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Licensed Unlicensed Requires Authentication Published by De Gruyter October 15, 2017

Sero-detection of Toxocara canis infection in human with T.canis recombinant arginine kinase, cathepsin L-1 and TES-26 antigens

Anju Varghese, Opinder K. Raina, Dinesh Chandra, Bijay R. Mirdha, Naresh H. Kelawala, Jayesh B. Solanki, Niranjan Kumar, Reghu Ravindran, Anandanarayanan Arun, Ajayta Rialch, Hniang Lalrinkima, Rohan N. Kelawala and Subhamoy Samanta
From the journal Acta Parasitologica


Three recombinant antigens viz. arginine kinase, cathepsin L-1 and TES-26 of Toxocara canis were expressed in Escherichia coli and evaluated for their potential in the detection of T. canis larval infection in human in immunoglobulin G-enzyme linked immunosorbent assay (IgG-ELISA). Results of the IgG-ELISA with the above recombinant antigens were confirmed with commercially available IgG detection kit for T. canis infection used as a standard test. All three recombinant antigens were 100% sensitive in the detection of positive cases (n = 6) of T. canis infection in human and were screened for their cross-reactivity in human patients with history of Toxoplasma gondii, Plasmodium vivax, Entamoeba histolytica, hydatid and hookworm infections. The recombinant TES-26 antigen showed higher specificity and cross-reacted with T. gondii infection sera only. However, arginine kinase and cathepsin L-1 recombinant antigens showed cross-reactions with sera of patients infected with T. gondii, P. vivax and E. histolytica but not with the patient sera infected with hydatid and hookworm. These results show that recombinant TES-26 is a potential diagnostic candidate antigen for human toxocarosis caused by migrating T. canis larvae.


The authors are thankful to the Director, ICAR-Indian Veterinary Research Institute, Izatnagar and Dean, Vanbandhu College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, Gujarat for providing facilities for carrying out the research work. Present research work was carried out under the approved sub-component of the Outreach project on Zoonosis, ICAR, New Delhi.


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Received: 2017-6-17
Revised: 2017-7-31
Accepted: 2017-8-1
Published Online: 2017-10-15
Published in Print: 2017-12-20

© 2017 W. Stefański Institute of Parasitology, PAS

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