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Licensed Unlicensed Requires Authentication Published by De Gruyter October 24, 2016

Isolation and molecular characterization of Acanthamoeba genotypes isolated from soil sources of public and recreational areas in Iran

  • Seyed Ahmad Karamati , Maryam Niyyati EMAIL logo , Jacob Lorenzo-Morales and Zohreh Lasjerdi
From the journal Acta Parasitologica

Abstract

Pathogenic strains of Acanthamoeba are causative agents of a sight threating infection of the cornea known as Acanthamoeba keratitis. AK cases have been reported in Iran recently due to inappropriate usage of contact lens maintenance and most patients report a contact with contaminated sources such as dust, water or soil. Sixty soil samples were collected from public and recreational areas in the province of East Azerbaijan, Iran and checked for the presence of Acanthamoeba spp. Samples were cultured on non-nutrient agar plates seeded with heat killed Escherichia coli. PCR and sequencing of the DF3 region were carried out in order to genotype the isolated strains of Acanthamoeba. Thermotolerance and osmotolerance assays were performed in order to investigate the pathogenic potential of isolated Acanthamoeba strains. Acanthamoeba spp. was isolated from 41.6% of soil samples and genotyping of the strains resulted in the identification of genotypes T3, T4, T5 and T11. Most of the isolates belonging to genotypes T3 and T4 showed high pathogenic potential, indicating that they might present a potential health hazard for humans and other animals in this region. To the best of our knowledge, this is the first report on the identification of genotypes T3 and T11 from soil sources in the country.



Acknowledgement

JLM was supported by the Ramón y Cajal Subprogramme from the Spanish Ministry of Economy and Competivity RYC-2011-08863.The authors would like to thanks from Dr. Niloofar Taghipoor for her valuable comments.

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Received: 2016-1-18
Revised: 2016-5-18
Accepted: 2016-6-27
Published Online: 2016-10-24
Published in Print: 2016-12-1

© 2016 W. Stefański Institute of Parasitology, PAS

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