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Redescription of Philometra margolisiMoravec, Vidal-Martínez et Aguirre-Macedo, 1995 (Nematoda: Philometridae), a gonad-infecting parasite of the red grouper Epinephelus morio (Serranidae) in the Gulf of Mexico

František Moravec, Micah D. Bakenhaster and Erin H. Leone
From the journal Acta Parasitologica

Abstract

The insufficiently known nematode species Philometra margolisiMoravec, Vidal-Martínez et Aguirre-Macedo, 1995 (Philometridae) is redescribed based on light and scanning electron microscopical (SEM) examinations of specimens collected from the gonad of the type host, the red grouper Epinephelus morio (Valenciennes) (Serranidae, Perciformes), in the northern Gulf of Mexico off Florida, USA. Also, new prevalence data for females of P. margolisi were derived from 188 fish, and a subset of these (n = 38) were used to determine prevalence and intensity of male nematodes. The male of this species was studied with SEM for the first time, which revealed some new, taxonomically important morphological features. The male posterior end had a V-shaped caudal mound, four pairs of minute adanal papillae, a pair of large papillae located posterior to the cloacal aperture and a pair of very small phasmids. The distal end of the gubernaculum is unique among all gonad-infecting species of Philometra parasitizing serranids in that its ventral surface is flat, smooth, without the usual two longitudinal grooves; the dorsal lamellate structures on the gubernaculum are also different in this species. In contrast to data in the original species description, the body length of gravid females of P. margolisi was 132–280 mm. Overall prevalence for male nematodes (76.3%) was much higher than for females (15.4%), and female nematode prevalence was higher in samples collected during host spawing season than out of season (27.1% and 3.3%, respectively).

Acknowledgements

The authors owe thanks to many colleagues at the Fish and Wildlife Research Institute (FWRI). Loanna Torrance, Jason Rock, Susan Hilber, Theresa Cody, Lauren Partridge, Susan Fogelson, Maki Tabuchi, Malcolm S. Richardson, Clark Gray, Melissa Garnett, C. J. McGuigan, and Eli Bastian provided assistance with parasite sampling in the field and laboratory. Sean Keenan, Jenna Tortorelli, Chris Stafford, Julie Vecchio, and Theresa Warner were lead scientists on research cruises and supported our shipboard efforts along with too many other members of the FWRI Fisheries Independent Monitoring Program to name individually. Theresa Cody, Bob McMichael, Deb Leffler, and Ted Switzer allocated program resources to this project. Laura Crabtree helped to identify and locate references. Thanks are also due to the staff of the Laboratory of Electron Microscopy, Institute of Parasitology, Biology Centre of the CAS, České Budějovice for their technical assistance, and to Blanka Škoríková of the same Institute for help with illustrations. This study was partly supported by the Czech Science Foundation (grant No. P505/12/G112) and the Institute of Parasitology, BC AS CR (institutional support RVO:60077344). Funding for field and laboratory sampling was provided to FWRI by the US Department of the Interior US Fish and Wildlife Service (USFWS) Federal Sportfish Restoration Grants F-72-12, 13, 14, 15, and16, and F121AF00878, F13AF00529, F14AF00955, F15AF00893, F16AF00544, F-43-24, F-43-26, F12AF00222, F13AF00325, F14AF00328, and F16AF00413, and by National Oceanic and Atmospheric Administration (NOAA) grants NA06NMF4350009, NA08NMF4330403, and NA11NMF45 40116. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the opinions or policies of the U.S. government, the USFWS, or NOAA.

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Received: 2016-11-16
Revised: 2017-1-20
Accepted: 2017-1-26
Published Online: 2017-4-18
Published in Print: 2017-6-1

© 2017 W. Stefański Institute of Parasitology, PAS