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Redescription of two species of Thaparocleidus (Monogenea: Dactylogyridae), with the description of T. armillatus sp. n. from Wallago attu and a phylogenetic analysis based on 18S rDNA sequences

Chandni Verma, Anshu Chaudhary and Hridaya Shanker Singh
From the journal Acta Parasitologica

Abstract

The phylogenetic studies on monogeneans of the genus Thaparocleidus parasitizing W. attu in India was inferred from 18S rDNA gene data. Out of species of Thaparocleidus, one new, T. armillatus sp. n., is described herein which distinguished from its congener species in the shapes and sizes of sclerotized structures and rest of the two species, T. indicus and T. wallagonius are redescribed morphologically as well as validated and confirmed by molecular means also. Besides the above, T. gangus was also included in the molecular analysis as it is recently described on the basis of morphology only. The phylogenetic tree was also reconstructed in order to elucidate the taxonomic status of Thaparocleidus armillatus sp. n., T. indicus, T. wallagonius and T. gangus. This research reports for the first time, the molecular phylogenetic analysis of 18S rDNA gene for four species belonging to the genus Thaparocleidus from W. attu collected off the River Ganga in India. In phylogram, all four species of Thapaocleidus come in a single clade that confirmed their monophyletic status. A very low interspecific variability was observed in T. armillatus sp. n. and T. wallagonius suggesting that these species are highly similar in morphology. Correspondingly, T. indicus and T. gangus also showed low interspecific variability which too signifies their morphological similarities in their sclerotized structures. Our result suggested that all four species taken in the present investigation from India are monophyletic and it is also observed that morphological similarities in the sclerotized structures are well correlated with phylogeny.

Acknowledgements

The authors thank to the Head, Department of Zoology, Chaudhary Charan Singh University, Meerut, for providing laboratory facilities. Special thanks to Prof. Jean Mariaux, Department of Invertebrates, Natural History Museum, Geneva, Switzerland for reading the pre-submission draft of manuscript and his valuable suggestions to improve it. This study was partially supported by grants from the UGC (University Grants Commission) India, under the Senior Research Fellowship [RGNF; F.14-2(SC)/2010 (SA-III)] to CV and Post Doctoral Fellowship [(F.15-191/2012 (SA-II)] to AC. The authors declare that they have no conflict of interest.

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Received: 2016-10-21
Revised: 2017-5-12
Accepted: 2017-5-18
Published Online: 2017-7-5
Published in Print: 2017-9-26

© 2017 W. Stefański Institute of Parasitology, PAS