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Licensed Unlicensed Requires Authentication Published by De Gruyter October 15, 2017

Genotyping of Acanthamoeba spp. from water sources from Northwestern Iran

Ali Haniloo, Ali Pezeshki, Abbas Mahmmodzadeh and Elnaz Kadkhodamohammadi
From the journal Acta Parasitologica


Acanthamoeba spp. are free-living amoebae which are ubiquitously distributed worldwide and can be found in the wide range of environments, particularly in various types of water sources, where they able to cause important health problems. In the present study, cultures containing Acanthamoeba from water samples were obtained from our earlier survey. For an analysis of the genetic pattern of Acanthamoeba isolates, DNA sequencing of nuclear small-subunit rRNA gene (18S rRNA or Rns) was applied. A phylogenetic analyses of the isolates displayed that all of them were belonged to the potentially pathogenic T4 genotype. This investigation provides further evidence that the T4 genotype is the most prevalent in water samples and demonstrates that there is a need for taking more consideration to water sources in order to prevent complications associated with pathogenic Acanthamoeba spp.


The authors are extremely grateful to all of the technicians in Parasitology and Mycology Department of, School of Medicine, Zanjan University of Medical Sciences who helped to carry out this study. This investigation was supported by a grant from The Research Vice-Chancellery of Zanjan University of Medical Sciences (No. A-12-942-1).


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Received: 2017-3-17
Revised: 2017-8-4
Accepted: 2017-8-8
Published Online: 2017-10-15
Published in Print: 2017-12-20

© 2017 W. Stefański Institute of Parasitology, PAS

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