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Licensed Unlicensed Requires Authentication Published by De Gruyter October 15, 2017

Prevalence and molecular characterization of bovine Cryptosporidium from dairy cows in Northern Thailand

Tawin Inpankaew, Tawisa Jiyipong, Chainirun Sunanta, Chanya Kengradomkij, Nongnuch Pinyopanuwat and Sathaporn Jittapalapong
From the journal Acta Parasitologica

Abstract

Cryptosporidiosis is a common protozoan infection in humans and domestic animals. It is the culprit for significant neonatal morbidity in cattle as well as weight loss and delayed growth, which leads to large economic losses in the farming industry. Furthermore, bovine Cryptosporidium is also a principal source of human Cryptosporidium infections. The purpose of this study is to determine prevalence and genotype of Cryptosporidium spp. from feces of dairy cows from the northern parts of Thailand (Chiang Rai, Chiang Mai, and Lumpang provinces). A total of 500 fecal samples were collected directly from the rectum and they were examined for potential presence of Cryptosporidium infection by using tests such as DMSO-modified acid fast stain, immunofluorescence antibody test (IFAT) and polymerase chain reaction (PCR). The prevalence of Cryptosporidium spp. was 5% by DMSO-modified acid fast stain, 7% by IFAT and 7.6% by PCR respectively. The main genotypes of Cryptosporidium spp. identified were Cryptosporidium parvum and Cryptosporidium bovis. Therefore, as a result of this study, it can be said that, due to the potential cross-species transmission of Cryptosporidium parvum, infected dairy cows may pose a potential zoonotic risk to humans.

Acknowledgments

The authors would like to thank the provincial veterinary officers in the Department of Livestock Development in Chiang Rai, Chiang Mai and Lampang provinces for their help in collecting stool samples. We also thank the dairy cow owners for cooperating in this study. This project was funded by Kasetsart University Research Development Institute (KURDI), Thailand.

References

Al Mawly, J., Grinberg, A., Prattley, D., Moffat, J., French, N. 2015. Prevalence of endemic enteropathogens of calves in New Zealand dairy farms. New Zealand Veterinary Journal, 63, 147–152. 10.1080/00480169.2014.966168Search in Google Scholar PubMed

Amer S, Zidan S, Adamu H, Ye J, Roellig D, Xiao L, Feng Y. 2013. Prevalence and characterization of Cryptosporidium spp. in dairy cattle in Nile River delta provinces, Egypt. Experimental Parasitology, 135, 518–523. 10.1016/j.exppara.2013.09.002Search in Google Scholar PubMed

Delafosse, A., Chartier, C., Dupuy, M.C., Dumoulin, M., Pors, I., Paraud, C. 2015. Cryptosporidium parvum infection and associated risk factors in dairy calves in western France. Preventive Veterinary Medicine, 118, 406–412. 10.1016/j.prevetmed.2015.01.005Search in Google Scholar PubMed PubMed Central

Fayer, R. 2004. Cryptosporidium: a water-borne zoonotic parasite. Veterinary Parasitology, 126, 37–5610.1016/j.vetpar.2004.09.004Search in Google Scholar PubMed

Feng, Y., Ortega, Y., He, G., Das, P., Xu, M., Zhang, X., et al. 2007. Wide geographic distribution of Cryptosporidium bovis and the deerlike genotype in bovines. Veterinary Parasitology, 144, 1–910.1016/j.vetpar.2006.10.001Search in Google Scholar PubMed

Geurden T, Berkvens D, Martens C, Casaert S, Vercruysse J, Claerebout E. 2007. Molecular epidemiology with subtype analysis of Cryptosporidium in calves in Belgium. Parasitology, 134, 1981–710.1017/S0031182007003460Search in Google Scholar PubMed

Hisamuddin, N.H., Hashim, N., Soffian, S.N., Amin, M.H., Wahab, R.A., Mohammad, M., et al. 2016. Identification of Cryptosporidium from dairy cattle in Pahang, Malaysia. Korean Journal of Parasitology, 54, 197–200. 10.3347/kjp.2016.54.2.197Search in Google Scholar PubMed PubMed Central

Inpankaew, T., Jiyipong, T., Pinyopanuwat, N., Chimnoi, W., Thompson, R.C., Jittapalapong, S. 2010. Prevalence and genotyping of Cryptosporidium spp. from dairy cow fecal samples in western Thailand. Southeast Asian Journal of Tropical Medicine and Public Health, 41, 770–775Search in Google Scholar

Jittapalapong, S., Pinyopanuwat, N., Chimnoi, W., Siripanth, C., Stich, R.W. 2006. Prevalence of Cryptosporidium among dairy cows in Thailand. Annals of the New York Academy of Sciences, 1081, 328–33510.1196/annals.1373.045Search in Google Scholar PubMed

Keshavarz, A., Haghighi, A., Athari, A., Kazemi, B., Abadi, A., Nazemalhosseini Mojarad, E. 2009. Prevalence and molecular characterization of bovine Cryptosporidium in Qazvin province, Iran. Veterinary Parasitology, 160, 316–318. 10.1016/j.vetpar.2008.11.008Search in Google Scholar PubMed

Khan SM, Debnath C, Pramanik AK, Xiao L, Nozaki T, Ganguly S. 2010. Molecular characterization and assessment of zoonotic transmission of Cryptosporidium from dairy cattle in West Bengal, India. Veterinary Parasitology, 171, 41–47. 10.1016/j.vetpar.2010.03.008Search in Google Scholar PubMed

O’Handley, R.M., Olson, M.E., Fraser, D., Adams, P., Thompson, R.C. 2000. Prevalence and genotypic characterisation of Giardia in dairy calves from Western Australia and Western Canada. Veterinary Parasitology, 90, 193–20010.1016/S0304-4017(00)00235-1Search in Google Scholar PubMed

Mahfouz, M.E., Mira, N., Amer, S. 2014. Prevalence and genotyping of Cryptosporidium spp. in farm animals in Egypt. Journal of Veterinary Medical Science, 76, 1569–1575. 10.1292/jvms.14-0272Search in Google Scholar PubMed PubMed Central

McDonald, V. 2000. Host cell-mediated responses to infection with Cryptosporidium. Parasite Immunology, 22, 597–60410.1046/j.1365-3024.2000.00343.xSearch in Google Scholar PubMed

Meireles, M.V., de Oliveira, F.P., Teixeira, W.F., Coelho, W.M., Mendes, L.C. 2011. Molecular characterization of Cryptosporidium spp. in dairy calves from the state of São Paulo, Brazil. Parasitology Research, 109, 949–951. 10.1007/s00436-011-2336-1Search in Google Scholar PubMed

Nuchjangreed, C., Boonrod, K., Ongerth, J., Karanis, P. 2008. Prevalence and molecular characterization of human and bovine Cryptosporidium isolates in Thailand. Parasitology Research, 103, 1347–1353. 10.1007/s00436-008-1139-5Search in Google Scholar PubMed

Smith, R.P., Clifton-Hadley, F.A., Cheney, T., Giles, M. 2014. Prevalence and molecular typing of Cryptosporidium in dairy cattle in England and Wales and examination of potential on-farm transmission routes. Veterinary Parasitology, 204, 111–119. 10.1016/j.vetpar.2014.05.022Search in Google Scholar PubMed PubMed Central

Venu, R., Latha, B.R., Basith, S.A., Raj, G.D., Sreekumar, C., Raman, M. 2012. Molecular prevalence of Cryptosporidium spp. in dairy calves in Southern states of India. Veterinary Parasitology, 188, 19–24. 10.1016/j.vetpar.2012.02.025Search in Google Scholar PubMed

Wegayehu, T., Karim, R., Anberber, M., Adamu, H., Erko, B., Zhang, L., Tilahun. G. 2016. Prevalence and genetic characterization of Cryptosporidium species in dairy calves in central Ethiopia. PLoS One, 11, e0154647. 10.1371/journal.pone.0154647Search in Google Scholar PubMed PubMed Central

Xiao, L., Escalante, L., Yang, C., Sulaiman, I., Escalante, A.A., Montali, R.J., et al. 1999. Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Applied and Environmental Microbiology, 65, 1578–158310.1128/AEM.65.4.1578-1583.1999Search in Google Scholar PubMed PubMed Central

Zhang, X.X., Tan, Q.D., Zhou, D.H., Ni, X.T., Liu, G.X., Yang, Y.C., Zhu, X.Q. 2015. Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle, northwest China. Parasitology Research, 114, 2781-2787. 10.1007/s00436-015-4537-5Search in Google Scholar PubMed

Received: 2017-3-14
Revised: 2017-8-1
Accepted: 2017-8-2
Published Online: 2017-10-15
Published in Print: 2017-12-20

© 2017 W. Stefański Institute of Parasitology, PAS