Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter July 5, 2017

Redescription of two species of Thaparocleidus (Monogenea: Dactylogyridae), with the description of T. armillatus sp. n. from Wallago attu and a phylogenetic analysis based on 18S rDNA sequences

Chandni Verma, Anshu Chaudhary and Hridaya Shanker Singh
From the journal Acta Parasitologica


The phylogenetic studies on monogeneans of the genus Thaparocleidus parasitizing W. attu in India was inferred from 18S rDNA gene data. Out of species of Thaparocleidus, one new, T. armillatus sp. n., is described herein which distinguished from its congener species in the shapes and sizes of sclerotized structures and rest of the two species, T. indicus and T. wallagonius are redescribed morphologically as well as validated and confirmed by molecular means also. Besides the above, T. gangus was also included in the molecular analysis as it is recently described on the basis of morphology only. The phylogenetic tree was also reconstructed in order to elucidate the taxonomic status of Thaparocleidus armillatus sp. n., T. indicus, T. wallagonius and T. gangus. This research reports for the first time, the molecular phylogenetic analysis of 18S rDNA gene for four species belonging to the genus Thaparocleidus from W. attu collected off the River Ganga in India. In phylogram, all four species of Thapaocleidus come in a single clade that confirmed their monophyletic status. A very low interspecific variability was observed in T. armillatus sp. n. and T. wallagonius suggesting that these species are highly similar in morphology. Correspondingly, T. indicus and T. gangus also showed low interspecific variability which too signifies their morphological similarities in their sclerotized structures. Our result suggested that all four species taken in the present investigation from India are monophyletic and it is also observed that morphological similarities in the sclerotized structures are well correlated with phylogeny.


The authors thank to the Head, Department of Zoology, Chaudhary Charan Singh University, Meerut, for providing laboratory facilities. Special thanks to Prof. Jean Mariaux, Department of Invertebrates, Natural History Museum, Geneva, Switzerland for reading the pre-submission draft of manuscript and his valuable suggestions to improve it. This study was partially supported by grants from the UGC (University Grants Commission) India, under the Senior Research Fellowship [RGNF; F.14-2(SC)/2010 (SA-III)] to CV and Post Doctoral Fellowship [(F.15-191/2012 (SA-II)] to AC. The authors declare that they have no conflict of interest.


Akhmerov A.K. 1964. On the evolution of the median attachment apparatus of the monogenetic suborder Dactylogyrinea. Trudy Gel’minthologicheskoi Laboratorii, 14, 69–79 (In Russian)Search in Google Scholar

Agrawal N., Sharma R. 1988. On a new monogenea Neomurraytrema lucknowensis sp. nov. from Mystus vittatus Bloch). Indian Journal of Parasitology, 12, 61–63Search in Google Scholar

Agrawal N., Vishwakarma, P., Shukla R., Devak A. 2005. A note on surface topography of Thaparocleidus indicus (Kulkarni, 1969) Lim, 1966 on Wallago attu (Bloch and Schn.). National Journal of Life Science, 2, 131–132Search in Google Scholar

Caballero Y.C.E., Bravo-Hollis, M. 1961. Trematodes de peces de aguas Mexicanas del Pacifico. XVIII. Un nuevo género y una nueva especie de Monogenoidea Byjovskii, 1937. Anales del Instituto de Biología, 31, 197–205. (In Spanish)Search in Google Scholar

Chaudhary A., Singh H.S. 2012. Phylogenetic study of nine species of freshwater monogeneans using secondary structure and motif prediction from India. Bioinformation, 8, 862–86910.6026/97320630008862Search in Google Scholar

Chaudhary A., Singh H.S. 2012. Description of two new species of the genus Thaparocleidus Jain, 1952 (Monogenea, Dactylogyridae) from freshwater fish in India: morphological and molecular phylogenetic evidence. Journal of Helminthology, 87, 160–17310.1017/S0022149X12000119Search in Google Scholar

Chaudhary A., Verma C., Varma M., Singh H.S. 2014. Identification of Thaparocleidus caecus (Monogenea: Dactylogyridae) using morphological and molecular tools: a parasite invasion in Indian freshwater. Bioinvasions Records, 3, 195–20010.3391/bir.2014.3.3.10Search in Google Scholar

Dubey A., Gupta A.K., Agarwal S.M. 1992. Studies on monogenean parasites in freshwater fishes at Raipur. VIII. Validity of Parancylodiscoides Akhmerov, 1964 (Silurodiscoides Gusev, 1974) and two new species from Raipur. Indian Journal of Helminthology, 44, 9–16Search in Google Scholar

Froese R., Pauly D. 2002. Fish Base: World Wide Web electronic publication. www.fishbase.orgSearch in Google Scholar

Gusev A.V. 1973. Monogeneans of freshwater fishes of India and an analysis of the world fauna of the group. Abstract of Doctor of Biological Science Thesis, Leningrad: Akademiya Nauk, pp. 31Search in Google Scholar

Gusev A.V. 1976. Freshwater Indian Monogenoidea. Principles of systematics, analysis of the world faunas and their evolution. Indian Journal of Helminthology, 25 and 26 (1973 and 1974), 1–241Search in Google Scholar

Hall T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98Search in Google Scholar

Jain S.L. 1952. Monogenea of Indian freshwater fishes. II. Thaparocleidus wallagonius n.g. and n. sp. (subfamily Tetraonchinae) from the gills of Wallagonia attu (Bloch) from Lucknow. Indian Journal of Helminthology, 4, 43–48Search in Google Scholar

Kulkarni T. 1969. On a new species Ancylodiscoides Yamaguti, 1937 from the fish Wallagonia attu (Bloch) in Hyderabad, A.P. Zoologische Anzeiger, 182, 461–465Search in Google Scholar

Lim L.H.S. 1996. Thaparocleidus Jain, 1952, the senior synonym of Silurodiscoides Gussev, 1976 (Monogenea: Ancylodiscoidinae). Systematic Parasitology, 35, 207–21510.1007/BF00009640Search in Google Scholar

Lim L.H.S. 1998. Diversity of monogeneans in Southeast Asia. International Journal of Parasitology, 28, 1495–151510.1016/S0020-7519(98)00061-7Search in Google Scholar

Lim L.H.S., Lerssutthichawal T. 1996. Monogeneans from Wallago attu (Bloch and Schneider, 1802) of Thailand. Raffles Bulletin of Zoology, 44, 287–300Search in Google Scholar

Lim L.H.S., Timofeeva T.A., Gibson D.I. 2001. Dactylogyridean monogeneans of the siluriform fishes of the Old World. Systematic Parasitology, 50, 159–19710.1023/A:1012237801974Search in Google Scholar

Littlewood D.T.J., Olson P.D. 2001. Small subunit rDNA and the phylum Platyhelminthes: signal, noise, conflict and compromise. In: (Eds Littlewood, D.T.J., Bray, R.A.) Interrelationships of the Platyhelminthes. Taylor and Francis, London, U.K., pp. 262–278Search in Google Scholar

Mendoza-Palmero C.A., Blasco-Costa I., Scholz T. 2015. Molecular phylogeny of Neotropical monogeneans (Platyhelminthes: Monogenea) from catfishes (Siluriformes). Parasites & Vectors, 8, 16410.1186/s13071-015-0767-8Search in Google Scholar PubMed PubMed Central

Milne I., Lindner D., Bayer M., Husmeier D., McGuire G., Marshall D.F., Wright F. 2009. TOPALi v2: a rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops. Bioinformatics, 25, 126–12710.1093/bioinformatics/btn575Search in Google Scholar PubMed PubMed Central

Pandey K.C., Agarwal A. 1989. On a new monogenetic trematoda from Wallago attu (Bl.). Indian Journal of Helminthology (N.S.), 5, 51–53Search in Google Scholar

Pandey K.C., Agarwal N. 2008. An encyclopaedia of Indian monogenoidea. Vitasta Publishing Pvt. Ltd., New Delhi, India, pp. 522Search in Google Scholar

Pandey K.C., Mehta T. 1986. Studies on some new monogenetic trematodes of Wallago attu (Bloch) at Meerut, Uttar Pradesh, India. In: Proceedings of the Indian National Convocation for Young Scientists, 114–132Search in Google Scholar

Posada D., Crandall K.A. 1998. Modeltest: Testing the model of DNA substitution. Bioinformatics, 14, 817–81810.1093/bioinformatics/14.9.817Search in Google Scholar PubMed

Šimková A., Serbielle C., Pariselle A., Vanhove M.P.M., Morand S. 2013. Speciation in Thaparocleidus (Monogenea: Dactylogyridae) parasitizing Asian pangasiid catfishes. BioMed Research International, 353956. 10.1155/2013/353956Search in Google Scholar PubMed PubMed Central

Singh H.S., Sharma B. 1992. Wallagotrema indicus n. sp., a new monogenean from the freshwater shark Wallago attu (Bloch and Schn). Functional Development and Morphology, 2, 85–88Search in Google Scholar

Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular evolution genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–272910.1093/molbev/mst197Search in Google Scholar PubMed PubMed Central

Thompson J.D., Higgins D.G., Gibson T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–468010.1093/nar/22.22.4673Search in Google Scholar PubMed PubMed Central

Tripathi Y.R. 1959. Monogenetic trematodes from fishes of India. Indian Journal of Helminthology, 9, 1–149Search in Google Scholar

Van de Peer Y., De Rijk P., Wuyts J., Winkelmans T., De Wachter R. 2000. The European Small Subunit Ribosomal RNA database. Nucleic Acids Research, 28, 175–176.10.1093/nar/28.1.175Search in Google Scholar PubMed PubMed Central

Verma C., Chaudhary A., Singh H.S. 2015. Morphological redescription and first molecular analyses of two species of Mizelleus (Monogenea, Dactylogyridae) infecting the Indian Gangetic shark, Wallago attu. Journal of Helminthology 16, 1–11. 10.1017/S0022149X15000814Search in Google Scholar PubMed

Verma C., Chaudhary A., Singh H.S. 2016. Thaparocleidus gangus sp. nov. (Monogenea: Dactylogyridae) from gill filaments of Wallago attu Bloch and Schn., 1801, India. Turkish Journal of Zoology, 40: 758–764. 10.3906/zoo-1507-31Search in Google Scholar

Received: 2016-10-21
Revised: 2017-5-12
Accepted: 2017-5-18
Published Online: 2017-7-5
Published in Print: 2017-9-26

© 2017 W. Stefański Institute of Parasitology, PAS