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Licensed Unlicensed Requires Authentication Published by De Gruyter April 13, 2018

A new species of Cacatuocotyle (Monogenea, Dactylogyridae) parasitizing Astyanax spp. (Characiformes, Characidae) from Brazil, including molecular data and a key to species identification

Aline Cristina Zago, Lidiane Franceschini, Maria Isabel Müller and Reinaldo José da Silva
From the journal Acta Parasitologica

Abstract

The present study describes Cacatuocotyle papilionis n. sp. (Monogenea, Dactylogyridae) from the skin of the characid fishes Astyanax lacustris (Lütken, 1875) (=Astyanax altiparanae Garutti & Britski, 2000) and Astyanax fasciatus (Cuvier, 1819) (Characiformes, Characidae) from the Southeast of Brazil, supported by morphological and molecular data. The new species differs from all congeners, mainly due to the morphology of the ventral bar (resembling a butterfly), accessory piece, and the number of rings of the male copulatory organ (MCO), comprising a coiled tube with 4.5-5.5 counterclockwise rings. The first molecular data for this monogenean genus is provided in this study, using the partial sequences of the ribosomal gene (28S), as well as providing an identification key to the species.

Acknowledgments

This work was supported by FAPESP - Fundação de Amparo á Pesquisa do Estado de São Paulo (São Paulo Research Foundation) (A.C.Z., grant numbers 2011/23588-8, 2015/11542-4, 2016/07829-9; L.F., grant number 2012/07850-7), CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (the Coordination for the Improvement of Higher Education Personnel) and CAPES/PNPD (L.F.: 17/2016); AUX-PE-PNPD (M.I.M., grant number 3005/2010), the Young Researcher Program PROPE-UNESP (M. I. M., grant number 02/2016); FUNEP - Fundação de Apoio a Pesquisa, Ensino e Extensão (the Research, Teaching and Extension Support Foundation) (grant number 1.01852/2011); R.J.S. was supported by CNPq (grant number 309125/2017-0) and CNPq-PROTAX (grant number 440496/2015-2) / FAPESP (grant number 2016/50377-1). We are grateful to Edmir Daniel Carvalho (in memoriam), Sandro Geraldo de Castro Britto, Diogo Freitas Souza, Marcos Gomes Nogueira, Duke Energy and CELAN (Central Elétrica Anhanguera) for logistical support during the collections.

References

Acosta A.A., Franceschini L., Zago A.C., Scholz T., Silva R.J. 2017. Six new species of Heteropriapulus (Monogenea: Dactylogyridae) from South American fishes with an amended diagnosis to the genus. Zootaxa, 4290, 459–482. 10.11646/zootaxa.4290.3.3Search in Google Scholar

Acosta A.A., Scholz T., Blasco-Costa I., Alves P.V., Silva R.J. 2018. A new genus and two new species of dactylogyrids monogeneans from gills of Neotropical catfishes (Siluriformes: Doradidae and Loricariidae). Parasitology International, 67, 4–12. 10.1016/j.parint.2017.09.012Search in Google Scholar PubMed

Behrmann-Godel J., Roch, S., Brinker, A. 2014. Gill worm Ancyrocephalus percae (Ergens, 1966) outbreak negatively impacts the Eurasian perch Perca fluviatilis L. stock of Lake Constance, Germany. Journal of Fish Diseases, 37, 925–930, doi:10.1111/jfd.12178Search in Google Scholar PubMed

Boeger W.A., Domingues M.V., Kritsky D.C. 1997. Neotropical Monogenoidea. 32. Cacatuocotyle paranaensis n. g., n. sp. (Dactylogyridae, Ancyrocephalinae) from Characidium spp. (Teleostei, Characidae) from the State of Paraná, Brazil. Systematic Parasitology, 36, 75–78. 10.1023/A:1005796027406Search in Google Scholar

Buchmann K., Lindenstrøm T. 2002. Interactions between monogenean parasites and their fish hosts. International Journal for Parasitology, 32, 309–319. 10.1016/S0020-7519(01)00332-0Search in Google Scholar PubMed

Bush A.O., Lafferty K.D., Lotz J.M., Shostak A.W. 1997. Parasitology meets ecology on its own terms: Margolis et al. revisited. Journal of Parasitology, 83, 575–58310.2307/3284227Search in Google Scholar

Cohen S.C., Justo M.C., Kohn A. 2013. South American Monogenoidea parasites of fishes, amphibians and reptiles. Oficina de livros, Rio de Janeiro, pp. 663Search in Google Scholar

Cunningham C.O., Mo T.A., Collins C.M., Buchmann K., Thiery R., Blanc G., Lautraite A. 2001. Redescription of Gyrodactylus teuchis Lautraite, Blanc, Thiery, Daniel & Vigneulle, 1999 (Monogenea: Gyrodactylidae); a species identified by ribosomal RNA sequence. Systematic Parasitology, 48, 141–150. 10.1023/A:1006407428666Search in Google Scholar PubMed

Eiras J.C., Takemoto R.M., Pavanelli G.C. 2010. Diversidade dos parasitas de peixes de água doce do Brasil. Clichetec, Maringá, pp. 333Search in Google Scholar

Eschmeyer W.N., Fricke R., van der Laan R. (Eds) 2016. Catalog of fishes: genera, species, references. Available at: <http://re-searcharchive.calacademy.org/research/ichthyology/catalog/SpeciesByFamily.asp> Accessed in: June, 2017Search in Google Scholar

Euzet L., Combes C. 1998. The selection of habitats among the monogenea. International Journal for Parasitology, 28, 1645–1652. 10.1016/S0020-7519(98)00062-9Search in Google Scholar PubMed

Fehlauer-Ale K.H., Littlewood D.T.J. 2011. Molecular phylogeny of Potamotrygonotyle (Monogenea, Monocotylidae) challenges the validity of some of its species. Zoologica Scripta, 40, 638–65810.1111/j.1463-6409.2011.00496.xSearch in Google Scholar

Franceschini L., Zago A.C., Müller M.I., Francisco C.J., Takemoto R.M., Silva, R.J. 2017. Morphology and molecular characterization of Demidospermus spirophallus n. sp., D. prolixus n. sp. (Monogenea: Dactylogyridae) and a redescription of D. anus in siluriform catfish from Brazil. Journal of Helminthology. 10.1017/S0022149X17000256Search in Google Scholar PubMed

Gallas M., Calegaro-Marques C., Amato S.B. 2014. A new species of Cacatuocotyle (Monogenea, Dactylogyridae) parasitizing two species of Astyanax (Ostariophysi, Characidae) in southern Brazil. Acta Parasitologica, 59, 638–642. 10.2478/s11686-014-0283-6Search in Google Scholar PubMed

Gasques L.S., Graca R.J., Prioli S.M.A.P., Takemoto R.M., Prioli, A.J. 2016. Molecular characterization of Urocleidoides cuiabai and U. malabaricusi (Monogenea: Dactylogyridae) from the trahira fish Hoplias aff. malabaricus in the Parana River, Brazil. Journal of Helminthology, 90, 693–697. 10.1017/S0022149X15000966Search in Google Scholar PubMed

Huyse T., Malmberg G., Volckaert F.A.M. 2004. Four new species of Gyrodactylus von Nordmann, 1832 (Monogenea, Gyrodactylidae) on gobiid fishes: combined DNA and morphological analyses. Systematic Parasitology, 59, 103–120. 10.1023/B:SYPA.0000044427.81580.3Search in Google Scholar PubMed

Kearse M., Moir R., Wilson A., Stones-Havas S., Cheung M., Sturrock S., et al. 2012. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28, 1647–1649. 10.1093/bioinformatics/bts199Search in Google Scholar PubMed PubMed Central

Kritsky D.C., Fritts T.H. 1970. Monogenetic trematodes from Costa Rica with the proposal of Anacanthocotyle gen. n. (Gyrodactylidae: Isoancistrinae). Proceedings of the Helminthological Society of Washington, 37, 63–68Search in Google Scholar

Kritsky D.C., Leiby, P.D. 1972. Dactylogyridae (Monogenea) from the Freshwater Fish, Astyanax fasciatus (Cuvier), in Costa Rica, with descriptions of Jainus hexops sp. n., Urocleidoides costaricensis, and U. heteroancistrium combs, n. The Helminthological Society of Washington, 39, 227–230Search in Google Scholar

Kristsky D.C, Thatcher V.E., Boeger W.A. 1986. Neotropical Monogenea. 8. Revision of Urocleidoides (Dactylogyridae, Ancyrocephalinae). Proceedings of the Helminthological Society of Washington, 53, 1–37Search in Google Scholar

Littlewood D.T.J., Curini-Galletti M., Herniou E.A. 2000. The interrelationships of Proseriata (Platyhelminthes: Seriata) tested with molecules and morphology. Molecular Phylogenetics and Evolution, 16, 449–466. 10.1006/mpev.2000.0802Search in Google Scholar PubMed

Lockyer A.E., Olson P.D., Littlewood D.T.J. 2003. Utility of complete large and small subunit rRNA genes in resolving the phylogeny of the Neodermata (Platyhelminthes): implications and a review of the cercomer theory. Biological Journal of the Linnean Society, 78, 155–171. 10.1046/j.1095-8312.2003.00141.xSearch in Google Scholar

Mendoza-Franco E., Reina R.G., Torchin M.E. 2009. Dactylogyrids (Monogenoidea) parasitizing the gills of Astyanax spp. (Characidae) from panama and southeast Mexico, a new species of Diaphorocleidus and a proposal for Characithecium n. gen. Journal of Parasitology, 95, 46–55. 10.1645/GE-1592.1Search in Google Scholar PubMed

Mendoza-Franco E., Caspeta-Mandujano J.M., Salgado-Maldonado G. 2013. New species of Cacatuocotyle (Monogenoidea, Dactylogyridae) parasitizing the anus and the gill lamellae of Astyanax aeneus (Pisces, Ostariophysi: Characidae) from the Rio Lacantún basin in the Biosphere Reserve of Montes Azules, Chiapas, Mexico. Parasitology Research, 12, 199–205. 10.1007/s00436-012-3126-0Search in Google Scholar PubMed

Mendoza-Palmero C.A., Scholz T., Mendoza-Franco E.F., Kuchta R. 2012. New species and geographical records of dactylogyrids (Monogenea) of catfish (Siluriformes) from the Peruvian Amazonia. Journal of Parasitology, 98, 484–97. 10.1645/GE-2941.1Search in Google Scholar PubMed

Mendoza-Palmero C.A., Blasco-Costa I., Scholz T. 2015. Molecular phylogeny of Neotropical monogeneans (Platyhelminthes: Monogenea) from catfishes (Siluriformes). Parasite & Vectors, 8, 164. 10.1186/s13071-015-0767-8Search in Google Scholar PubMed PubMed Central

Mendoza-Palmero C.A., Blasco-Costa I., Henández-Mena, D., Pérez-Ponce de Léon, G. 2017. Parasciadicleithrum octofasciatum n. gen., n. sp. (Monogenoidea: Dactylogyridae), parasite of Rocio octofasciata (Regan) (Cichlidae: Perciformes) from Mexico characterised by morphological and molecular evidence. Parasitology International, 66, 152–162. 10.1016/j.parint.2017.01.006Search in Google Scholar PubMed

Monteiro C.M., Brasil-Sato M.C. 2014. A new species of Anacanthoroides and redescription of Apedunculata discoidea (Monogenoidea) parasitizing Prochilodus argenteus (Actinopterygii) from the São Francisco River, Brazil. Zootaxa, 3784, 259–266. 10.11646/zootaxa.3784.3.5Search in Google Scholar PubMed

Moreira J., Scholz T., Luque J. L. 2016. A new species of Diaphorocleidus (Monogenea: Ancyrocephalinae) from the gills of Argonectes robertsi (Characiformes) and new records of dactylogyrids parasitic on fishes from the Xingu River, Amazon Basin, Brazil. Zoologia, 33, 1–6. 10.1590/S1984-4689zool-20160022Search in Google Scholar

Müller M.I., Ceccarelli P.S., Ueta M.T. 2016. Supplementary studies on Anacanthorus penilabiatus and Mymarothecium viatorum (Monogenea: Dactylogyridae) from Piaractus mesopotamicus (Characiformes: Serrasalmidae) in Brazil. Acta Parasitologica, 61, 508–515. 10.1515/ap-2016-0067Search in Google Scholar PubMed

Narciso R.B., Brandão H., Yamada F.H., Benine, R.C., Silva R.J. 2014. A new species of Trinibaculum (Monogenea: Dactylogyridae) parasite of the gills of Astyanax fasciatus (cuvier, 1819) (Characiformes: Characidae) in a neotropical River, São Paulo State, Brazil. Neotropical Helminthology, 8, 85–95Search in Google Scholar

Nelson J.S. 2006. Fishes of the world. 4th edn. John Wiley & Sons, United States of America, pp. 601Search in Google Scholar

Řehulková E., Mendlová M., Šimková A. 2013. Two new species of Cichlidogyrus (Monogenea: Dactylogyridae) parasitizing the gills of African cichlid fishes (Perciformes) from Senegal: morphometric and molecular characterization. Parasitology Research, 112, 1399–1410. 10.1007/s00436-013-3291-9Search in Google Scholar PubMed

Reis R.E., Kullander S.O., Ferraris Jr. C.J. 2003. Check List of the Freshwater Fishes of South and Central America. EDIPUCRS, Porto Alegre, pp. 742Search in Google Scholar

Rossin M.A, Timi J. T. 2014. Characithecium (Monogenoidea: Dactylogyridae) parasitic on the Neotropical fish Oligosarcus jenynsii (Teleostei: Characidae) from the Pampasic region, Argentina, with the emendation of the genus. Zootaxa, 3893, 382–396. 10.11646/zootaxa.3893.3.4Search in Google Scholar PubMed

Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 12, 2725–2729. 10.1093/molbev/mst197Search in Google Scholar PubMed PubMed Central

Thatcher V.E. 2006. Amazon fish parasites. 2nd Edn. Pensoft, Bulgaria, pp. 508Search in Google Scholar

Received: 2017-7-4
Revised: 2017-12-13
Accepted: 2018-1-15
Published Online: 2018-4-13
Published in Print: 2018-6-26

© 2018 W. Stefański Institute of Parasitology, PAS