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Licensed Unlicensed Requires Authentication Published by De Gruyter December 28, 2016

Molecular diagnosis of Entamoeba spp. versus microscopy in the Great Cairo

Mohamed H. Roshdy, Nour M. Abd El-Kader, Marwa Ali-Tammam, Isabel Fuentes, Magdy M. Mohamed, Nabila A. El-Sheikh and Jose Miguel Rubio
From the journal Acta Parasitologica

Abstract

Amoebiasis is a human disease produced by Entamoeba histolytica which causes widespread mortality and morbidity worldwide through diarrheal disease and abscess establishment in parenchymal tissues such as liver, lung, and brain. The true prevalence of infection is unknown for most areas of the world due to the difficulty to characterise Entamoeba histolytica versus other non-pathogenic amoebas with identical morphology, as Entamoeba dispar, and Entamoeba moshkovskii. To overcome microscopy misidentification issues, we tested a nested multiplex polymerase chain reaction (PCR) and a real-time PCR on 194 stool samples collected from incoming dysentery patients in Cairo hospitals diagnosed with E. histolytica by microscopy. Nested PCR showed only 20 (10.3%) samples positive to E. histolytica and 17 (8.7%) to E. dispar. The real-time PCR detected only 19 and 11 samples positive to E. histolytica and E. dispar respectively, showing less sensitivity than the nested PCR. The data show that prevalence of E. histolytica in Cairo is lower when specific diagnosis methods are used instead of traditional microscopy, allowing to differentiate between morphologically identical human amoebas species.

Acknowledgements

This work was supported by the Spanish Agency of International Cooperation and Development (AECID) grant number A1/0355539/11 and by the AESI-ISCIII grant number PI14CIII/00014.

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Received: 2016-9-14
Revised: 2016-11-4
Accepted: 2016-11-10
Published Online: 2016-12-28
Published in Print: 2017-3-1

© 2017 W. Stefański Institute of Parasitology, PAS