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Licensed Unlicensed Requires Authentication Published by De Gruyter December 28, 2016

Prevalence of three Oesophagostomum spp. from Tibetan Pigs analyzed by Genetic Markers of nad1, cox3 and ITS1

Kun Li, Yanfang Lan, Houqiang Luo, Muhammad Shahzad, Hui Zhang, Lei Wang, Lihong Zhang, Dongyu Liu, Xinyu Liu, Yanan Hao, Suolang Sizhu and Jiakui Li
From the journal Acta Parasitologica


Parasitic nematodes of Oesophagostomum spp., commonly known as ‘nodular worms’ are one of the most widely distributed and prevalent emerging zoonotic nematodes. However, little is known about the prevalence and gene characteristics of those parasites in Tibetan pigs. Therefore, a study was carried out to investigate the prevalence, isolation and identification of Oesophagostomum spp from Tibetan pigs by genetic markers of nad1,cox3 and ITS1 for the first time. The results revealed that the infection rate of O. dentatum and O. quadrispinulatum by genetic markers of nad1 was 81.13%; 35 (66.04%); the O. dentatum and O. quadrispinulatum by genetic markers cox3 was 66.04%, and O. dentatum and O. stephanostomum by genetic markers ITS1 was found to be 77.36%. Interestingly, the O. stephanostomum specie was identified and isolated from 90.48% stomach and 69.23% colon samples by genetic markers of ITS1. The present study, for the first time has described the presence and genetic characterization of Oesophagostomun spp of O. dentatum, O. quadrispinulatum and especially O. stephanostomum in Tibetan pigs from the high and remote Tibetan plateau. A public concern should be raised in terms of economical losses and severe public health problem.

  1. Conflicts of interest: The authors declare that they have no competing interests.


This study was supported by Key Science Fund of Science and Technology Agency of Tibet Autonomous Region and projects in the National Science & Technology Pillar Program during the 12thFive-year Plan Period (2012BAD3B03) and the Chinese Agricultural Research Systems (CARS-38).


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Received: 2016-6-20
Revised: 2016-8-17
Accepted: 2016-10-7
Published Online: 2016-12-28
Published in Print: 2017-3-1

© 2017 W. Stefański Institute of Parasitology, PAS

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