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The systematic position and structure of the genus Leyogonimus Ginetsinskaya, 1948 (Platyhelminthes: Digenea) with comments on the taxonomy of the superfamily Microphalloidea Ward, 1901

Gerard Kanarek, Grzegorz Zaleśny, Jiljí Sitko and Vasyl V. Tkach
From the journal Acta Parasitologica


The systematic position, phylogenetic relationships and composition of the genus Leyogonimus Ginetsinskaya, 1948 have always been uncertain. In the present study, we investigate the taxonomic position and phylogenetic relationships between the type-species L. polyoon (Linstow, 1887) and L. postgonoporus (Neiland, 1951) (previously classified as Macyella), based on newly obtained partial sequences of the nuclear large ribosomal subunit DNA. To test some of the previously proposed systematic arrangements, we have also sequenced specimens of Stomylotrema vicarium Braun, 1901 and Phaneropsolus sp. Our results clearly demonstrate that both L. polyoon and L. postgonoporus belong to the family Pleurogenidae Looss, 1899 within the superfamily Microphalloidea. Thus, the Leyogonimidae Dollfus, 1951 should be recognized as a synonym of the Pleurogenidae. Leyogonimus polyoon clearly constitutes a separate, sister branch to the clade consisting of Collyricloides massanaeVaucher, 1969 and L. postgonoporus. Based on these results, we resurrect the genus MacyellaNeiland, 1951 with type-species M. postgonoporus. Besides, Collyricloides Vaucher, 1968 is synonymized with Macyella resulting in new combination Macyella massanae (Vaucher, 1968) comb. nov. Molecular phylogenetic analysis has demonstrated the lack of a close phylogenetic relationships between Stomylotema vicarium and Leyogonimus previously placed by several authors into the family Stomylotrematidae Poche, 1925. The status of the Phaneropsolidae Mehra, 1935 as independent family was confirmed with the addition of the newly sequenced Phaneropsolus sp. from China.


We thank Eric Pulis (Institute for Marine Mammal Studies, Gulfport, Mississippi, USA) for his useful comments on the draft of the manuscript, Kaylyn Patitucci (Univerity of North Dakota) for collecting Stomylotrema vicarium in the field and help with its laboratory processing, and Sara Bush (University of Utah, Salt Lake City, USA) for providing frozen intestine of Indian Paradise Flycatcher that yielded specimens of Phaneropsolus sp. This study was partly supported by the Polish Ministry of Science and Higher Education grant IP2010 016570 for GK and the grant DEB 1120734 from the National Science Foundation (USA) to VVT.


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Received: 2017-3-17
Revised: 2017-5-2
Accepted: 2017-5-5
Published Online: 2017-7-5
Published in Print: 2017-9-26

© 2017 W. Stefański Institute of Parasitology, PAS