics and Evolution, 13, 133-150. DOI: 10.1016/j.meegid.2012.08.01210.1016/j.meegid.2012.08.012Search in Google Scholar PubMed

Goździk K., Jakubek E.B., Björkman C., Bień J., Moskwa B., Cabaj W. 2010. Seroprevalence of Neospora caninum in free living and farmed red deer (Cervus elaphus) in Poland. Polish Journal of Veterinary Sciences, 13, 117-120Search in Google Scholar

Goździk K., Grono K. 2009. Neosporosis as an abortion cause in cattle. Magazyn Weterynaryjny, 18, 230-234 Search in Google Scholar

Goździk K., Grono K. 2010. Neospora caninum - a dangerous parasite responsible for abortion in dairy cattle. Magazyn Weterynaryjny. Choroby bydła - Monografia, 1030-1033Search in Google Scholar

Goździk K., Wrzesień R., Wielgosz-Ostolska A., Bień J., Kozak- Ljunggren M., Cabaj W. 2011. Prevalence of antibodies against Neospora caninum in dogs from urban areas in Central Poland. Parasitology Research, 108, 991-996. DOI: 10.1007/s00436-010-2143-010.1007/s00436-010-2143-0Search in Google Scholar PubMed PubMed Central

Hornok S., Edelhofer R., Hajtos I. 2006. Seroprevalence of neosporosis in beef and dairy cattle breeds in Northeast Hungary. Acta Veterinaria Hungarica, 54, 485-49110.1556/AVet.54.2006.4.6Search in Google Scholar PubMed

Machačová T., Bártová E., Di Loria A., Sedlák K., Guccione J., Fulgione D., Veneziano V. 2013. Seroprevalence and risk factors of Neospora spp. in donkeys from Southern Italy. Veterinary Parasitology, 198, 201-204. DOI: 10.1016/j.vetpar.2013.08.01310.1016/j.vetpar.2013.08.013Search in Google Scholar PubMed

Moskwa B., Goździk K., Bień J., Kornacka A., Cybulska A., Reiterova K., Cabaj W. 2014. Detection of antibodies to Neospora caninum in moose (Alces alces): the first report in Europe. Folia Parasitologica, 61, 34-3610.14411/fp.2014.014Search in Google Scholar

Płoneczka K., Mazurkiewicz M. 2008. Seroprevalence of Neospora caninum in dogs in south-western Poland. Veterinary Parasitology, 153, 168-171. DOI: 10.1016/j.vetpar.2008.01.00710.1016/j.vetpar.2008.01.007Search in Google Scholar PubMed

Reichel M.P., Alejandra Ayanegui-Alcérreca M., Gondim L.F., Ellis J.T. 2013. What is the global economic impact of Neospora caninum in cattle - the billion dollar question. International Journal of Parasitology, 43, 133-142. DOI: 10.1016/j.ijpara. 2012.10.022Search in Google Scholar

Reiterová K., Spilovská S., Antolová D., Dubinský P. 2009. Neospora caninum, potential cause of abortions in dairy cows: the current serological follow-up in Slovakia. Veterinary Parasitology, 159, 1-6. DOI: 10.1016/j.vetpar.2008.10.00810.1016/j.vetpar.2008.10.008Search in Google Scholar PubMed

Sergeant E.S.G. 2015. Epitools epidemiological calculators. AusVet Animal Health Services and Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease. Available at: Search in Google Scholar

Thrusfield M. 2005. Veterinary epidemiology. 3rd ed., Blackwell Publishing Ltd. p. 230, 238-240, 245Search in Google Scholar

Tomczuk K., Radzikowski A.B., Studzińska M.B., Witkowska A., Puchalski A. 2007. Antibodies to Neospora caninum in sera of cows from medium and small farms near Lublin. Annales Universitatis Mariae Curie-Skłodowska Lublin-Polonia, LXII, 50-53Search in Google Scholar

Wapenaar W., Barkema H.W., Vanleeuwen J.A., McClure J.T., O’Handley R.M., Kwok O.C., Thulliez P., Dubey J.P., Jenkins M.C. 2007. Comparison of serological methods for the diagnosis of Neospora caninum infection in cattle. Veterinary Parasitology, 143, 166-173. DOI: 10.1016/j.vetpar.2006.08.007 10.1016/j.vetpar.2006.08.007Search in Google Scholar PubMed

Received: 2015-4-2
Revised: 2015-8-24
Accepted: 2015-8-31
Published Online: 2015-12-30
Published in Print: 2016-1-1

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