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Licensed Unlicensed Requires Authentication Published by De Gruyter October 15, 2017

Humoral response of mice infected with Toxocara canis following different infection schemes

Jan Novák, Lucie Panská, Tomáš Macháček, Libuše Kolářová and Petr Horák
From the journal Acta Parasitologica


The study was focused on the dynamics of humoral response to Toxocara canis excretory-secretory antigens (TES antigens) in mice experimentally infected by T. canis L3 larvae in different ways. In particular, we compared the effect of infection with two doses of 1000 larvae vs. repeated infections with a low number of larvae (daily infection with 10 larvae and weekly infection with 100 larvae in the course of 22 weeks). In ELISA, all infections, including both schemes with lower larval doses, elicited significant antibody response. Elevated levels of total IgE and TES-antigen-specific IgM were detected on day 12 after the first infection, followed by IgG and IgG1, and later by IgG3, IgG2a and IgG2b; specific IgE response was not detected. It seems that the high levels of IgM and IgG1 represent the best markers of infection. In addition, gradual increase of IgG2a and IgG2b could help in determination of the infection course. As a byproduct of our work, a new method of infection by repeated drinking of larvae was introduced; it minimizes the pain and discomfort for the experimental mice.

  1. Disclosures: The authors declare no conflict of interest.


As for Jan Novák and Libuše Kolářová, the study was financially supported by Charles University (GAUK 32915, SVV 266508, and SVV 260369). As for Petr Horák, Tomáš Macháček and Lucie Panská, the study was financially supported by Charles University (PROGRES Q43, UNCE 204017, and SVV 244260432/2017).


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Received: 2017-6-8
Revised: 2017-8-2
Accepted: 2017-8-11
Published Online: 2017-10-15
Published in Print: 2017-12-20

© 2017 W. Stefański Institute of Parasitology, PAS