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Licensed Unlicensed Requires Authentication Published by De Gruyter July 5, 2017

Leishmania infantum is present in vaginal secretions of naturally infected bitches at lower levels in oestrogenized bitches than in non-oestrogenized bitches

Alinne Gonçalves Magro, Viviane Pedersoli Assis, Lucélia Coimbra Silva, Wagner Luiz Tafuri, Soraia Oliveira Silva, Maria Norma Melo and Guilherme Ribeiro Valle
From the journal Acta Parasitologica

Abstract

Naturally Leishmania infantum infected bitches were divided into oestrogenized (n = 11) and non-oestrogenized (n = 6) groups. Vaginal secretions were collected for polymerase chain reaction (PCR), and vulval, vaginal and uterine tissues were collected for the immunohistochemical (IHQ) identification of L. infantum. Parasite DNA was identified in vaginal secretions of non-oestrogenized (41.8%) and oestrogenized (18.2%) bitches (P<0.05; Fisher’s Exact test). IHQ was positive in vulvar dermis (23.5%) and vaginal mucosa (17.7%) but negative in endometrium of all bitches. Poor association between positive vaginal secretion PCR and tissue IHQ (Kappa index) were observed. The results showed that genital secretions are a potential source for dog contamination.

Acknowledgments

Funding: This work was supported by the Fundo de Incentivo á Pesquisa da Pontificia Universidade Católica de Minas Gerais (FIP/PUC Minas) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico [grant number 2007/2399- S1], Brazil.

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Received: 2016-12-29
Revised: 2017-5-2
Accepted: 2017-5-8
Published Online: 2017-7-5
Published in Print: 2017-9-26

© 2017 W. Stefański Institute of Parasitology, PAS