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Licensed Unlicensed Requires Authentication Published by De Gruyter April 18, 2017

First report on molecular evidence of Tylodelphys cerebralis (Diplostomulum cerebralis) Chakrabarti, 1968 (Digenea: Diplostomidae) from snakehead fish Channa punctata

Anshu Chaudhary, Richa Tripathi, Shivi Gupta and Hridaya Shanker Singh
From the journal Acta Parasitologica


Freshwater snakehead fish, Channa punctata (Perciformes: Channidae) from Meerut (U.P.), India were screened for infection with metacercaria from the eye (vitreous humor) and brain (cranial cavity) and were analyzed by molecular methods using PCR and sequencing of the complete internal transcribed spacers (ITS1-5.8S-ITS2) of the ribosomal RNA gene. Recovered metacercariae from sites, vitreous humor and cranial cavity were previously diagnosed as Diplostomulum cerebralisChakrabarti, 1968 on the basis of morphological characteristics. A combination of molecular methods in this study depicts that this is a species of genus Tylodelphys Diesing, 1850 which was misinterpreted as Diplostomulum. Hence, in this study, we validated the status of T. cerebralis (=D. cerebralis) after 49 years from its original description. The present work might contribute to expand our knowledge for identification, biodiversity and taxonomy of diplostomids in Indian fishes.


The authors thank to the Head, Department of Zoology, Chaudhary Charan Singh University, Meerut, for providing laboratory facilities. This study was supported by grants from the UGC (University Grants Commission) India, under the Post Doctoral Fellowship [(F.15-191/2012 (SA-II)] to AC. The authors declare that they have no conflict of interest.


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Received: 2016-9-14
Revised: 2017-1-17
Accepted: 2017-1-18
Published Online: 2017-4-18
Published in Print: 2017-6-1

© 2017 W. Stefański Institute of Parasitology, PAS